Analysis of novel non coding mRNA-like RNAs in mice

描述（由申请人提供）： 大量的新的非蛋白质编码RNA（ncRNA）已经通过实验方法在蠕虫，植物，昆虫和哺乳动物中进行了表征。然而，随着大规模基因组测序的出现，特别是在人类和小鼠中，已经鉴定出数百种分类和功能未知的额外ncRNA。仅在小鼠中，ncRNA被认为代表基因组中所有转录单位的约1/3。 由于过去的研究主要集中在蛋白质编码基因和蛋白质组，因此对ncRNA的功能仍然知之甚少。为了开始缩小这些类型转录单位的知识差距，首先，将对RIKEN-FANTOM鉴定的小鼠推定非编码mRNA样RNA进行基于生物信息学的过滤，以鉴定用于功能研究的新候选物。其次，将对选定的一组ncRNA进行分子表达分析，以鉴定组织特异性和/或大量表达的转录物。在分子分析之后，将通过小鼠中的功能丧失遗传学分析选定的一组非编码RNA。

DESCRIPTION (provided by applicant): Large numbers of novel non-protein-coding RNAs (ncRNAs) have been characterized in worms, plants, insects, and mammals by experimental approaches. However, with the advent of large-scale genome sequencing, especially in humans and mice, literally hundreds of additional ncRNAs of unknown classification and function have been identified. In mice alone, the ncRNAs are thought to represent approximately 1/3 of all transcription units in the genome. ncRNA function remains poorly understood since past research has focused primarily on protein coding genes and the proteome. To begin to close the knowledge gap on these types of transcription units, firstly, bioinformatics-based filtering of mouse putative non coding mRNA-like RNAs identified by RIKEN-FANTOM will be conducted to identify novel candidates for functional studies Secondly, molecular expression analysis of a selected set of ncRNAs will be conducted to identify tissue specific and/or abundantly expressed transcripts. Following from the molecular analysis, a select group of non-coding RNAs will be analyzed by loss-of-function genetics in mice.