Flow Cytometry Resource Facility

摘要，流式细胞仪资源设施(FCRF) 印第安纳大学西蒙癌症中心(IUSCC)的流式细胞术资源设施(FCRF)支持 癌症中心成员的研究努力。流式细胞仪服务是不可或缺的关键服务 为中心所有项目中的大量IUSCC成员的研究活动提供支持。访问 全面的流式细胞术应用，例如批量和作为单个细胞的细胞分选， 免疫表型分析，细胞周期分析，以及单细胞水平的基因组和代谢调节，是 癌症研究的无价资源。提供一系列流式细胞仪技术的集中式核心 与单独购买这些工具相比，提供了几个优势。此外，FCRF 为IUSCC成员提供最先进的服务，成本大大降低，使其更简单、更高效 通过共享设施提供这些服务。该委员会的基础设施和组织安排 FCRF已经很好地建立了，因为这个设施已经在密歇根大学医学院的校园里超过了 三十年，自以来一直由核心导演领导。 在FCRF中，包括最近收购的CyTOF2，是自2014年初以来增加的。FCRF核心提出了两项建议 具体目标：1)提供卓越、一致、及时和经济的多色分析、细胞分选、 对所有IUSCC成员进行图像流动分析、单细胞基因组学和蛋白质组分析，以促进其 以癌症为重点的研究；以及2)提供专门知识，通过以下方式提高IUSCC成员的生产力 就调查如何最好地利用集中化和标准化的 性价比高的流式细胞仪等相关服务。这包括交换协议和结果 在调查人员和推进科学知识的传播之间。通过机构和IUSCC 在支持下，FCRF继续提供尖端能力，以促进IUSCC的开创性研究 并允许他们将先进技术应用于他们的研究。作为共享的核心设施 FCRF拥有先进的技术，对许多IUSCC成员来说是一种经济和必要的资源。

ABSTRACT, Flow Cytometry Resource Facility (FCRF) The Flow Cytometry Resource Facility (FCRF) of the Indiana University Simon Cancer Center (IUSCC) supports the research efforts of members of the Cancer Center. Flow cytometric services are integral and critical services for the research activities of a large number of IUSCC members in all programs of the Center. Access to a comprehensive array of flow cytometric applications such as cell sorting both in bulk and as single cells, immunophenotypic analysis, cell cycle analysis, and genomic and metabolic regulation at the single cell level, is an invaluable resource for cancer research. A centralized core offering an array of flow cytometric technologies provides several advantages over the acquisition of these tools on an individual basis. Furthermore, the FCRF offers state-of-the-art services at a much-reduced cost to IUSCC members making it simpler and more efficient to provide these services through a shared facility. The infrastructure and organizational arrangements of the FCRF are well established as this facility has been on the campus of the IU School of Medicine for more than three decades and has been led by the core director the since 1989. Eight of the 11 instruments currently housed in the FCRF, including a recently acquired CyTOF2, were added since early 2014. The FCRF core proposes two specific aims: 1) to provide outstanding, consistent, timely, and economical multicolor analysis, cell sorting, image flow analysis, and single cell genomics and proteomic analysis to all IUSCC members to facilitate their cancer-focused research; and 2) to provide expertise to enhance the productivity of IUSCC members by providing advice and consultations on how investigations can best make use of the centralized and standardized cost-effective flow cytometric and other related services. This includes the exchange of protocols and results between investigators and advancing the distribution of scientific knowledge. Through institutional and IUSCC support, the FCRF continues to provide cutting-edge capabilities to promote pioneering research by IUSCC members and allow them to apply advanced technologies to their research. As a shared core facility with advanced technologies, the FCRF is an economic and essential resource to many IUSCC members.