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Comparison of US and European Babesia divergens Isolates

美国和欧洲分歧巴贝斯虫分离株的比较

基本信息

批准号：
6725465
负责人：
PATRICIA J HOLMAN
金额：
$ 7.28万
依托单位：
TEXAS A&M UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-04-01 至 2006-03-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by the applicant): Babesia divergens is a tick transmitted hemoprotozoan parasite that causes human Babesiosis in Europe. Neither B. divergens nor its vector tick are indigenous to the U.S. However, a recent case of acute Babesiosis in a resident of Kentucky was shown to be caused by a parasite morphologically indistinguishable from European Babesia divergens. The parasite in the Kentucky case was found to differ in the small subunit ribosomal RNA gene sequence at only 3 base positions from that of European B. divergens, providing evidence of conspecifity. Recently a Babesia isolate from cottontail rabbits on Nantucket Island was reported to have an identical gene sequence to that of the Kentucky parasite. Molecular epidemiology has also identified this parasite in Ixodes ticks on Nantucket Island, suggesting that an enzootic situation exists. The identification of another Babesia sp. in the US capable of causing human babesiosis is of major concern. Two other Babesia species that cause human Babesiosis in the US, Babesia microti, occurring mostly in the northeastern U.S., and the WA1 isolate, occurring in Washington state and California, have been implicated in blood transfusion acquired cases of babesiosis. Thus, the finding that another previously unknown human blood pathogen is troublesome. The purpose of this study is to compare the culture requirements and target genes of the Kentucky and Nantucket Babesia isolates with European B. divergens (Purnell strain) to determine if conspecificity exists among these parasites. Specific aims include establishing in vitro cultures of the Nantucket isolate and molecular comparison of ITS, HSP70, and cysteine protease genes from European B. divergens with those of the Kentucky and Nantucket isolates. Cultures of the Nantucket isolate will provide an unlimited source of parasites to complete these goals and for future studies. The molecular aspects may provide information that will be useful in future efforts to devise diagnostic tests.

描述(由申请人提供)：发端巴贝斯虫是一种由壁虱传播的血液原虫寄生虫，在欧洲引起人类巴贝斯虫病。然而，最近肯塔基州一名居民的急性巴贝斯虫病病例被证明是由一种寄生虫引起的，这种寄生虫在形态上与欧洲的巴贝斯虫难以区分。肯塔基州病例中的寄生虫被发现在小亚基核糖体RNA基因序列上与欧洲分枝杆菌的小亚单位核糖体RNA基因序列只有3个碱基不同，这为同源性提供了证据。最近，据报道，从楠塔基特岛上的棉尾兔身上分离到的巴贝斯虫与肯塔基州寄生虫具有相同的基因序列。分子流行病学也在楠塔基特岛的硬蜱中发现了这种寄生虫，这表明存在地方性疫情。另一株巴贝斯虫的鉴定。在美国，能够引起人类巴贝斯虫病的是主要令人担忧的问题。另外两种在美国引起人类巴贝斯虫病的巴贝斯虫物种--主要发生在美国东北部的微型巴贝斯虫，以及发生在华盛顿州和加利福尼亚州的WA1分离株，都与输血获得性巴贝斯虫病病例有关。因此，发现另一种以前不为人知的人类血液病原体是很麻烦的。本研究的目的是比较肯塔基州巴贝斯虫和南塔基特巴贝斯虫分离物与欧洲巴贝斯伯克斯菌(Purnell株)的培养要求和靶基因，以确定这些寄生虫之间是否存在一致性。具体目标包括建立南塔基特分离株的体外培养，以及欧洲分枝杆菌ITS、HSP70和半胱氨酸蛋白酶基因与肯塔基州和南塔基特分离株的分子比较。楠塔基特分离株的培养将为完成这些目标和未来的研究提供无限的寄生虫来源。分子方面可能会提供对未来设计诊断测试有用的信息。