Novel plant-based Antimicrobials against oral pathogens

针对口腔病原体的新型植物抗菌剂

• 批准号: 6833726
• 负责人: Chifu Brad Huang
• 金额: $ 10万
• 依托单位: ORACEUTICALS, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6833726
• 关键词: antiinfective agents; beta galactosidase; biotechnology; functional /structural genomics; growth media; high throughput technology; oral bacteria; oral health; plant extracts; plant genetics; recombinant DNA; reporter genes; technology /technique development; tissue /cell culture

DESCRIPTION: A novel platform biotechnology, "natural products genomics" for seeking plant-based medicines has been developed which utilizes high throughput screening to identify active compounds in large populations of mutant plant cell cultures. This technology likely accesses the entire range of metabolic products of which the plant is capable. In this regard, plants, particularly their roots, are constantly under attack from bacterial and fungal pathogens that share similarities with those that infect the human oral cavity. The defense mechanisms of plants are therefore a potential source of antimicrobial compounds that may be of value in oral health. This application is to develop methods that will allow the novel platform technology to be applied to the identification and separation of plant compounds that are active against oral pathogens. For this, it is first necessary to develop an ultra high-throughput screen for antimicrobial activity that can be used with very small plant cell cultures. The development of this screen represents the deliverables for Phase I of this STTR. There are two major aspects to this development. First, appropriate pathogen cultures must be developed in which an indicator of cell damage/killing can be used to evaluate efficacy of plant culture secretions. Second, a mechanism for exposing the pathogen cultures to the mutant plant cell cultures must be developed. The application is to explore the possibility that pathogen and plant cultures can be grown on "matching" 96 well plates as co-culture systems, with plant cultures grown in wells with a semi-permeable membrane. The plates can then be placed in contact for 24h, with culture media separated only by the semi-permeable membrane, allowing secreted products from the cultures to diffuse onto the pathogens. If successful, this STTR will represent an extremely valuable transfer of the natural products genomics technology into the field of oral health medications. Compounds discovered by this method should be of considerable economic and medical value as proprietary medicines for the control of oral pathogens.

产品说明：一种新的平台生物技术，“天然产物基因组学”，用于寻找植物为基础的药物已经开发出来，它利用高通量筛选，以确定活性化合物在大量的突变植物细胞培养。这项技术可能会获得植物能够产生的全部代谢产物。在这方面，植物，特别是它们的根，经常受到与感染人类口腔的病原体相似的细菌和真菌病原体的攻击。因此，植物的防御机制是可能对口腔健康有价值的抗菌化合物的潜在来源。该应用旨在开发方法，使新型平台技术能够应用于识别和分离对口腔病原体有活性的植物化合物。为此，首先有必要开发一种可用于非常小的植物细胞培养物的超高通量抗微生物活性筛选。该屏幕的开发代表了本STTR第I阶段的可交付成果。这一发展有两个主要方面。首先，必须开发合适的病原体培养物，其中细胞损伤/杀伤的指标可用于评估植物培养物分泌物的功效。其次，必须开发一种将病原体培养物暴露于突变体植物细胞培养物的机制。该应用是为了探索病原体和植物培养物可以在“匹配的”96孔板上生长作为共培养系统的可能性，其中植物培养物在具有半透膜的威尔斯孔中生长。然后可以将平板与培养基接触24小时，培养基仅通过半透膜分离，允许来自培养物的分泌产物扩散到病原体上。如果成功的话，这个STTR将代表着天然产物基因组学技术向口腔健康药物领域的一个非常有价值的转移。通过这种方法发现的化合物作为控制口腔病原体的专利药物应该具有相当大的经济和医学价值。