Lectin based open tubular micro-reactors for probing protein-protein binding

基于凝集素的开放管式微反应器用于探测蛋白质-蛋白质结合

• 批准号: DP140104323
• 负责人: Prof Brett Paull
• 金额: $ 22.23万
• 依托单位: University of Tasmania
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-01-01 至 2017-06-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP140104323
• 关键词: Lectin; based; open; tubular; micro

This project seeks to develop non-invasive technology for the measurement and quantitation of lectin-carbohydrate binding events, in the first instance glycopeptides and glycoproteins. The goal is not only to provide accurate protein-protein association and dissociation constant data within the developed system, but to do so within an enclosed micro-fluidic environment, with the added advantages of also providing ‘trap and release’ extraction capabilities, and being easily coupled to both chromatographic and mass spectrometry systems.

该项目旨在开发非侵入性技术来测量和定量凝集素-碳水化合物结合事件，首先是糖肽和糖蛋白。其目标不仅是在开发的系统中提供准确的蛋白质-蛋白质缔合和解离常数数据，而且还在封闭的微流体环境中做到这一点，另外还有一个优势，即还提供“捕获和释放”提取能力，并且易于与色层和质谱分析系统耦合。