DNA BASE EXCISION REPAIR IN PLASMODIUM FALCIPARUM

恶性疟原虫 DNA 碱基切除修复

基本信息

  • 批准号:
    6704235
  • 负责人:
  • 金额:
    $ 35.78万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2001
  • 资助国家:
    美国
  • 起止时间:
    2001-05-01 至 2006-03-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION: (provided by the applicant): DNA replication and repair, processes that are essential for cellular proliferation and the maintenance of genome integrity, have gone largely unexplored in parasitology. The replication and control of DNA repair in Plasmodium falciparum are likely to have a number of differences from the mammalian cell. The rapid replication as trophozoites mature into schizonts and the three rounds of replication that microgametes accomplish in 10 minutes at exflagellation are both unusual. For this reason, a basic understanding of these processes in the parasite is an important goal. Our groundbreaking investigations revealed that Plasmodium falciparum (Pj) has DNA repair capabilities, and that the removal of uracil and the repair of abasic sites in DNA are exclusively by a long-patch base excision repair (BER) pathway, which is remarkably different from mammalian cells. Important quantitative differences between the enzymology of the BER pathway in P. falciparum and mammalian cells have been established. The short-term goal of this proposal is to define the fundamental reaction of BER in Plasmodium falciparum. The essential proteins in the parasite BER pathway have been identified and the over expression of the enzymes in bacteria will facilitate their biochemical characterization. These enzymes include Pf AP endonuclease, Pf flap endonuclease (Pf FEN-1) and Pf DNA ligase. Expression of these enzymes is part of our plan to reconstitute the entire parasite BER pathway for studying the individual contributions of each of the key enzymes to the long-patch repair process. A reconstituted BER system will afford future study of the various protein-protein interactions, mechanisms, associations and biochemical reactions under controlled situations of BER. The ultimate goal of these studies is to identify the Plasmodium excision repair genes, determine their biological and biochemical function and assess their role in maintaining the parasite genome. Completion of the proposed aims in this project will provide important, new information about a neglected area of parasite biology, and possibly the opportunity to exploit differences in DNA repair between P. falciparum and mammalian cells to develop new strategies for antimalarial therapy.
描述:(由申请人提供):DNA复制和修复,过程

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Theodore F Taraschi其他文献

Theodore F Taraschi的其他文献

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{{ truncateString('Theodore F Taraschi', 18)}}的其他基金

Vivarium Modernization with Digital Ventilated Cages to Enhance Research Capacity and Reproducibility, and Provide Cage Environment Monitoring for Improved Operational Efficiency and Animal Welfare
采用数字通风笼进行现代化改造,以提高研究能力和再现性,并提供笼环境监测,以提高运营效率和动物福利
  • 批准号:
    10533591
  • 财政年份:
    2022
  • 资助金额:
    $ 35.78万
  • 项目类别:
Evaluation of Small Molecule Inhibitors of Hemoglobin Transport as Antimalarials
血红蛋白转运小分子抑制剂作为抗疟药的评价
  • 批准号:
    8114424
  • 财政年份:
    2011
  • 资助金额:
    $ 35.78万
  • 项目类别:
Evaluation of Small Molecule Inhibitors of Hemoglobin Transport as Antimalarials
血红蛋白转运小分子抑制剂作为抗疟药的评价
  • 批准号:
    8320098
  • 财政年份:
    2011
  • 资助金额:
    $ 35.78万
  • 项目类别:
CYTOSTOME-FOOD VACUOLE INTERACTIONS IN PLASMODIUM FALCIPARUM
恶性疟原虫中细胞造口术-食物泡的相互作用
  • 批准号:
    8172288
  • 财政年份:
    2010
  • 资助金额:
    $ 35.78万
  • 项目类别:
DNA Mismatch Repair and Malaria Drug Resistance
DNA 错配修复和疟疾耐药性
  • 批准号:
    7472182
  • 财政年份:
    2008
  • 资助金额:
    $ 35.78万
  • 项目类别:
DNA Mismatch Repair and Malaria Drug Resistance
DNA 错配修复和疟疾耐药性
  • 批准号:
    7649239
  • 财政年份:
    2008
  • 资助金额:
    $ 35.78万
  • 项目类别:
CORE--MAGNETIC RESONANCE AND ELECTRON SPIN RESONANCE FACILITY
核心——磁共振和电子自旋共振装置
  • 批准号:
    6563163
  • 财政年份:
    2001
  • 资助金额:
    $ 35.78万
  • 项目类别:
DNA BASE EXCISION REPAIR IN PLASMODIUM FALCIPARUM
恶性疟原虫 DNA 碱基切除修复
  • 批准号:
    6632428
  • 财政年份:
    2001
  • 资助金额:
    $ 35.78万
  • 项目类别:
ALCOHOL ACTION ON MEMBRANE STRUCTURES
酒精对膜结构的作用
  • 批准号:
    6563154
  • 财政年份:
    2001
  • 资助金额:
    $ 35.78万
  • 项目类别:
DNA BASE EXCISION REPAIR IN PLASMODIUM FALCIPARUM
恶性疟原虫 DNA 碱基切除修复
  • 批准号:
    6371036
  • 财政年份:
    2001
  • 资助金额:
    $ 35.78万
  • 项目类别:

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