A CAROLINA CENTER TO CHARACTERIZE & MAINTAIN MUTANT MICE: AIDS

卡罗来纳州的特色中心

• 批准号: 7360410
• 负责人: TERRY MAGNUSON
• 金额: $ 29.28万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7360410
• 关键词: CAROLINA; CENTER; CHARACTERIZE; MAINTAIN; MUTANT

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Regarding AIDS DESCRIPTION (provided by applicant): Due to the recent completion of the human and mouse genomes as well as advances in mouse technologies, there has been an enormous increase in the number of mouse mutants and models for human disease. Maintaining and distributing pathogen-free mouse strains is both labor-intensive and costly for individual investigators to sustain longterm. The NIH/NCRR recognized the need for a common repository and distribution center for genetically modified mice, and in 1999 they responded by funding the Mutant Mouse Regional Resource Centers (MMRRC). UNC-Chapel Hill is one of four MMRRC centers that are developing this resource for the benefit of the entire biomedical community. Since the initial funding in 1999, a significant amount of effort has been invested in creating the organizational infrastructure and establishing standard operating procedures (SOPs) for importation, rederivation, phenotyping, cryopreservation and distribution of mutant mouse strains. In 2001, the MMRRC-UNC accepted its first strain and has experienced significant-expansion ever since. In parallel with these efforts, the MMRRC has also taken advantage of the expertise available at UNC to bring new technologies and resources to the community such as mutant ES-cell lines, gnotobiotic strains and improved in vitro fertilization methods. To meet future demands for MMRRC services and improve upon existing technologies, this proposal aims to: (1) import, archive and distribute genetically modified mouse strains, (2) increase visibility of the MMRRC through advertising and solicitations targeting the biomedical research community, (3) develop improved and reliable methods for assisted fertilization, (4) integrate gnotobiotic strain development into MMRRC services (5) generate an allelic series of point mutations in genes of interest as a community resource.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。关于艾滋病描述（由申请人提供）：由于最近人类和小鼠基因组的完成以及小鼠技术的进步，小鼠突变体和人类疾病模型的数量大幅增加。维持和分发无病原体的小鼠品系是一项劳动密集型的工作，对于个体研究者来说，长期维持是一项昂贵的工作。NIH/NCRR认识到有必要为转基因小鼠建立一个共同的储存库和分配中心，并在1999年资助了突变小鼠区域资源中心（MMRRC）。北卡罗来纳大学教堂山分校是为整个生物医学界开发这一资源的四个MMRRC中心之一。自1999年获得初始资金以来，在创建组织基础设施和建立标准操作程序（sop）方面投入了大量精力，用于突变小鼠品系的输入、再衍生、表型分型、冷冻保存和分发。2001年，MMRRC-UNC接受了它的第一个菌株，并从那时起经历了显著的扩展。与此同时，MMRRC还利用了UNC的专业知识，为社区带来了新的技术和资源，如突变es细胞系、非生物菌株和改进的体外受精方法。为了满足未来对MMRRC服务的需求并改进现有技术，本提案旨在：(1)导入、存档和分发转基因小鼠品系；(2)通过针对生物医学研究界的广告和募捐活动提高MMRRC的知名度；(3)开发改进的、可靠的辅助受精方法；(4)将非生物品系开发纳入MMRRC服务；(5)在感兴趣的基因中产生一系列等位基因点突变，作为社区资源。