MOUSE HEPATITIS VIRUS-ASSOCIATED IMMUNE DYSFUNCTION

小鼠肝炎病毒相关的免疫功能障碍

• 批准号: 2282924
• 负责人: ABIGAIL L SMITH
• 金额: $ 17.55万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-30 至 1995-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2282924
• 关键词: B lymphocyte; CD4 molecule; CD8 molecule; T lymphocyte; animal viral hepatitis; antibody formation; antiviral antibody; athymic mouse; cellular immunity; communicable disease control; epizootiology; helper T lymphocyte; host organism interaction; immunopathology; immunotherapy; interferon gamma; interleukin 4; laboratory mouse; leukocyte activation /transformation; macrophage; murine hepatitis virus; natural killer cells; newborn animals; tissue /cell culture; virus antigen

Mouse hepatitis virus (MHV) infection of laboratory mice occurs at high prevalence and impacts heavily on many biomedical disciplines in which mice are commonly used. Infection of lymphocyte donors with MHV results in T cell dysfunction, as reflected by in vitro proliferation and cytokine production. However, the role of cytokines in the pathogenesis of MHV infection after exposure of mice by natural routes has not been addressed. Once the role of these molecules has been firmly established, efforts will be made to identify the cell type(s) secreting cytokines and the cell type(s) upon which they act. The long-term goal is the development of rational strategies for control and prevention of enzootic MHV in laboratory mouse colonies. The specific aims of the proposed research are: 1. to characterize further the role of cytokines in MHV pathogenesis, using cytokine immunotherapy or induction methods and cytokine deletion, and 2. to identify the cell types producing beneficial cytokines as well as those that are activated by these soluble mediators. The objective of the current proposal is to test the hypothesis that certain cytokines released by activated T cells, as well as other cell types, may mediate benign outcome of infection with MHV. Interferon gamma and interleukin 4 are the molecules of primary interest. Pilot data suggest that effector function of CD4+ T cells may be associated with pathology and negative outcome of MHV infection. These two aspects of T cell biology are difficult to separate; however, drug- and/or antibody-induced cell deletion combined with cytokine immunotherapy will begin to suggest whether our hypothesis has merit. The availability of genetically defined mice, recombinant cytokines, monoclonal antibodies reactive with these molecules and cell-specific drugs renders an approach to the in vivo interactions of virus and the immune system feasible.

小鼠肝炎病毒（MHV）感染在实验室小鼠中发生率高

项目成果

Susceptibility of laboratory mice to intranasal and contact infection with coronaviruses of other species.

实验小鼠对其他物种冠状病毒鼻内和接触感染的易感性。

• 发表时间: 1990
• 期刊: Laboratory animal science
• 作者: Barthold,SW;deSouza,MS;Smith,AL
• 通讯作者: Smith,AL

Characterization of accessory cell function during acute infection of BALB/cByJ mice with mouse hepatitis virus (MHV), strain JHM.

小鼠肝炎病毒 (MHV)、JHM 株急性感染 BALB/cByJ 小鼠期间辅助细胞功能的表征。

• 发表时间: 1991
• 期刊: Laboratory animal science
• 作者: deSouza,MS;Smith,AL
• 通讯作者: Smith,AL

The role of gamma interferon in infection of susceptible mice with murine coronavirus, MHV-JHM.

γ干扰素在易感小鼠感染鼠冠状病毒 MHV-JHM 中的作用。

• DOI: 10.1007/bf01316746
• 发表时间: 1991
• 期刊: Archives of virology
• 影响因子: 2.7
• 作者: Smith,AL;Barthold,SW;deSouza,MS;Bottomly,K
• 通讯作者: Bottomly,K

Role of host age and genotype in murine enterotropic coronavirus infection.

宿主年龄和基因型在鼠肠道冠状病毒感染中的作用。

• DOI: 10.1007/978-1-4615-2996-5_57
• 发表时间: 1993
• 期刊: Advances in experimental medicine and biology
• 作者: Barthold,SW;Smith,AL
• 通讯作者: Smith,AL

Responses of mice to murine coronavirus immunization.

小鼠对鼠冠状病毒免疫的反应。

• DOI: 10.1007/bf01309627
• 发表时间: 1992
• 期刊: Archives of virology
• 影响因子: 2.7
• 作者: Smith,AL;deSouza,MS;Finzi,D;Barthold,SW
• 通讯作者: Barthold,SW