Multiple Peptide Synthesizer - An Integrated System

描述(由申请人提供)：请求支持自动 固相多肽合成器、制备型高效液体 专门用于多肽纯化的层析设备，以及真空 用于干燥合成和提纯过程中使用的溶剂的浓缩器。这 生产合成肽的集成仪器系统将被取代 已有十年历史的老旧设备，现在没有制造商的支持。这个 选择合成器，级联的雨宁交响乐/多路复用器 组合反应器，是一种多肽合成器，可以合成 到十二个肽同时独立，让我们更好地相遇 美国国立卫生研究院资助的研究人员对合成肽的需求增加 德克萨斯大学医学院分校(UTMB)。的受益用户 所要求的设备包括一个由14个多学科组成的小组，主要是 NIH支持的调查人员(9个主要用户和5个次要用户；22个由NIB资助的赠款 每年总计400万美元的直接成本)，相当于几个临床 和UTMB的基础科学系，例如人类生物化学和 遗传学、微生物学和免疫学、儿科学、病理学、解剖学和 神经科学，以及内科。下列疾病的例子 这些合成肽使用者的调查包括涉及或 与肌肉营养不良，过敏，哮喘，癌症，外源药物， 环境毒理学、诱变和胃肠病学。多肽 合成核心是UTMB蛋白质化学的五个核心之一 实验室；其他是蛋白质/DNA测序，蛋白质组学，质子学 光谱和蛋白质表达核心。这些核心设施加在一起， 为整个大学校园提供广泛的服务，包括 多个校园研究中心。获取所请求的仪器 将显著增强NIH资助的调查人员进行 他们正在进行的以假设为导向的研究，而且还将扩展他们的 未来的研究机会提升到更高水平的生产力和 科学影响。

DESCRIPTION (provided by applicant): Support is requested for an automated solid-phase peptide synthesizer, preparative high-performance liquid chromatography equipment dedicated to peptide purification, and a vacuum concentrator for drying solvents used during synthesize and purification. This integrated system of instruments for producing synthetic peptides will replace aging ten-year-old equipment that is now unsupported by the manufacturer. The selected synthesizer, a Rainin Symphony/Multiplex with the Cascade Combinatorial Reactor, is a multiple peptide synthesizer that can synthesize up to twelve peptides simultaneously and independently, allowing us to better meet increased demands for synthetic peptides for NIH-funded researchers at the University of Texas Medical Branch (UTMB). The beneficiary users of the requested equipment include a multidisciplinary group of fourteen predominantly NIH-supported investigators (9 major and 5 minor users; 22 funded NIB grants totaling $4 million dollars annual direct costs) representing several clinical and basic science departments at UTMB, e.g. Human Biological Chemistry and Genetics, Microbiology and Immunology, Pediatrics, Pathology, Anatomy and Neuroscience, as well as Internal Medicine. Examples of diseases under investigation by these synthetic peptide users include those involving or related to muscular dystrophy, allergy, asthma, cancer, xenobiotics, environmental toxicology, mutagenesis, and gastroenterology. The Peptide Synthesis Core is one of five cores within the UTMB Protein Chemistry Laboratory; the others are the Protein/DNA Sequencing, the Proteomics, the Mass Spectrometry, and the Protein Expression Cores. Together, these core facilities provide a wide range of services for the entire University campus including multiple campus research centers. Acquisition of the requested instrumentation will significantly enhance the ability of NIH-funded investigators to conduct their ongoing hypothesis-driven research and, moreover, will extend their research opportunities in the future to higher levels of productivity and scientific impact.