MOUSE HEPATITIS VIRUS-ASSOCIATED IMMUNE DYSFUNCTION
MOUSE HEPATITIS VIRUS-ASSOCIATED IMMUNE DYSFUNCTION
批准号:
2282924
负责人:
ABIGAIL L SMITH
金额:
$17.55万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1988
资助国家:
美国
项目状态:
已结题
起止时间:
1988-09-30 至 1995-09-29
关键词:
B lymphocyte CD4 molecule CD8 molecule T lymphocyte animal viral hepatitis antibody formation antiviral antibody athymic mouse cellular immunity communicable disease control epizootiology helper T lymphocyte host organism interaction immunopathology immunotherapy interferon gamma interleukin 4 laboratory mouse leukocyte activation /transformation macrophage murine hepatitis virus natural killer cells newborn animals tissue /cell culture virus antigen
中文摘要
小鼠肝炎病毒(MHV)感染实验小鼠发生在高
在许多生物医学学科中,
通常使用。 用MHV感染淋巴细胞供体可导致T
细胞功能障碍,如体外增殖和细胞因子所反映的
生产 然而,细胞因子在MHV发病机制中的作用
小鼠通过自然途径暴露后的感染尚未得到解决。
一旦这些分子的作用被牢固地确立,
以鉴定分泌细胞因子的细胞类型,
他们所从事的工作。 长期目标是发展
控制和预防地方性MHV的合理策略
实验室小鼠群体。
本研究的具体目的是:1.为了进一步表征
细胞因子在MHV发病机制中的作用,使用细胞因子免疫疗法或
诱导方法和细胞因子缺失,和2.来识别细胞类型
产生有益的细胞因子以及那些被这些细胞因子激活的细胞因子,
可溶性介质
本提案的目的是检验以下假设:
活化的T细胞释放的某些细胞因子,以及其他细胞
型,可能介导MHV感染的良性结果。 干扰素γ
和白细胞介素4是主要感兴趣的分子。 导频数据
提示CD 4 + T细胞效应功能可能与
MHV感染的病理学和阴性结果。 T的这两个方面
细胞生物学难以分离;然而,药物和/或
抗体诱导的细胞缺失结合细胞因子免疫疗法将
开始暗示我们的假设是否有价值。 的可用性
基因定义小鼠,重组细胞因子,单克隆抗体
与这些分子和细胞特异性药物反应,
病毒和免疫系统的体内相互作用是可行的。
英文摘要
Mouse hepatitis virus (MHV) infection of laboratory mice occurs at high
prevalence and impacts heavily on many biomedical disciplines in which mice
are commonly used. Infection of lymphocyte donors with MHV results in T
cell dysfunction, as reflected by in vitro proliferation and cytokine
production. However, the role of cytokines in the pathogenesis of MHV
infection after exposure of mice by natural routes has not been addressed.
Once the role of these molecules has been firmly established, efforts will
be made to identify the cell type(s) secreting cytokines and the cell
type(s) upon which they act. The long-term goal is the development of
rational strategies for control and prevention of enzootic MHV in
laboratory mouse colonies.
The specific aims of the proposed research are: 1. to characterize further
the role of cytokines in MHV pathogenesis, using cytokine immunotherapy or
induction methods and cytokine deletion, and 2. to identify the cell types
producing beneficial cytokines as well as those that are activated by these
soluble mediators.
The objective of the current proposal is to test the hypothesis that
certain cytokines released by activated T cells, as well as other cell
types, may mediate benign outcome of infection with MHV. Interferon gamma
and interleukin 4 are the molecules of primary interest. Pilot data
suggest that effector function of CD4+ T cells may be associated with
pathology and negative outcome of MHV infection. These two aspects of T
cell biology are difficult to separate; however, drug- and/or
antibody-induced cell deletion combined with cytokine immunotherapy will
begin to suggest whether our hypothesis has merit. The availability of
genetically defined mice, recombinant cytokines, monoclonal antibodies
reactive with these molecules and cell-specific drugs renders an approach
to the in vivo interactions of virus and the immune system feasible.
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Susceptibility of laboratory mice to intranasal and contact infection with coronaviruses of other species.
实验小鼠对其他物种冠状病毒鼻内和接触感染的易感性。
DOI:
--
发表时间:
1990
期刊:
Laboratory animal science
影响因子:
--
作者:
[Barthold,SW, deSouza,MS, Smith,AL]
通讯作者:
Smith,AL
Characterization of accessory cell function during acute infection of BALB/cByJ mice with mouse hepatitis virus (MHV), strain JHM.
小鼠肝炎病毒 (MHV)、JHM 株急性感染 BALB/cByJ 小鼠期间辅助细胞功能的表征。
DOI:
--
发表时间:
1991
期刊:
Laboratory animal science
影响因子:
--
作者:
[deSouza,MS, Smith,AL]
通讯作者:
Smith,AL
The role of gamma interferon in infection of susceptible mice with murine coronavirus, MHV-JHM.
