Development of 13C-retinol isotope dilution techniques

13C-视黄醇同位素稀释技术的开发

• 批准号: 6661087
• 负责人: SHERRY A TANUMIHARDJO
• 金额: $ 2.63万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6661087
• 关键词: Macaca mulatta; carbon; clinical research; diagnosis design /evaluation; diagnostic tests; dietary supplements; dosage; human subject; isotope dilution method; liver; mature animal; method development; nutrient requirement; nutrition; nutrition disorder diagnosis; nutrition related tag; patient oriented research; pharmacology; retinoids; stable isotope; swine; toxicology; vitamin A deficiency; weanling animal; young adult human (21-34)

DESCRIPTION (provided by applicant): Improved, simplified methods to determine vitamin A status are needed to accurately assess both individuals and populations at risk of either vitamin A deficiency or toxicity. The vitamin A statuses of women and children in lower socioeconomic groups in both the underdeveloped world and the United States are compromised. Multiple reasons for this compromised status include low dietary intakes, increased demands during pregnancy and lactation and recurrent infections. The 13C-retinol isotope dilution (13C-RID) test, first developed in the rodent model using 13C4-retinyl acetate and gas chromatography-combustion-isotope ratio mass spectrometry (GCCIRMS), is sensitive and accurate. The major goals of this project are to validate the 13C-RID test for use in human population groups to assess total body reserves of vitamin A and determine vitamin A requirements of young adults. The success of this project will provide a sensitive tool for investigators to evaluate the vitamin A status of individuals and population groups using minimal sample sizes. The test has direct application to determining the outcome of intervention strategies aimed at improving vitamin A status and in determining vitamin A needs of humans and animals throughout their life cycles. The proposed studies will for the first time: (1) validate the 13C-RID test to assess a sub-toxic vitamin A status in non-human primates; (2) validate the 13C-RID test to assess sub-clinical vitamin A deficiency in the weanling pig; (3) refine the 13C-RID test for use in humans by looking at different dose levels and blood sampling times; and (4) investigate the use of the 13C-RID test in a human intervention study designed to look at vitamin A requirements in young adults.

描述(由申请人提供)：需要改进、简化的确定维生素A状态的方法，以准确评估处于维生素A缺乏或中毒风险的个人和人群。在不发达世界和美国，处于较低社会经济群体中的妇女和儿童的维生素A状况都受到了影响。造成这种状况的多种原因包括饮食摄入量低、孕期和哺乳期需求量增加以及反复感染。13C-视黄醇同位素稀释法(13C-RID)首次在啮齿动物模型中使用13C4-维甲酸乙酯和气相色谱-燃烧-同位素比值质谱仪(GCCIRMS)建立，具有灵敏和准确的特点。该项目的主要目标是验证13C-RID测试在人类群体中的使用，以评估人体内维生素A的总储量，并确定年轻人的维生素A需求。该项目的成功将为研究人员提供一个敏感的工具，利用最小的样本量来评估个人和人群的维生素A状况。该测试直接应用于确定旨在改善维生素A状况的干预策略的结果，以及确定人类和动物在其整个生命周期中的维生素A需求。拟议的研究将首次：(1)验证13C-RID测试以评估非人类灵长类动物的亚毒性维生素A状态；(2)验证13C-RID测试以评估断奶猪的亚临床维生素A缺乏；(3)通过观察不同的剂量水平和采血时间来改进13C-RID测试以用于人类；以及(4)研究13C-RID测试在一项旨在研究年轻人维生素A需求的人类干预研究中的使用情况。