COBRE in Stem Cell Biology and Regenerative Medicine

COBRE 在干细胞生物学和再生医学中的应用

• 批准号: 6805267
• 负责人: JOSEPH M VERDI
• 金额: $ 219.51万
• 依托单位: MAINEHEALTH
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6805267
• 关键词: biological signal transduction; cell biology; developmental genetics; gene expression; regeneration; stem cells

DESCRIPTION (provided by applicant): Stem cells are defined as multipotent, self-renewing undifferentiated cells capable of differentiating into numerous cell lineages. A great deal of interest has focused on the potential therapeutic application of such stem cell populations, not only to repair or replace damaged tissues, but as model systems to understand basic developmental processes leading to normal and abnormal cellular development. As a result, this application requests support from the IDeA program to establish a COBRE in Stem Cell Biology and Regenerative Medicine and involves the collaborative interests of a group of four (non-RO1 funded) junior investigators, including 1 from The Jackson Laboratory, under the mentorship of an established investigator in the field of Stem Cell Biology within the Center for Regenerative Medicine at the Maine Medical Center. The intent of including The Jackson Laboratory is to establish and bridge long-term collaborative research programs in the state of Maine. The theme of the application involves investigations into the signals, receptors, signaling cascades, and transcriptional regulatory circuits that control stem cell development. The motives for this application include the use of this award to (i) support, develop, and advance promising young investigators in the field of Stem Cell Biology, (ii) expand existing institutional resources to create a critical mass of mechanism-oriented junior and senior investigators in this area, (iii) establish and expand core resources in Stem Cell Isolation, Pathology, and Molecular Genetics to support ongoing and future investigator needs, and (iv) provide resources requisite for the establishment of an internationally recognized non-profit research institution in the broad area of Stem Cell Biology, with the potential to ultimately apply this information to the field of Regenerative Medicine. The application is comprised of four projects that were chosen in the field of Stem Cell Biology and are the crux of current investigations in the field. These projects include (i) The role of pMesogenin in the mesodermal differentiation of embryonic stem cells, (ii) Notch signaling events in the self-renewal of embryonic stem cells, (iii) The role of Wnt signaling in the preimplantation embryo, and (iv) VEGFR-Notch-Prox resolution of lymph vessel fate. Program expansion beginning in 2003 will include the recruitment of at least 5 investigators with genetic, cellular, and molecular expertise to address mechanistic questions in the general areas of (i) stem cell plasticity, (ii) Hedgehog signaling events in lineage specification, (iii) computational biology, (iv) non-murine models of development, (v) transplantation biology, and (vi) genomics, proteomics, and systems biology. It is anticipated that this award will not only enable the Center for Regenerative Medicine to achieve its goal at a more rapid and efficient rate, but the insight derived from these investigations may significantly impact the field of Stem Cell Biology and Regenerative Medicine.

描述（由申请人提供）： 干细胞是一种多能、自我更新的未分化细胞，能够分化为多种细胞谱系。 大量的兴趣集中在这些干细胞群的潜在治疗应用上，不仅用于修复或替换受损组织，而且作为模型系统来理解导致正常和异常细胞发育的基本发育过程。 因此，本申请请求IDEA计划的支持，以建立干细胞生物学和再生医学的COBRE，并涉及一组四名（非RO 1资助）初级研究人员的合作利益，其中包括来自杰克逊实验室的一名研究人员，在缅因州医学中心再生医学中心干细胞生物学领域的既定研究人员的指导下。 包括杰克逊实验室的目的是建立和桥梁长期合作研究计划在缅因州。 申请的主题涉及到信号，受体，信号级联和转录调控电路控制干细胞发育的调查。 该申请的动机包括使用该奖项（i）支持，发展和推进干细胞生物学领域有前途的年轻研究人员，（ii）扩大现有的机构资源，以创建该领域面向机制的初级和高级研究人员的临界质量，（iii）建立和扩大干细胞分离，病理学，和分子遗传学，以支持正在进行的和未来的研究人员的需求，和（iv）提供必要的资源，建立一个国际公认的非营利研究机构在干细胞生物学的广泛领域，有可能最终将这些信息应用到再生医学领域。 该应用程序由在干细胞生物学领域选择的四个项目组成，是该领域当前调查的关键。 这些项目包括（i）pMesogenin在胚胎干细胞中胚层分化中的作用，（ii）Notch信号传导在胚胎干细胞自我更新中的作用，（iii）Wnt信号传导在植入前胚胎中的作用，以及（iv）VEGFR-Notch-Prox对淋巴管命运的解析。 从2003年开始，该计划将包括招募至少5名具有遗传、细胞和分子专业知识的研究人员，以解决以下一般领域的机制问题：（i）干细胞可塑性，（ii）谱系规范中的Hedgehog信号事件，（iii）计算生物学，（iv）非鼠发育模型，（v）移植生物学，以及（vi）基因组学、蛋白质组学，和系统生物学。 预计该奖项不仅将使再生医学中心能够以更快，更有效的速度实现其目标，而且从这些研究中获得的见解可能会对干细胞生物学和再生医学领域产生重大影响。