Structure-function relations in Rieske-type proteins

Rieske型蛋白质的结构-功能关系

• 批准号: 6699926
• 负责人: SERGEI A DIKANOV
• 金额: $ 22.52万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6699926
• 关键词: X ray crystallography; bacterial proteins; chemical structure; electron spin resonance spectroscopy; ferredoxin; iron sulfur protein; membrane proteins; oxidation; photosynthetic bacteria; physical model; protein structure function; site directed mutagenesis

Iron-sulfur proteins are ubiquitous in nature and occur in most metabolic pathways, and an understanding of the parameters determining their physico-chemical properties is a central problem of biochemistry. Among the most interesting are the 2Fe-2S clusters that have as a common feature the liganding of the irons by two cysteines and two histidines. These include the Rieske iron-sulfur protein (ISP) of the bc1 (and related) complexes, and several Rieske-type bacterial proteins, including the archaeal sulredoxin (SDX) of Sulfolobus sp. Strain 7 and Rieske-type ferredoxin (ARF) from S. solfaricus to be investigated in this project. A remarkable feature of these proteins is the wide variation in redox potentials, from -100 to 300 mV, exhibited by the 2Fe-2S clusters, despite their similar ligation. Recent crystallographic structures show a similar orientation of ligands in the cluster binding domain in proteins at both extremes of this range. This implies that the redox potentials and protolytic properties of each particular cluster are controlled by other unique features of the protein environment. To understand how the proteins function, the factors influencing these parameters must be determined at the atomic level for each protein. We propose to investigate the protein environment of the three Rieske-type clusters above by using advanced magnetic resonance techniques, with an emphasis on 2-D ESEEM to explore the interaction between the paramagnetic centers and nuclear spins in the neighborhood. We will analyze the data so as to provide structural information, and compared this with the crystallographic structured of ISP, and of naphthalene- 1,2-dioxygenase. The spectroscopic results will provide constraints to allow precise determination of the cluster environment including coordination of histidine and cysteine ligands, presence of hydrogen bonds and non-coordinated nitrogens, and accessibility of solvent. By performing similar experiments with mutant strains generated by molecular engineering, we anticipate that we will be able to identify the local features of the protein environment that control the redox and protolytic properties of the clusters, their role in reaction mechanisms, and the changes that produce functional modification in different strains. The results will answer some fundamental questions about structural factors controlling the redox potentials of Rieske-type proteins, and provide insights to similar questions in other redox proteins.

铁硫蛋白在自然界中普遍存在，并出现在大多数代谢途径中，了解决定其理化性质的参数是生物化学的中心问题。 其中最有趣的是2Fe-2S簇，其共同特征是由两个半胱氨酸和两个组氨酸配位铁。 这些蛋白质包括bc 1（及相关）复合物的Rieske铁硫蛋白（ISP），以及几种Rieske型细菌蛋白质，包括硫化叶菌属菌株7的古细菌硫氧还蛋白（SDX）和来自S.在这个项目中要调查的Solfaricus。 这些蛋白质的一个显著特征是氧化还原电位的广泛变化，从-100至300 mV，由2Fe-2S簇表现出，尽管它们类似的连接。 最近的晶体学结构显示出在该范围的两个极端的蛋白质中的簇结合结构域中的配体的类似取向。 这意味着每个特定簇的氧化还原电位和质子水解性质由蛋白质环境的其他独特特征控制。 为了了解蛋白质的功能，必须在原子水平上确定影响这些参数的因素。 我们建议通过使用先进的磁共振技术来研究上述三种Rieske型簇的蛋白质环境，重点是2-D ESEEM来探索顺磁中心与邻近核自旋之间的相互作用。 我们将分析数据，以便提供结构信息，并将其与ISP和萘-1，2-双加氧酶的晶体结构进行比较。 光谱结果将提供限制，以允许精确确定的集群环境，包括协调的组氨酸和半胱氨酸配体，存在的氢键和非协调的氮，和溶剂的可及性。 通过对分子工程产生的突变株进行类似的实验，我们预计我们将能够确定蛋白质环境的局部特征，这些特征控制簇的氧化还原和质子水解性质，它们在反应机制中的作用，以及在不同菌株中产生功能修饰的变化。 这些结果将回答一些关于结构因素控制Rieske型蛋白质氧化还原电位的基本问题，并为其他氧化还原蛋白质中的类似问题提供见解。