Analysis of novel non coding mRNA-like RNAs in mice.

描述(由申请人提供)： 通过实验方法，在蠕虫、植物、昆虫和哺乳动物中发现了大量新的非蛋白质编码RNA(NcRNAs)。然而，随着大规模基因组测序的出现，特别是在人类和小鼠中，确实已经识别了数百个未知分类和功能的额外ncRNA。仅在小鼠中，ncRNAs被认为代表了基因组中所有转录单位的大约三分之一。由于过去的研究主要集中在蛋白质编码基因和蛋白质组上，因此对ncRNA的功能仍然知之甚少。为了弥合对这些类型转录单位的认识差距，首先，将基于生物信息学对RIKEN-FANTOM鉴定的小鼠假定的非编码mRNA样RNA进行筛选，以确定新的候选功能研究；其次，将对一组选定的ncRNAs进行分子表达分析，以确定组织特异性和/或大量表达的转录本。在分子分析之后，一组精选的非编码RNA将通过小鼠的功能丧失遗传学进行分析。

DESCRIPTION (provided by applicant): Large numbers of novel non-protein-coding RNAs (ncRNAs) have been characterized in worms, plants, insects, and mammals by experimental approaches. However, with the advent of large-scale genome sequencing, especially in humans and mice, literally hundreds of additional ncRNAs of unknown classification and function have been identified. In mice alone, the ncRNAs are thought to represent approximately 1/3 of all transcription units in the genome. ncRNA function remains poorly understood since past research has focused primarily on protein coding genes and the proteome. To begin to close the knowledge gap on these types of transcription units, firstly, bioinformatics-based filtering of mouse putative non coding mRNA-like RNAs identified by RIKEN-FANTOM will be conducted to identify novel candidates for functional studies Secondly, molecular expression analysis of a selected set of ncRNAs will be conducted to identify tissue specific and/or abundantly expressed transcripts. Following from the molecular analysis, a select group of non-coding RNAs will be analyzed by loss-of-function genetics in mice.