LMS510 Confocal Microscope/Cell Imaging Shared Resource

描述（申请人提供）：该申请是为了资金购买蔡司LSM 510共聚焦显微镜，以取代大量使用9年的LSM 410，范德比尔特大学细胞成像共享资源（CISR）的主要主力目前，CISR为150多个VUMC实验室提供服务，72个实验室使用LSM 410来支持他们的研究，大多数由PHS资助，CISR具有优良的服务、可靠性和最短的停机时间的历史。除了对不间断服务的重要要求之外，对使用先进技术的现代活体标本成像的需求也在不断增长，例如FRAP、FLIP、FRET，快速顺序行扫描（多跟踪），以及我们的LSM 410不支持的其他功能最近收购的LSM 510系统，以支持五个主要用户组的高级应用，这使得新用户、总共焦成像时间以及新研究者和新应用的可行性研究增加。LSM 410的使用已经增加，LSM 510上的非主要用户组可用的时间正在从研究人员那里迅速吸收，这些研究人员由于LSM 410缺乏新应用的功能而无法使用LSM 410。2年内无需更换LSM 410新系统这些预期是基于需求的预计增长和关键更换部件供应的减少，以维持不间断的服务重建或抢救的组件支持这些期望该应用程序是一个积极的步骤，以防止由于大量使用的仪器不可避免的衰变而导致的延迟和丢失NIH资助的研究的灾难，并提高共聚焦显微镜数据的质量和数量。

DESCRIPTION (provided by applicant): This application is for funds to buy a Zeiss LSM510 confocal microscope to replace a heavily used 9-year old LSM410, the main workhorse of the Vanderbilt University Cell Imaging Shared Resource (CISR) Currently, the CISR provides service to more than 150 VUMC laboratories, 72 laboratories use the LSM410 to support their research, most PHS-funded, to the extent of >2300 hours per year, trained users can access the facility 24 hours/day, 7 days a week The CISR has a history of excellent service, reliability and minimal down time In addition to the vital requirement for uninterrupted service, there is an increasing demand for modern live specimen imaging using advanced techniques, e g, FRAP, FLIP, FRET, rapid sequential line scanning (multi-tracking), and other capabilities not supported by our LSM410 The recent acquisition of an LSM510 system to support advanced applications of five major user groups has enabled increases in new users, total confocal imaging time, and feasibility studies for new investigators and new applications In spite of the additional system, the use of the LSM410 has increased and the time available to non-major user groups on the LSM510 is being rapidly absorbed from researchers unable to use the LSM410 due to its lack of functionality for new applications Significant disruptions and delays in confocal microscope-dependent research are anticipated within the next 1-2 years in lieu of replacement of the LSM410 with a new system These expectations are based on a projected increase in demand and a dwindling supply of key replacement components to sustain uninterrupted service Recent experience with obtaining critical spare parts available only from surplus, rebuilt or salvaged components support these expectations This application is a proactive step to prevent a disaster in delayed and lost NIH-funded research due to the inevitable decay of a heavily used instrument and to increase the quality and quantity of confocal microscope data.