Human Embryonic Stem Cell Toolbox Workshop

人类胚胎干细胞工具箱工作坊

• 批准号: 6747359
• 负责人: STEVEN L STICE
• 金额: $ 16.2万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-12 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6747359
• 关键词: Human; Embryonic; Stem; Cell; Toolbox

DESCRIPTION (provided by applicant): The overall goal of the "Human Embryonic Stem cell Toolbox (HEST)" workshop is to provided detailed "hands on" training to enhance the participants' future success in obtaining more uniform analysis and results in their human embryonic stem cells (hES) experiments. Propagating and differentiating hES cells cannot be taught or understood directly from protocols and or textbooks. The techniques or "tools" are evolving and require one on one demonstrations and discussions with trained individuals. HEST will bring together scientists with a particular training in embryology, developmental biology, tissue engineering, and cell biology to learn the intricate details, the "how to" and theory of hES cells self renewal. The site is the University of Georgia using NIH approved cell line BGN01 and if needed BGN02 -04 provided by BresaGen, linc. The University of Georgia has a unique and strong relationship with BresaGen, Inc. and its scientists (some have adjunct appointments with the University of Georgia). Combined this group have strong and growing hES cell laboratories that are conducting research on maintaining, analyzing, inducing directed in vitro differentiation and testing in animal models. The HEST faculty comprises of seven University of Georgia employees and six BresaGen employees. A unique aspect of this project is our view that the workshop is only the first of many training communications between participants and the full time coordinator/main instructor and faculty. The ongoing guidance will be in the form of troubleshooting discussions via phone calls, emails and even on site visits, in addition to supplying critical starting material such as tested feeder cells and culture media after the workshop. The HEST workshop will be conducted twice a year with 16 participants in each. An informative and successful HEST necessitates at least six weeks of hES cells propagation and differentiation prior to the workshop. However the follow up instruction will likely be even more labor intensive, thus requiring a full time workshop coordinator and a graduate student teaching assistant. The recruitment of participants will be comprehensive and a University if Georgia diversity coordinator will assist in recruiting underrepresented scientists to the HEST workshop.

描述（由申请人提供）：“人类胚胎干细胞工具箱（HEST）”研讨会的总体目标是提供详细的“实践”培训，以提高参与者未来在人类胚胎干细胞（hES）实验中获得更统一的分析和结果的成功率。 hES 细胞的增殖和分化不能直接从实验方案和/或教科书中进行教导或理解。这些技术或“工具”正在不断发展，需要与经过培训的人员进行一对一的演示和讨论。 HEST 将汇集在胚胎学、发育生物学、组织工程和细胞生物学方面接受过特殊培训的科学家，以学习 hES 细胞自我更新的复杂细节、“如何做”和理论。该站点是佐治亚大学，使用 NIH 批准的细胞系 BGN01，如果需要，则使用 BresaGen, linc 提供的 BGN02 -04。佐治亚大学与 BresaGen, Inc. 及其科学家（其中一些科学家与佐治亚大学有兼职）有着独特而牢固的关系。该小组拥有强大且不断发展的 hES 细胞实验室，正在进行维持、分析、诱导体外定向分化和动物模型测试方面的研究。 HEST 教职人员由 7 名佐治亚大学员工和 6 名 BresaGen 员工组成。该项目的一个独特之处是我们认为研讨会只是参与者与全职协调员/主要讲师和教师之间众多培训交流中的第一次。持续的指导将采取通过电话、电子邮件甚至现场访问进行故障排除讨论的形式，此外还提供关键的起始材料，例如经过测试的饲养细胞和培养基。 HEST 研讨会每年举办两次，每次有 16 名参与者。信息丰富且成功的 HEST 需要在研讨会之前进行至少六周的 hES 细胞增殖和分化。然而，后续指导可能会更加劳动密集，因此需要一名全职研讨会协调员和一名研究生助教。参与者的招募将是全面的，如果佐治亚大学多样性协调员将协助招募代表性不足的科学家参加 HEST 研讨会。