Human Embryonic Stem Cell Toolbox Workshop

描述(申请人提供)：“人类胚胎干细胞工具箱(HEST)”工作坊的总体目标是提供详细的“动手”培训，以加强参与者未来在其人类胚胎干细胞(HES)实验中获得更统一的分析和结果方面的成功。HES细胞的繁殖和分化不能直接从协议和/或教科书中传授或理解。技术或“工具”正在发展，需要一对一的演示和与训练有素的个人进行讨论。Hest将召集在胚胎学、发育生物学、组织工程学和细胞生物学方面接受过特殊培训的科学家，学习HES细胞自我更新的复杂细节、“如何”和理论。该网站是佐治亚大学使用NIH批准的细胞系BGN01，如果需要，BGN02-04由Bresagen，Linc提供。佐治亚大学与Bresgen，Inc.及其科学家有着独特而牢固的关系(其中一些人与佐治亚大学有兼职)。合并后，该集团拥有强大的和不断增长的HES细胞实验室，这些实验室正在进行维持、分析、诱导定向体外分化的研究，并在动物模型中进行测试。Hest的教职员工包括佐治亚大学的七名员工和布雷斯根大学的六名员工。这个项目的一个独特方面是我们认为，研讨会只是参与者与全职协调员/主要讲师和教职员工之间许多培训交流的第一次。正在进行的指导将以通过电话、电子邮件甚至现场访问进行故障排除讨论的形式进行，此外还将在研讨会结束后提供关键的启动材料，如测试的饲养层细胞和培养介质。HEST研讨会将每年举办两次，每期16人。信息丰富且成功的HEST需要在研讨会之前进行至少六周的HES细胞增殖和分化。然而，后续的指导可能会更加劳动密集型，因此需要一名全职研讨会协调员和一名研究生助教。参与者的招募将是全面的，一名佐治亚州大学多样性协调员将协助招募代表人数不足的科学家参加HEST讲习班。

DESCRIPTION (provided by applicant): The overall goal of the "Human Embryonic Stem cell Toolbox (HEST)" workshop is to provided detailed "hands on" training to enhance the participants' future success in obtaining more uniform analysis and results in their human embryonic stem cells (hES) experiments. Propagating and differentiating hES cells cannot be taught or understood directly from protocols and or textbooks. The techniques or "tools" are evolving and require one on one demonstrations and discussions with trained individuals. HEST will bring together scientists with a particular training in embryology, developmental biology, tissue engineering, and cell biology to learn the intricate details, the "how to" and theory of hES cells self renewal. The site is the University of Georgia using NIH approved cell line BGN01 and if needed BGN02 -04 provided by BresaGen, linc. The University of Georgia has a unique and strong relationship with BresaGen, Inc. and its scientists (some have adjunct appointments with the University of Georgia). Combined this group have strong and growing hES cell laboratories that are conducting research on maintaining, analyzing, inducing directed in vitro differentiation and testing in animal models. The HEST faculty comprises of seven University of Georgia employees and six BresaGen employees. A unique aspect of this project is our view that the workshop is only the first of many training communications between participants and the full time coordinator/main instructor and faculty. The ongoing guidance will be in the form of troubleshooting discussions via phone calls, emails and even on site visits, in addition to supplying critical starting material such as tested feeder cells and culture media after the workshop. The HEST workshop will be conducted twice a year with 16 participants in each. An informative and successful HEST necessitates at least six weeks of hES cells propagation and differentiation prior to the workshop. However the follow up instruction will likely be even more labor intensive, thus requiring a full time workshop coordinator and a graduate student teaching assistant. The recruitment of participants will be comprehensive and a University if Georgia diversity coordinator will assist in recruiting underrepresented scientists to the HEST workshop.