COBRE in Stem Cell Biology and Regenerative Medicine

COBRE 在干细胞生物学和再生医学中的应用

• 批准号: 6700074
• 负责人: JOSEPH M VERDI
• 金额: $ 219.62万
• 依托单位: MAINEHEALTH
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6700074
• 关键词: cell biology; cell differentiation; cell growth regulation; cell population study; cooperative study; developmental genetics; health science research support; regeneration; stem cells

DESCRIPTION (provided by applicant): Stem cells are defined as multipotent, self-renewing undifferentiated cells capable of differentiating into numerous cell lineages. A great deal of interest has focused on the potential therapeutic application of such stem cell populations, not only to repair or replace damaged tissues, but as model systems to understand basic developmental processes leading to normal and abnormal cellular development. As a result, this application requests support from the IDeA program to establish a COBRE in Stem Cell Biology and Regenerative Medicine and involves the collaborative interests of a group of four (non-RO1 funded) junior investigators, including 1 from The Jackson Laboratory, under the mentorship of an established investigator in the field of Stem Cell Biology within the Center for Regenerative Medicine at the Maine Medical Center. The intent of including The Jackson Laboratory is to establish and bridge long-term collaborative research programs in the state of Maine. The theme of the application involves investigations into the signals, receptors, signaling cascades, and transcriptional regulatory circuits that control stem cell development. The motives for this application include the use of this award to (i) support, develop, and advance promising young investigators in the field of Stem Cell Biology, (ii) expand existing institutional resources to create a critical mass of mechanism-oriented junior and senior investigators in this area, (iii) establish and expand core resources in Stem Cell Isolation, Pathology, and Molecular Genetics to support ongoing and future investigator needs, and (iv) provide resources requisite for the establishment of an internationally recognized non-profit research institution in the broad area of Stem Cell Biology, with the potential to ultimately apply this information to the field of Regenerative Medicine. The application is comprised of four projects that were chosen in the field of Stem Cell Biology and are the crux of current investigations in the field. These projects include (i) The role of pMesogenin in the mesodermal differentiation of embryonic stem cells, (ii) Notch signaling events in the self-renewal of embryonic stem cells, (iii) The role of Wnt signaling in the preimplantation embryo, and (iv) VEGFR-Notch-Prox resolution of lymph vessel fate. Program expansion beginning in 2003 will include the recruitment of at least 5 investigators with genetic, cellular, and molecular expertise to address mechanistic questions in the general areas of (i) stem cell plasticity, (ii) Hedgehog signaling events in lineage specification, (iii) computational biology, (iv) non-murine models of development, (v) transplantation biology, and (vi) genomics, proteomics, and systems biology. It is anticipated that this award will not only enable the Center for Regenerative Medicine to achieve its goal at a more rapid and efficient rate, but the insight derived from these investigations may significantly impact the field of Stem Cell Biology and Regenerative Medicine.

描述（由申请人提供）： 干细胞被定义为多能、自我更新的未分化细胞，能够分化成多种细胞谱系。 人们对此类干细胞群的潜在治疗应用产生了极大的兴趣，不仅用于修复或替换受损组织，而且作为模型系统来了解导致正常和异常细胞发育的基本发育过程。 因此，本申请请求 IDeA 计划的支持，以建立干细胞生物学和再生医学领域的 COBRE，并涉及由四名（非 RO1 资助）初级研究人员组成的小组的合作利益，其中包括来自杰克逊实验室的一名研究人员，在缅因州医学中心再生医学中心干细胞生物学领域的一位资深研究人员的指导下。 纳入杰克逊实验室的目的是在缅因州建立和桥接长期合作研究项目。 该应用程序的主题涉及对控制干细胞发育的信号、受体、信号级联和转录调节电路的研究。 该申请的动机包括利用该奖项来（i）支持、培养和推进干细胞生物学领域有前途的年轻研究人员，（ii）扩大现有机构资源，以在该领域培养一批以机制为导向的初级和高级研究人员，（iii）建立和扩大干细胞分离、病理学和分子遗传学领域的核心资源，以支持当前和未来研究人员的需求，以及（iv）为建立干细胞生物学中心提供必要的资源。 国际公认的干细胞生物学广泛领域的非营利研究机构，有可能最终将这些信息应用于再生医学领域。 该应用程序由干细胞生物学领域选择的四个项目组成，是该领域当前研究的关键。 这些项目包括 (i) pMesogenin 在胚胎干细胞中胚层分化中的作用，(ii) Notch 信号传导事件在胚胎干细胞自我更新中的作用，(iii) Wnt 信号传导在植入前胚胎中的作用，以及 (iv) 淋巴管命运的 VEGFR-Notch-Prox 解析。 2003 年开始的计划扩展将包括招募至少 5 名具有遗传、细胞和分子专业知识的研究人员，以解决以下一般领域的机械问题：(i) 干细胞可塑性，(ii) 谱系规范中的 Hedgehog 信号事件，(iii) 计算生物学，(iv) 非鼠发育模型，(v) 移植生物学，以及 (vi) 基因组学、蛋白质组学和系统生物学。 预计该奖项不仅将使再生医学中心能够更快、更高效地实现其目标，而且从这些研究中获得的见解可能会对干细胞生物学和再生医学领域产生重大影响。