Human Embryonic Stem Cell Toolbox Workshop

描述（由申请人提供）：“人类胚胎干细胞培养（HEST）”研讨会的总体目标是提供详细的“动手”培训，以提高参与者未来在人类胚胎干细胞（hES）实验中获得更统一分析和结果的成功。不能直接从方案和/或教科书教导或理解hES细胞的扩增和分化。技术或“工具”正在发展，需要与受过培训的个人进行一对一的演示和讨论。HEST将汇集在胚胎学，发育生物学，组织工程和细胞生物学方面受过特殊训练的科学家，以学习hES细胞自我更新的复杂细节，“如何”和理论。研究中心为格鲁吉亚大学，使用NIH批准的细胞系BGN 01，如果需要，使用BresaGen，linc提供的BGN 02 - 04。格鲁吉亚大学与BresaGen，Inc.有着独特而牢固的关系。和它的科学家（有些人在格鲁吉亚大学兼职）。结合这个小组有强大的和不断增长的hES细胞实验室，正在进行维持，分析，诱导定向体外分化和动物模型测试的研究。HEST教师包括七名格鲁吉亚大学员工和六名BresaGen员工。这个项目的一个独特的方面是我们认为，研讨会只是参与者和全职协调员/主要讲师和教师之间的许多培训交流的第一个。持续的指导将通过电话、电子邮件甚至现场访问的形式进行故障排除讨论，此外还将在研讨会后提供关键的起始材料，如测试的饲养细胞和培养基。HEST研讨会将每年举办两次，每次16人参加。一次信息丰富且成功的HEST需要在研讨会之前进行至少六周的hES细胞繁殖和分化。然而，后续的指导可能会更加劳动密集，因此需要一个全职的车间协调员和一个研究生助教。参与者的招募将是全面的，如果格鲁吉亚大学多样性协调员将协助招募代表性不足的科学家参加HEST研讨会。

DESCRIPTION (provided by applicant): The overall goal of the "Human Embryonic Stem cell Toolbox (HEST)" workshop is to provided detailed "hands on" training to enhance the participants' future success in obtaining more uniform analysis and results in their human embryonic stem cells (hES) experiments. Propagating and differentiating hES cells cannot be taught or understood directly from protocols and or textbooks. The techniques or "tools" are evolving and require one on one demonstrations and discussions with trained individuals. HEST will bring together scientists with a particular training in embryology, developmental biology, tissue engineering, and cell biology to learn the intricate details, the "how to" and theory of hES cells self renewal. The site is the University of Georgia using NIH approved cell line BGN01 and if needed BGN02 -04 provided by BresaGen, linc. The University of Georgia has a unique and strong relationship with BresaGen, Inc. and its scientists (some have adjunct appointments with the University of Georgia). Combined this group have strong and growing hES cell laboratories that are conducting research on maintaining, analyzing, inducing directed in vitro differentiation and testing in animal models. The HEST faculty comprises of seven University of Georgia employees and six BresaGen employees. A unique aspect of this project is our view that the workshop is only the first of many training communications between participants and the full time coordinator/main instructor and faculty. The ongoing guidance will be in the form of troubleshooting discussions via phone calls, emails and even on site visits, in addition to supplying critical starting material such as tested feeder cells and culture media after the workshop. The HEST workshop will be conducted twice a year with 16 participants in each. An informative and successful HEST necessitates at least six weeks of hES cells propagation and differentiation prior to the workshop. However the follow up instruction will likely be even more labor intensive, thus requiring a full time workshop coordinator and a graduate student teaching assistant. The recruitment of participants will be comprehensive and a University if Georgia diversity coordinator will assist in recruiting underrepresented scientists to the HEST workshop.