Instrumental Analysis

仪器分析

• 批准号: 6542172
• 负责人: George H Weiss
• 金额: --
• 依托单位: CENTER FOR INFORMATION TECHNOLOGY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6542172
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; clinical biomedical equipment; diagnosis design /evaluation; digital imaging; image processing; optics; spectrometry

The purpose of this project is to develop theory for the interpretation of biomedical and chemical measurement techniques. At present we have worked on two such techniques: single-molecule spectroscopy (SMS) and optical spectroscopy applied to estimate optical tissue parameters to distinguish between normal and abnormal tissues. In the past year we have continued our development of the theory of SMS applied to two-state randomly interconverting systems. The theory of such systems has been developed, in which one rate constant fluctuates randomly in response to environmental fluctuations. We have also developed statistical theory for the error in estimates of rate constants in two-state systems. These estimates permit the experimenter to optimize such experiments insofar as is possible. The general theory has also been appliied to study techniques for parameter estimation in two-state systems in which resolution is so poor that the data is virtually uncorrelated. Using data provided by the laboratory of Dr. Sergey Bezrukov (NICHD) we have shown that an adaptation of our theory of two-state systems is able to deal very well with poor quality data. A second project is to develop a theoretical framework for optical imaging methods for biomedical applications. We are currently developing theory to characterize the trajectory of laser-injected photons in continuous wave reflectance measurements.

该项目的目的是发展生物医学和化学测量技术的解释理论。目前，我们已经在两个这样的技术：单分子光谱（SMS）和光学光谱应用于估计光学组织参数，以区分正常和异常组织。 在过去的一年里，我们继续发展了应用于两态随机互变系统的SMS理论。这种系统的理论已经发展起来，其中一个速率常数随环境波动而随机波动。我们还发展了统计理论的错误估计的速率常数在两个状态系统。这些估计允许实验者尽可能地优化这些实验。一般理论也被应用于研究两状态系统的参数估计技术，其中分辨率很差，数据实际上是不相关的。使用谢尔盖·别兹鲁科夫博士（NICHD）实验室提供的数据，我们已经证明，我们的双态系统理论的适应能够很好地处理质量差的数据。 第二个项目是为生物医学应用的光学成像方法建立一个理论框架。我们目前正在发展理论，以表征连续波反射率测量中激光注入光子的轨迹。