Murine Cardiovascular MRI

描述(由申请人提供)： 爱荷华大学的研究人员请求支持购买瓦里安Unitylnova 4.7T磁共振成像系统，用于活体小鼠研究。这些研究人员由来自爱荷华大学3所学院8个系的研究人员组成，他们的级别从助理教授到系主任不等。他们都有着持久的热情，追求对心血管疾病的机械性理解。从综合生理学到分子生物学，再到过渡金属络合物的物理化学，这些研究的背景和学科应运而生。 所要求的仪器惊人的空间和时间分辨率将用于提供特定生理状态和疾病过程中器官功能的非侵入性定量评估。将对有梗死后心力衰竭、遗传性扩张型心肌病倾向、左心室压力超负荷、氯通道缺陷、败血症和缺血再灌注损伤的小鼠进行局部心肌收缩功能的评估。我们将在患有动脉粥样硬化、糖尿病和压力超负荷的小鼠身上进行成像。患有糖尿病和动脉粥样硬化的小鼠的脑梗塞和微血管功能将被成像。将在遗传和饮食诱导的肥胖小鼠模型中研究体内脂肪分布及其对心血管功能的影响。肿瘤血管生成拟态的革命性概念将具有生理学特征。将开发和测试用于心血管结构和功能成像以及用于基因转移体内成像的新型造影剂。 在这些技术和生物医学领域存在着大量的地方专门知识。这些项目旨在充分利用所请求的仪器目前已知的能力，而不需要未来的硬件或软件开发。爱荷华大学已经投入了大量的财政和行政资源，使用所要求的工具成功地和富有成效地追求生物医学知识。

DESCRIPTION (provided by applicant): Investigators from the University of Iowa request support to purchase a Varian Unitylnova 4.7T Magnetic Resonance Imaging System for study of mice in vivo. These investigators comprise a convivial and collegial group of research faculty from 8 departments in 3 colleges within the University of Iowa, ranging in rank from Assistant Professor to Department Head. They share an abiding passion for pursuit of mechanistic understanding of cardiovascular disease. These pursuits arise from backgrounds and disciplines ranging from integrative physiology to molecular biology to the physical chemistry of transition metal complexes. The spectacular spatial and temporal resolution of the requested Instrument will be used to provide non-invasive quantitative assessment of organ function in specific physiological states and disease processes. Regional myocardial systolic function will be assessed in mice with post-infarction heart failure, inherited proneness to dilated cardiomyopathy, left ventricular pressure-overload, chloride channel deficiency, sepsis, and ischemia-reperfusion injury. Remodeling of the great vessels and will be imaged in mice with atherosclerosis, diabetes, and pressure-overload. Cerebral infarction and microvascular function will be imaged in mice with diabetes and atherosclerosis. Corporeal adipose distribution and its consequent effects on cardiovascular function will be studied in genetic and diet-induced mouse models of obesity. The revolutionary concept of tumor vasculogenic mimicry will be physiologically characterized. New contrast agents for imaging cardiovascular structure and function, and for in vivo imaging of gene transfer, will be developed and tested. Significant local expertise in these technical and biomedical fields exists. The Projects are designed to fully utilize the present known capabilities of the requested Instrument, without requiring future hardware or software development. The University of Iowa has committed substantial financial and administrative resources toward successful and productive pursuit of biomedical knowledge using the requested Instrument.