UTMB Amino Acid Analyzer

描述(由申请人提供)： 需要支持日立L-8800型氨基酸分析仪，为德克萨斯大学加尔维斯顿分校(UTMB)相对较大的生物医学研究人员进行广泛的氨基酸分析。日立分析仪采用柱后方法，利用比色检测(金刚三酮)或荧光检测(奥米克-邻苯二甲酸二乙酯)。此外，L-8800可以执行各种分析方法，包括蛋白质/多肽水解物、生理液体和特殊样品的水解物，如纤维蛋白质或经过化学或翻译后修饰的蛋白质的水解物。蛋白质/肽浓度的组成分析和精确分析将是日立分析仪的关键应用之一，以及生理液体和组织提取液样本中的游离氨基酸分析。所要求的日立分析仪将取代已退役15年的Beckman 6300和已老化13年的应用生物系统420H PTC-氨基酸分析仪。日立分析仪将被UTMB的许多NIH资助的研究调查人员使用；描述了一个具有代表性的用户群体，拥有14笔NIH资助的赠款，其中包括NIH资助的五个主要和五个小用户，其中包括来自UTMB几个部门的多学科调查人员，例如人类生物化学和遗传学、微生物学和毒理学、预防医学和社区卫生以及海洋生物医学研究所。这些用户正在研究的生物医学问题或疾病的例子包括胃肠道细菌致病机制、外源生物、糖尿病和老年性白内障的发生、环境毒理学和诱变。氨基酸分析仪将在UTMB生物分子资源设施内的蛋白质化学核心内操作和维护，UTMB生物分子资源设施是一个长期的研究支持设施，包括五个核心，即蛋白质化学、多肽合成、蛋白质表达和纯化、质谱学和蛋白质组学。这些核心一起为整个大学校园提供广泛的研究服务，包括多个校园研究中心。购买所需的仪器将显著提高NIH资助的研究人员进行持续的假设驱动研究的能力，而且还将把他们未来的研究机会扩展到更高水平的生产力和科学影响。

DESCRIPTION (provided by applicant): Support is requested for a Hitachi Model L-8800 amino acid analyzer to perform a wide spectrum of amino acid analyses for a relatively large group of biomedical researchers at the University of Texas Medical Branch at Galveston (UTMB). The Hitachi analyzer employs a post-column methodology utilizing either colorimetric detection (ninhydrin) or fluorescence detection (omicron-phthaladehyde). Furthermore, the L-8800 can perform a wide variety of analytical methodologies including protein/peptide hydrolyzates, physiological fluids, and hydrolyzates of specialized samples, such as those of fibrous proteins, or proteins that have been chemically or posttranslationally modified. Compositional analysis and precise analysis of protein/peptide concentrations will be among the critically important applications of the Hitachi analyzer, as well as analysis of free amino acids in samples of physiological fluids and tissue extracts. The requested Hitachi analyzer will replace a 15-year old retired Beckman 6300 and an aging 13-year old Applied Biosystems 420H Ptc-amino acid analyzer. The Hitachi analyzer will be used by many NIH-funded research investigators at UTMB; a representative user group, with 14 funded NIH grants, is described that includes five major and five minor NIH-funded users, who include multidisciplinary investigators from several departments at UTMB, e.g. Human Biological Chemistry and Genetics, Microbiology and Toxicology, Preventive Medicine and Community Health, and the Marine Biomedical Institute. Examples of biomedical questions or diseases under investigation by these users include gastrointestinal bacterial pathogenesis, xenobiotics, diabetic and senile cataractogenesis, environmental toxicology and mutagenesis. The amino acid analyzer will be operated and maintained in the Protein Chemistry Core within the UTMB Biomolecular Resource Facility, a longstanding research support facility that includes five cores, namely, Protein Chemistry, Peptide Synthesis, Protein Expression and Purification, Mass Spectrometry, and Proteomics. Together, these Cores provide a wide range of research services for the entire University campus, including multiple campus research centers. Acquisition of the requested instrumentation will significantly enhance the ability of NIH-funded investigators to conduct their ongoing hypothesis-driven research and, moreover, will extend their research opportunities in the future to higher levels of productivity and scientific impact.