Human Embryonic Stem Cell Toolbox Workshop

人类胚胎干细胞工具箱工作坊

• 批准号: 6747359
• 负责人: STEVEN L STICE
• 金额: $ 16.2万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-12 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6747359
• 关键词: Human; Embryonic; Stem; Cell; Toolbox

DESCRIPTION (provided by applicant): The overall goal of the "Human Embryonic Stem cell Toolbox (HEST)" workshop is to provided detailed "hands on" training to enhance the participants' future success in obtaining more uniform analysis and results in their human embryonic stem cells (hES) experiments. Propagating and differentiating hES cells cannot be taught or understood directly from protocols and or textbooks. The techniques or "tools" are evolving and require one on one demonstrations and discussions with trained individuals. HEST will bring together scientists with a particular training in embryology, developmental biology, tissue engineering, and cell biology to learn the intricate details, the "how to" and theory of hES cells self renewal. The site is the University of Georgia using NIH approved cell line BGN01 and if needed BGN02 -04 provided by BresaGen, linc. The University of Georgia has a unique and strong relationship with BresaGen, Inc. and its scientists (some have adjunct appointments with the University of Georgia). Combined this group have strong and growing hES cell laboratories that are conducting research on maintaining, analyzing, inducing directed in vitro differentiation and testing in animal models. The HEST faculty comprises of seven University of Georgia employees and six BresaGen employees. A unique aspect of this project is our view that the workshop is only the first of many training communications between participants and the full time coordinator/main instructor and faculty. The ongoing guidance will be in the form of troubleshooting discussions via phone calls, emails and even on site visits, in addition to supplying critical starting material such as tested feeder cells and culture media after the workshop. The HEST workshop will be conducted twice a year with 16 participants in each. An informative and successful HEST necessitates at least six weeks of hES cells propagation and differentiation prior to the workshop. However the follow up instruction will likely be even more labor intensive, thus requiring a full time workshop coordinator and a graduate student teaching assistant. The recruitment of participants will be comprehensive and a University if Georgia diversity coordinator will assist in recruiting underrepresented scientists to the HEST workshop.

描述（由申请人提供）：“人类胚胎干细胞工具箱（HEST）”研讨会的总体目标是提供详细的“动手”培训，以增强参与者在获得更多均匀分析并在其人类胚胎干细胞（HES）实验中取得成功的未来成功。不能直接从协议和或教科书中教授或理解传播和区分的HES细胞。这些技术或“工具”正在发展，需要一对一的演示和与训练有素的人进行讨论。 HEST将通过在胚胎学，发育生物学，组织工程和细胞生物学方面进行特定的培训来汇集科学家，以学习错综复杂的细节，即HES细胞自我更新的“如何”和理论。该地点是佐治亚大学使用NIH批准的细胞系BGN01，如果需要的话，BNES2 -04由Bresagen，Linc提供。佐治亚大学与Bresagen，Inc。及其科学家建立了独特而牢固的关系（有些人与佐治亚大学有辅助任命）。该组的组合具有强大而不断增长的HES细胞实验室，这些实验室正在进行研究，分析，诱导动物模型中定向的体外分化和测试。 Hest教职员工由乔治亚大学七名员工和六名布雷萨根雇员组成。该项目的一个独特方面是，我们认为研讨会只是参与者与全职协调员/主要讲师和教职员工之间的许多培训通信中的第一个。持续的指导将以通过电话，电子邮件甚至现场访问进行故障排除讨论的形式，此外还提供了关键的起始材料，例如讲习班后测试的馈线细胞和培养基。 Hest研讨会每年将进行两次，每位参与者中有16个。一个信息丰富且成功的HEST需要在研讨会之前至少六周的HES细胞传播和分化。但是，后续指导可能会更加密集，因此需要全职研讨会协调员和研究生助教。如果佐治亚州多样性协调员将有助于招募缺乏代表性的科学家参加Hest研讨会，那么参与者的招募将是全面的，并且是大学。