γ干扰素在易感小鼠感染鼠冠状病毒 MHV-JHM 中的作用。
DOI:
10.1007/bf01316746
发表时间:
1991
期刊:
Archives of virology
影响因子:
2.7
作者:
[Smith,AL, Barthold,SW, deSouza,MS, Bottomly,K]
通讯作者:
Bottomly,K
Role of host age and genotype in murine enterotropic coronavirus infection.
宿主年龄和基因型在鼠肠道冠状病毒感染中的作用。
DOI:
10.1007/978-1-4615-2996-5_57
发表时间:
1993
期刊:
Advances in experimental medicine and biology
影响因子:
--
作者:
[Barthold,SW, Smith,AL]
通讯作者:
Smith,AL
Responses of mice to murine coronavirus immunization.
小鼠对鼠冠状病毒免疫的反应。
DOI:
10.1007/bf01309627
发表时间:
1992
期刊:
Archives of virology
影响因子:
2.7
作者:
[Smith,AL, deSouza,MS, Finzi,D, Barthold,SW]
通讯作者:
Barthold,SW
共 8 条
CONTROLLING ALLERGENS AND COSTS IN THE ANIMAL FACILITY
-
批准号:6188580
-
项目类别:
-
资助金额:$32.06万
-
财政年份:1998
-
负责人:ABIGAIL L SMITH
-
依托单位:
HEALTH IMPROVEMENT & COST REDUCTION IN ANIMAL FACILITIES
-
批准号:6626153
-
项目类别:
-
资助金额:$41.18万
-
财政年份:1998
-
负责人:ABIGAIL L SMITH
-
依托单位:
HEALTH IMPROVEMENT & COST REDUCTION IN ANIMAL FACILITIES
-
批准号:6487045
-
项目类别:
-
资助金额:$40.72万
-
财政年份:1998
-
负责人:ABIGAIL L SMITH
-
依托单位:
NEWLY RECOGNIZED LYMPHOCYTOTROPIC PARVOVIRUS OF MICE
-
批准号:2285845
-
项目类别:
-
资助金额:$27.66万
-
财政年份:1995
-
负责人:ABIGAIL L SMITH
-
依托单位:
A NEWLY RECOGNIZED LYMPHOCYTOTROPIC PARVOVIRUS OF MICE
-
批准号:2669135
-
项目类别:
-
资助金额:$30.9万
-
财政年份:1995
-
负责人:ABIGAIL L SMITH
-
依托单位:
NEWLY RECOGNIZED LYMPHOCYTOTROPIC PARVOVIRUS OF MICE
-
批准号:2285846
-
项目类别:
-
资助金额:$28.57万
-
财政年份:1995
-
负责人:ABIGAIL L SMITH
-
依托单位:
A NEWLY RECOGNIZED LYMPHOCYTOTROPIC PARVOVIRUS OF MICE
-
批准号:2379975
-
项目类别:
-
资助金额:$29.71万
-
财政年份:1995
-
负责人:ABIGAIL L SMITH
-
依托单位:
RENOVATION OF A DIAGNOSTIC VIROLOGY LABORATORY
-
批准号:3059322
-
项目类别:
-
资助金额:$40.15万
-
财政年份:1992
-
负责人:ABIGAIL L SMITH
-
依托单位:
MOUSE HEPATITIS VIRUS-ASSOCIATED IMMUNE DYSFUNCTION
-
批准号:3451117
-
项目类别:
-
资助金额:$17.49万
-
财政年份:1988
-
负责人:ABIGAIL L SMITH
-
依托单位:
MOUSE HEPATITIS VIRUS-ASSOCIATED IMMUNE DYSFUNCTION
-
批准号:3451120
-
项目类别:
-
资助金额:$14.18万
-
财政年份:1988
-
负责人:ABIGAIL L SMITH
-
依托单位:
MOUSE HEPATITIS VIRUS-ASSOCIATED IMMUNE DYSFUNCTION
-
批准号:3451118
-
项目类别:
-
资助金额:$16.73万
-
财政年份:1988
-
负责人:ABIGAIL L SMITH
-
依托单位:
MOUSE HEPATITIS VIRUS-ASSOCIATED IMMUNE DYSFUNCTION
-
批准号:3421494
-
项目类别:
-
资助金额:$16.88万
-
财政年份:1988
-
负责人:ABIGAIL L SMITH
-
依托单位:
MOUSE HEPATITIS VIRUS-ASSOCIATED IMMUNE DYSFUNCTION
-
批准号:3451119
-
项目类别:
-
资助金额:$14.68万
-
财政年份:1988
-
负责人:ABIGAIL L SMITH
-
依托单位:
海外基金