Proteomic Biomarkers of Health Behaviors

健康行为的蛋白质组生物标志物

• 批准号: 6887382
• 负责人: PAUL D. LAMPE
• 金额: $ 8.34万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-19 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6887382
• 关键词: behavioral /social science research tag; biomarker; blood chemistry; blood proteins; clinical research; disease /disorder prevention /control; exercise; health behavior; human subject; human therapy evaluation; lifestyle; longitudinal human study; matrix assisted laser desorption ionization; microarray technology; outcomes research; protein sequence; proteomics; psychometrics; spectrometry; statistics /biometry; therapy compliance

DESCRIPTION (provided by applicant): In evaluating the effects of health behaviors on disease risks, epidemiologic studies face a methodological challenge of assessing individuals' health behaviors accurately. Measurement errors in health behavior assessments are common and could distort analysis results not only quantitatively, but also qualitatively. Biomarkers of health behaviors are useful tools that enable objective exposure assessments in observational studies. We propose a study for establishing biomarkers of health behaviors with a long-term goal of identifying serum protein profiles that objectively indicate the levels of individuals' healthy behaviors (e.g., regular exercise, diet with high intake of fruits and vegetables). Specifically, this project has the following goals: (1) Using MALDI (Matrix Assisted Laser Disorption and Ionization) mass spectrometry technology, measure protein profiles in serum samples, collected at baseline, 3 months, and 12 months of a yearlong randomized exercise-intervention trial; (2) Identify changes in protein profiles that are associated with the exercise intervention and adherence measures; (3) Compare the effectiveness of different data-analytic approaches in the pursuit of Goal (2); and (4) Biologically identify possible biomarkers of regular exercise. These goals will be accomplished utilizing participant samples from an existing randomized one-year exercise-intervention trial (Principal Investigator: Anne McTiernan, R01 CA77572), with 202 participants (100 women, 102 men). The accomplishment of these goals will validate both the MALDI laboratory techniques and the data-analytic methods we have developed through the preliminary work described in Section C, and provide a MALDI-based protein profile as a candidate biomarker of regular exercise levels for use in epidemiologic research.

描述（由申请人提供）： 在评估健康行为对疾病风险的影响时，流行病学研究面临着准确评估个人健康行为的方法学挑战。健康行为评估中的测量误差是常见的，不仅会在定量上，而且会在定性上扭曲分析结果。 健康行为的生物标志物是在观察性研究中进行客观暴露评估的有用工具。我们提出了一项建立健康行为生物标志物的研究，其长期目标是鉴定血清蛋白质谱，这些蛋白质谱客观地指示个体健康行为的水平（例如，经常运动，多吃水果和蔬菜）。具体来说，本项目有以下目标：（1）使用MALDI（基质辅助激光解吸电离）质谱技术，测量在基线、3个月和12个月的为期一年的随机运动干预试验中收集的血清样品中的蛋白质谱;（2）确定与运动干预和坚持措施相关的蛋白质谱的变化;（3）比较不同数据分析方法在追求目标（2）方面的有效性;（4）从生物学上确定定期锻炼的可能生物标志物。 这些目标将利用现有的一项为期一年的随机运动干预试验（主要研究者：Anne McTiernan，R 01 CA 77572）的受试者样本来实现，该试验共有202名受试者（100名女性，102名男性）。这些目标的实现将验证MALDI实验室技术和数据分析方法，我们已经通过C节中描述的初步工作开发，并提供了一个基于MALDI的蛋白质谱作为候选生物标志物的定期运动水平用于流行病学研究。

项目成果

A cautionary note on the evaluation of biomarkers of subtypes of a single disease.

关于评估单一疾病亚型生物标志物的警告。

• DOI: 10.1093/aje/kwn183
• 发表时间: 2008
• 期刊: American journal of epidemiology
• 影响因子: 5
• 作者: Adewale,AdeniyiJ;Liu,Qi;Dinu,Irina;Lampe,PaulD;Mitchell,BreeL;Yasui,Yutaka
• 通讯作者: Yasui,Yutaka

Biochips that sequentially capture and focus antigens for immunoaffinity MALDI-TOF MS: a new tool for biomarker verification.

• DOI: 10.1002/pmic.201000219
• 发表时间: 2010-11
• 期刊: PROTEOMICS
• 影响因子: 3.4
• 作者: Brauer, Heather Ann;Lampe, Paul D.;Yasui, Yutaka Y.;Hamajima, Nobuyuki;Stolowitz, Mark L.
• 通讯作者: Stolowitz, Mark L.