Proteomic Biomarkers of Health Behaviors

描述(由申请人提供)： 在评估健康行为对疾病风险的影响时，流行病学研究面临着准确评估个人健康行为的方法学挑战。健康行为评估中的测量误差很常见，不仅会在数量上扭曲分析结果，而且会在质量上扭曲分析结果。 健康行为的生物标记物是能够在观察性研究中进行客观暴露评估的有用工具。我们建议进行一项研究，以建立健康行为的生物标记物，长期目标是识别血清蛋白质谱，客观地反映个人健康行为的水平(例如，定期锻炼、高水果和蔬菜摄入量的饮食)。具体地说，该项目有以下目标：(1)使用MALDI(基质辅助激光解吸和电离)质谱仪技术，测量在为期一年的随机运动干预试验的基线、3个月和12个月收集的血清样本中的蛋白质图谱；(2)确定与运动干预和坚持措施相关的蛋白质图谱的变化；(3)比较不同数据分析方法在追求目标(2)方面的有效性；以及(4)从生物学角度确定常规运动的可能生物标志物。 这些目标将利用现有的随机一年运动干预试验(首席调查者：Anne McTiernan，R01 CA77572)的参与者样本来实现，共有202名参与者(100名女性，102名男性)。这些目标的实现将验证MALDI实验室技术和我们通过C节描述的前期工作开发的数据分析方法，并提供基于MALDI的蛋白质图谱，作为用于流行病学研究的常规运动水平的候选生物标记物。

DESCRIPTION (provided by applicant): In evaluating the effects of health behaviors on disease risks, epidemiologic studies face a methodological challenge of assessing individuals' health behaviors accurately. Measurement errors in health behavior assessments are common and could distort analysis results not only quantitatively, but also qualitatively. Biomarkers of health behaviors are useful tools that enable objective exposure assessments in observational studies. We propose a study for establishing biomarkers of health behaviors with a long-term goal of identifying serum protein profiles that objectively indicate the levels of individuals' healthy behaviors (e.g., regular exercise, diet with high intake of fruits and vegetables). Specifically, this project has the following goals: (1) Using MALDI (Matrix Assisted Laser Disorption and Ionization) mass spectrometry technology, measure protein profiles in serum samples, collected at baseline, 3 months, and 12 months of a yearlong randomized exercise-intervention trial; (2) Identify changes in protein profiles that are associated with the exercise intervention and adherence measures; (3) Compare the effectiveness of different data-analytic approaches in the pursuit of Goal (2); and (4) Biologically identify possible biomarkers of regular exercise. These goals will be accomplished utilizing participant samples from an existing randomized one-year exercise-intervention trial (Principal Investigator: Anne McTiernan, R01 CA77572), with 202 participants (100 women, 102 men). The accomplishment of these goals will validate both the MALDI laboratory techniques and the data-analytic methods we have developed through the preliminary work described in Section C, and provide a MALDI-based protein profile as a candidate biomarker of regular exercise levels for use in epidemiologic research.