SpectruMedix 9610 Mutation Discovery System

描述（由申请人提供）：SpectruMedix SCE9610 Reveal Mutation Discovery Instrument System允许96个平行分析分离，以板的形式进行突变检测和DNA多态性筛选，通过lemporal gradient毛细管电泳（TGCE）。在接近不同放大子熔化温度的温度下，可以分离出随机再退火的同双工和异双工组合。通过比较野生型对照与实验样品的电泳图谱，可以很容易地揭示序列变异阳性。与目前使用的变性高效液相色谱（DHPLC）突变检测技术相比，SCE9610具有明显的优势。首先，与DHPLC系统不同，DHPLC系统配备了单个分离柱，允许一次分析一个样品，SCE9610的分离在单个毛细血管中同时发生。因此，每个毛细管相当于一个单柱色谱仪。SCE9610使用一个CCD检测器，通过柱上激光激发同时记录来自所有毛细血管的荧光信号。这种简单而高效的光学设计提供了一个非常强大的检测系统。最重要的是，SCE9610配置将分析96孔微滴皿所需的时间从目前DHPLC系统的16小时减少到不到2小时。当前DHPLC系统的另一个限制是需要为不同类型的突变建立特定的温度，以获得最佳的突变检测灵敏度。SCE9610系统能够在单次运行中在精确控制的温度梯度下进行样品分析，从而消除了温度优化以最大化检测灵敏度的需要，从而克服了这一限制。大规模多路复用、高分离效率、快速分析时间、全自动和灵敏检测的结合使SCE9610成为北卡罗来纳大学教堂山校区许多项目的高通量应用的非常合适的分析平台。

DESCRIPTION (provided by applicant): The SpectruMedix SCE9610 Reveal Mutation Discovery Instrument System allows for 96 parallel analytical separations in a plate format for mutation detection and DNA polymorphism screening by lemporal gradient capillary electrophoresis (TGCE). At a temperature close to that of a variant amplicon melting temperature, the randomly reannealed combinations of homoduplexes and heteroduplexes can be separated. By comparing the electropherogram patterns of the wild-type control with experimental samples, sequence variation positives can be readily revealed. Compared with the currently used Denatured High Performance Liquid Chromatography (DHPLC) mutation detection technology, the SCE9610 offers distinctive advantages. First, unlike the DHPLC system, which is equipped with a single separation column that allows analysis of one sample at a time, separations on the SCE9610 occur simultaneously in individual capillaries. Each capillary is therefore the equivalent of a singlecolumn chromatography instrument. The SCE9610 uses one CCD detector to simultaneously record Fluorescencesignal from all capillaries with on-column laser excitation. This simple yet highly efficient optical design renders an extremely robust detection system. Most importantly, the SCE9610 configuration reduces the amount of time needed to analyze a 96-well microtiter dish from 16 hours for the current DHPLC system to less than two hours. Another limitation of the current DHPLC system is the need to establish specific temperatures for distinct types of mutations for optimal mutation detection sensitivity. The SCE9610 system circumvents this limitation by its capacity to perform sample analysis over a precisely controlled temperature gradient within a single run, eliminating the need for temperature optimization to maximize the detection sensitivity. The combination of massive multiplexing, high separation efficiency, fast analysis time, full automation and sensitive detection makes the SCE9610 a highly suitable analytical platform for high-throughput applications for many projects on the UNC-Chapel Hill campus.