Polymer Membrane Ion/Polyion Sensors: New Frontiers

聚合物膜离子/聚离子传感器：新领域

基本信息

批准号：
6468313
负责人：
MARK E MEYERHOFF
金额：
$ 21.55万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
1981
资助国家：
美国
起止时间：
1981-04-01 至 2006-03-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The continued development, study, and bioanalytical applications of novel polyion and anion selective polymer membrane/film-based electrochemical and optical sensors are proposed. Research during the next phase of this long-term project will build upon several significant discoveries/advances made during the most recent project period relating to potentiometric polyion sensing and new chemistries for anion-selective sensors. These include dramatically lowering the detection limits toward biomedically important polyions (e.g., heparin, protamine, etc.) via use of a novel rotating potentiometric membrane electrode configuration, and 2) uncovering a unique hydroxide ion bridged dimer/monomer equilibrium that can exist for certain metalloporphyrins when used as ionophores in organic polymer films for devising anion sensing electrodes. Future efforts will include both fundamental and applied studies in both the polyion and anion sensing areas. Specific goals for the polyion sensor efforts include: a) better understanding the nature (size, stoichiometry, etc.) of the ion-exchanger/polyion complexes that form within the polymer membranes used to fabricate such devices; b) demonstrating broader bioanalytical applications of the new, more sensitive rotating polyion sensing membrane electrode configuration; c) investigating the use of polyion sensors (including rotating arrangement) to detect polycationic dendrimers and their interaction with DNA; and d) further development of a completely new and rapid homogeneous immunoassay method for detecting of small, clinically and environmentally important analytes using polyions as labels. In the area of anion sensors, efforts will focus on establishing which metalloporphyrins can spontaneously form hydroxide ion bridged dimer structures within polymeric films, and determining how these reactions affect the response properties of anion selective electrodes formulated with such ionophores. In addition, studies will be undertaken to utilize this novel chemistry to devise completely new polymer film-based optical sensors for anions and neutral species (amines, gases, etc.) based on the ability of such ligating species to break metalloporphyrins dimers into monomers within the organic films, yielding a large shift in the lambdamax of the Soret band. Investigations of electrochemical anion sensors based on a new type of lipophilic dendntic anion-exchanger structure are also proposed. It is anticipated that this research will continue to provide the analytical community with a wide array of new and/or improved chemical sensors as well as novel sensor-based methods that will have immediate applications as tools for basic research and within modem clinical and environmental test instrumentation.

描述（由申请人提供）：持续的发展，研究和新型Polyion和阴离子选择性聚合物的生物分析应用提出了基于膜/膜的电化学和光学传感器。研究在这个长期项目的下一阶段，将建立在几个在最近的项目期间取得的重大发现/进步与电位计量计的感应和新化学有关阴离子选择传感器。这些包括大大降低检测限制在生物医学上重要的多元（例如肝素，精蛋白等）的限制通过使用新型的旋转电位计量膜电极构型， 2）发现独特的氢氧化离子桥接二聚体/单体平衡当用作有机的离子载体时，可能存在某些金属酚蛋白用于设计阴离子传感电极的聚合物膜。未来的努力会在Polyion和阴离子中都包括基本研究和应用研究感应区域。 Polyion传感器工作的具体目标包括：a）更好了解该的性质（大小，化学计量等）在聚合物膜内形成的离子 - 交换器/Polyion络合物曾经形成制造此类设备； b）展示更广泛的生物分析应用新的，更敏感的旋转Polyion感测膜电极配置; c）研究使用Polyion传感器（包括旋转排列）以检测多阳离子树状聚合物及其与DNA的相互作用； d）进一步开发一个全新的快速同质用于检测小型，临床和环境的免疫测定法使用Polyions作为标签的重要分析物。在阴离子传感器的区域，努力将集中于确定哪种金属磷脂可以自发形成聚合物膜中的氢氧化离子桥接二聚体结构，并形成确定这些反应如何影响阴离子的响应特性选择性电极用这种离子载体配制。此外，研究将要利用这种新颖的化学反应来设计全新的聚合物基于膜的阴离子和中性物种（胺，气体等）的光传感器基于此类连接物种破坏金属核苷二聚体的能力进入有机膜中的单体，在Lambdamax中产生了很大的变化 soret乐队。基于A的电化学阴离子传感器的调查还提出了新型的亲脂性阴离子 - 交换器结构。它预计这项研究将继续提供分析具有广泛的新和/或改进的化学传感器以及基于新型传感器的方法将立即应用作为工具基础研究以及调制解调器临床和环境测试仪器。