Multiple Peptide Synthesizer - An Integrated System

描述（由申请人提供）：要求支持自动化 固相多肽合成仪，制备型高效液相 专用于肽纯化的色谱设备，以及真空 用于干燥合成和纯化过程中使用的溶剂的浓缩器。这 用于生产合成肽的集成仪器系统将取代 使用了10年的旧设备，现在制造商不再支持它了。的 精选合成器，Rainin Symphony/Multiplex with the Cascade 组合反应器，是一个多肽合成器，可以合成 12种肽同时和独立，使我们能够更好地满足 美国国立卫生研究院资助的研究人员对合成肽的需求增加 德克萨斯大学医学分支（UTMB）。的受益用户 所要求的装备包括一个由14人组成的多学科小组， NIH支持的研究者（9个主要用户和5个次要用户; 22个资助的NIB赠款 总计400万美元的年度直接成本），代表了几个临床 和基础科学部门在UTMB，例如人类生物化学和 遗传学、微生物学和免疫学、儿科学、病理学、解剖学和 神经科学，以及内科。以下疾病的例子 这些合成肽使用者的研究包括那些涉及或 与肌肉萎缩症，过敏，哮喘，癌症，异生物质， 环境毒理学、诱变和胃肠病学。所述肽 合成核心是UTMB蛋白质化学的五个核心之一 实验室;其他是蛋白质/DNA测序，蛋白质组学，质量 光谱分析和蛋白质表达核心。这些核心设施 为整个大学校园提供广泛的服务，包括 多个校园研究中心。购置所要求的仪器 将大大提高NIH资助的研究人员进行 他们正在进行的假设驱动的研究，而且，将扩大他们的 未来的研究机会，以更高的生产力水平， 科学影响。

DESCRIPTION (provided by applicant): Support is requested for an automated solid-phase peptide synthesizer, preparative high-performance liquid chromatography equipment dedicated to peptide purification, and a vacuum concentrator for drying solvents used during synthesize and purification. This integrated system of instruments for producing synthetic peptides will replace aging ten-year-old equipment that is now unsupported by the manufacturer. The selected synthesizer, a Rainin Symphony/Multiplex with the Cascade Combinatorial Reactor, is a multiple peptide synthesizer that can synthesize up to twelve peptides simultaneously and independently, allowing us to better meet increased demands for synthetic peptides for NIH-funded researchers at the University of Texas Medical Branch (UTMB). The beneficiary users of the requested equipment include a multidisciplinary group of fourteen predominantly NIH-supported investigators (9 major and 5 minor users; 22 funded NIB grants totaling $4 million dollars annual direct costs) representing several clinical and basic science departments at UTMB, e.g. Human Biological Chemistry and Genetics, Microbiology and Immunology, Pediatrics, Pathology, Anatomy and Neuroscience, as well as Internal Medicine. Examples of diseases under investigation by these synthetic peptide users include those involving or related to muscular dystrophy, allergy, asthma, cancer, xenobiotics, environmental toxicology, mutagenesis, and gastroenterology. The Peptide Synthesis Core is one of five cores within the UTMB Protein Chemistry Laboratory; the others are the Protein/DNA Sequencing, the Proteomics, the Mass Spectrometry, and the Protein Expression Cores. Together, these core facilities provide a wide range of services for the entire University campus including multiple campus research centers. Acquisition of the requested instrumentation will significantly enhance the ability of NIH-funded investigators to conduct their ongoing hypothesis-driven research and, moreover, will extend their research opportunities in the future to higher levels of productivity and scientific impact.