Polymer Membrane Ion/Polyion Sensors: New Frontiers

描述(申请人提供)：继续发展、学习和 新型聚阴离子选择性聚合物在生物分析中的应用 提出了基于膜/膜的电化学和光学传感器。研究 在这个长期项目的下一阶段，将在以下几个基础上 在最近项目期内取得的重大发现/进展 与电位型多离子传感有关的新化学 阴离子选择性传感器。这些措施包括大幅降低检测 对生物医学上重要的多离子(如肝素、鱼精蛋白等)的限制 通过使用一种新型的旋转电位计膜电极配置， 以及2)揭示了独特的氢氧离子桥联二聚体/单体平衡 当用作有机中的离子载体时，某些金属卟啉可以存在 用于设计阴离子传感电极的聚合物薄膜。未来的努力将 包括聚离子和阴离子的基础和应用研究 感应区。多离子传感器工作的具体目标包括：a)更好 了解自然(大小、化学计量等)的 在聚合物膜内形成的离子交换剂/多离子络合物，用于 制造这种装置；b)展示更广泛的生物分析应用 新型、更灵敏的旋转式聚离子传感膜电极 配置；c)调查多离子传感器的使用(包括旋转 排列)检测多阳离子树枝状大分子及其与DNA的相互作用； 和d)进一步开发一种全新的、快速的同质化 检测微小、临床和环境污染的免疫分析方法 使用多离子作为标记的重要分析物。在阴离子传感器领域， 努力将重点放在确定哪些金属卟啉可以自发地 在聚合物膜内形成氢氧离子桥联二聚体结构，以及 确定这些反应如何影响阴离子的响应性质 用这种离子载体制成的选择性电极。此外，研究还将 致力于利用这种新的化学来设计全新的聚合物 用于阴离子和中性物质(胺、气体等)的薄膜光学传感器 基于这种连接物种破坏金属卟啉二聚体的能力 转化为有机薄膜中的单体，在LambDamax中产生了很大的位移 索雷特乐队的成员。一种新型的电化学阴离子传感器的研究 还提出了一种新型的亲脂性树枝状阴离子交换结构。它 预计这项研究将继续提供分析 社区拥有一系列新的和/或改进的化学传感器以及 基于传感器的新方法，将立即作为工具应用于 基础研究和现代临床和环境试验 仪器仪表。

DESCRIPTION (provided by applicant): The continued development, study, and bioanalytical applications of novel polyion and anion selective polymer membrane/film-based electrochemical and optical sensors are proposed. Research during the next phase of this long-term project will build upon several significant discoveries/advances made during the most recent project period relating to potentiometric polyion sensing and new chemistries for anion-selective sensors. These include dramatically lowering the detection limits toward biomedically important polyions (e.g., heparin, protamine, etc.) via use of a novel rotating potentiometric membrane electrode configuration, and 2) uncovering a unique hydroxide ion bridged dimer/monomer equilibrium that can exist for certain metalloporphyrins when used as ionophores in organic polymer films for devising anion sensing electrodes. Future efforts will include both fundamental and applied studies in both the polyion and anion sensing areas. Specific goals for the polyion sensor efforts include: a) better understanding the nature (size, stoichiometry, etc.) of the ion-exchanger/polyion complexes that form within the polymer membranes used to fabricate such devices; b) demonstrating broader bioanalytical applications of the new, more sensitive rotating polyion sensing membrane electrode configuration; c) investigating the use of polyion sensors (including rotating arrangement) to detect polycationic dendrimers and their interaction with DNA; and d) further development of a completely new and rapid homogeneous immunoassay method for detecting of small, clinically and environmentally important analytes using polyions as labels. In the area of anion sensors, efforts will focus on establishing which metalloporphyrins can spontaneously form hydroxide ion bridged dimer structures within polymeric films, and determining how these reactions affect the response properties of anion selective electrodes formulated with such ionophores. In addition, studies will be undertaken to utilize this novel chemistry to devise completely new polymer film-based optical sensors for anions and neutral species (amines, gases, etc.) based on the ability of such ligating species to break metalloporphyrins dimers into monomers within the organic films, yielding a large shift in the lambdamax of the Soret band. Investigations of electrochemical anion sensors based on a new type of lipophilic dendntic anion-exchanger structure are also proposed. It is anticipated that this research will continue to provide the analytical community with a wide array of new and/or improved chemical sensors as well as novel sensor-based methods that will have immediate applications as tools for basic research and within modem clinical and environmental test instrumentation.