Human Embryonic Stem Cell Toolbox Workshop

人类胚胎干细胞工具箱工作坊

• 批准号: 6747359
• 负责人: STEVEN L STICE
• 金额: $ 16.2万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-12 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6747359
• 关键词: Human; Embryonic; Stem; Cell; Toolbox

DESCRIPTION (provided by applicant): The overall goal of the "Human Embryonic Stem cell Toolbox (HEST)" workshop is to provided detailed "hands on" training to enhance the participants' future success in obtaining more uniform analysis and results in their human embryonic stem cells (hES) experiments. Propagating and differentiating hES cells cannot be taught or understood directly from protocols and or textbooks. The techniques or "tools" are evolving and require one on one demonstrations and discussions with trained individuals. HEST will bring together scientists with a particular training in embryology, developmental biology, tissue engineering, and cell biology to learn the intricate details, the "how to" and theory of hES cells self renewal. The site is the University of Georgia using NIH approved cell line BGN01 and if needed BGN02 -04 provided by BresaGen, linc. The University of Georgia has a unique and strong relationship with BresaGen, Inc. and its scientists (some have adjunct appointments with the University of Georgia). Combined this group have strong and growing hES cell laboratories that are conducting research on maintaining, analyzing, inducing directed in vitro differentiation and testing in animal models. The HEST faculty comprises of seven University of Georgia employees and six BresaGen employees. A unique aspect of this project is our view that the workshop is only the first of many training communications between participants and the full time coordinator/main instructor and faculty. The ongoing guidance will be in the form of troubleshooting discussions via phone calls, emails and even on site visits, in addition to supplying critical starting material such as tested feeder cells and culture media after the workshop. The HEST workshop will be conducted twice a year with 16 participants in each. An informative and successful HEST necessitates at least six weeks of hES cells propagation and differentiation prior to the workshop. However the follow up instruction will likely be even more labor intensive, thus requiring a full time workshop coordinator and a graduate student teaching assistant. The recruitment of participants will be comprehensive and a University if Georgia diversity coordinator will assist in recruiting underrepresented scientists to the HEST workshop.

描述（由申请人提供）：“人类胚胎干细胞工具箱（HEST）”研讨会的总体目标是提供详细的“实践”培训，以提高参与者未来在人类胚胎干细胞（hES）实验中获得更统一的分析和结果的成功。人类胚胎干细胞的繁殖和分化不能直接从协议或教科书中教授或理解。技术或“工具”正在发展，需要一对一的演示，并与受过培训的个人进行讨论。HEST将汇集在胚胎学、发育生物学、组织工程学和细胞生物学方面受过特殊训练的科学家，学习he细胞自我更新的复杂细节、“如何”和理论。该网站是佐治亚大学使用美国国立卫生研究院批准的细胞系BGN01，如果需要的话，BGN02 -04由BresaGen， linc提供。佐治亚大学与BresaGen， Inc.及其科学家有着独特而牢固的关系（其中一些与佐治亚大学有兼职关系）。该小组拥有强大且不断发展的hES细胞实验室，正在进行维持、分析、诱导定向体外分化和动物模型测试的研究。HEST的教师由七名佐治亚大学的员工和六名布雷萨根的员工组成。这个项目的一个独特之处在于，我们认为研讨会只是参与者与全职协调员/主要讲师和教师之间许多培训交流的第一次。除了在研讨会结束后提供关键的起始材料，如经过测试的饲养细胞和培养基外，正在进行的指导将以通过电话、电子邮件甚至现场访问进行故障排除讨论的形式进行。无害环境技术讲习班每年举办两次，每次16人参加。在研讨会之前，一个信息丰富且成功的HEST需要至少六周的he细胞增殖和分化。然而，后续的指导可能会更加劳动密集，因此需要一个全职的研讨会协调员和一个研究生助教。参加者的征聘将是全面的，格鲁吉亚大学的多样性协调员将协助征聘代表性不足的科学家参加无害环境技术讲习班。