The Amygdala and Amphetamine Self-Administration

杏仁核和安非他明自我管理

• 批准号: 6668460
• 负责人: Mary Eileen Cain
• 金额: $ 4.48万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-10-01 至 2004-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6668460
• 关键词: amphetamines; amygdala; arousal; behavioral /social science research tag; gene expression; ibotenate; immunocytochemistry; laboratory rat; neuropharmacology; neurophysiology; neurotoxicology; postdoctoral investigator; protooncogene; psychopharmacology; self medication

DESCRIPTION (provided by applicant): Drug self-administration in rodents is a useful technique to examine the mechanisms and potential treatment of stimulant abuse. Previous research has shown that stimulant self-administration is greater in rats that are highly active in a novel environment (high responders) compared to rats that are relatively inactive (low responders). In addition, stimulant self-administration is greater in rats raised in an isolated environment compared to rats raised in an enriched environment. The experiments proposed in the present application will determine if rats that are prone to self-administer amphetamine, due to genetic or environmental factors, share a common neural profile of an overactive arousal system. The overall mechanistic framework for the proposed set of experiments is based on the role of the central nucleus of the amygdala (ACe) in regulating arousal due to its extensive cortical and subcortical projections. We hypothesize that the ACe is more active in high responder rats compared to low responder rats and in rats reared in an isolated condition compared to an enriched condition. The effects of ibotenic acid lesions of the ACe on subsequent amphetamine self-administration will be measured. In addition, the amount of c-Fos labeling will be measured in the ACe to determine if the ACe has greater activity in those rats prone to self-administer amphetamine. The findings from these experiments are intended to advance the understanding of vulnerability for stimulant abuse, and will potentially lead to better prevention and treatment interventions.

描述（由申请人提供）： 啮齿动物中的药物自我给药是一种有用的技术，可用于检查刺激性滥用的机制和潜在治疗方法。先前的研究表明，与相对不活跃的大鼠相比，在新型环境（高响应者）中高度活跃的大鼠中，刺激性的自我给药更大。此外，与在富集环境中饲养的大鼠相比，在孤立环境中饲养的大鼠中的刺激性自我给药更大。本应用程序中提出的实验将确定由于遗传或环境因素而容易自动辅助苯丙胺的大鼠，它具有过度活跃的唤醒系统的共同神经特征。拟议的一组实验的总体机械框架是基于杏仁核（ACE）中央核在调节其广泛的皮质和皮层下的投影而在调节唤醒中的作用。我们假设，与低响应者大鼠相比，与富含条件相比，与低响应者大鼠相比，ACE在高响应者大鼠中更为活跃。将测量ACE的依Botenic酸病变对随后的苯丙胺自我给药的影响。另外，将在ACE中测量C-FOS标记的量，以确定ACE在容易自动辅助苯丙胺的大鼠中是否具有更大的活性。这些实验的发现旨在提高人们对刺激性滥用的脆弱性的理解，并有可能导致更好的预防和治疗干预措施。