Biomimetic Synthesis of an Enamel-Like Material

类牙釉质材料的仿生合成

• 批准号: 6687159
• 负责人: Stefan Friedrich Habelitz
• 金额: $ 18.43万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-04 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6687159
• 关键词: amelogenin; apatites; atomic force microscopy; bioengineering /biomedical engineering; biomaterial development /preparation; biomimetics; chemical structure function; chymotrypsin; crystallization; dental materials; medical implant science; molecular chaperones; protein protein interaction; recombinant DNA; tooth enamel

DESCRIPTION (provided by applicant): Recent advances in biotechnology made proteins and proteases of the extra cellular matrix of enamel available and facilitate the study of their function in-vitro as well as allowing the first attempts to synthesize this calcified tissue in an artificial environment. The overall objective of the current proposal is to determine the physical-chemical conditions required for a protein-guided development of a material that is comparable to the composition, structure and properties of human enamel. This work should lead to an improved understanding of how amelogenin proteins, the spliced variant LRAP+ and two proteases function in the biomineralization process on an ionic and molecular level and how this information can be used to synthesize enamel in-vitro. The hypothesis to be tested is that a biomimetic synthesis of thin layers of an enamel-like material, including composition, microstructure and properties is feasible by the use of full-length and spliced human amelogenins, when combined with selected proteases in mineralizing solutions at specific physical-chemical conditions. This hypothesis will be tested by the following three specific aims: (1) To determine the physical-chemical conditions that promote biomineralization mediated by full-length amelogenin and the spliced variant LRAP+; (2) To determine if amelogenin-induced biomineralization depends on the crystallographic orientation of the apatite substrate; and (3) To synthesize an enamel-like material by gradually degrading the amelogenin matrix using recombinant MMP-20 and serine proteinase while mineralization proceeds.

描述（由申请人提供）：生物技术的最新进展使搪瓷的额外细胞基质的蛋白质和蛋白酶可用，并促进了其功能的研究，并允许首次尝试在人工环境中合成这种钙化组织。 当前建议的总体目的是确定蛋白质引导的材料开发所需的物理化学条件，该材料与人搪瓷的组成，结构和特性相当。 这项工作应提高人们对氨基蛋白蛋白，剪接变体LRAP+和两个蛋白酶如何在离子和分子水平上起作用的蛋白质蛋白，以及如何使用该信息来合成搪瓷含量。 要检验的假设是，当在特定的物理条件下，在矿物质化溶液中与所选蛋白酶结合时，使用全长和剪接的人氨基蛋白的薄层（包括成分，微观结构和特性）是可行的。 该假设将通过以下三个特定目的进行检验：（1）确定促进由全长氨基蛋白和剪接变体LRAP+介导的生物矿化的物理化学条件； （2）确定氨基蛋白素诱导的生物矿化是否取决于磷灰石底物的晶体学取向； （3）通过使用重组MMP-20和丝氨酸蛋白酶在矿化过程中逐渐降解氨基蛋白蛋白基质来合成牙釉质样材料。