LMS510 Confocal Microscope/Cell Imaging Shared Resource

描述(由申请人提供)：此申请是为购买蔡司LSM510共焦显微镜资金，以取代频繁使用的9岁的LSM410，范德比尔特大学细胞成像共享资源(CISR)的主要主力。目前，CISR为150多个VUMC实验室提供服务，72个实验室使用LSM410支持他们的研究，大多数是PHS资助的，在&GT的范围内；每年2300个小时，训练有素的用户可以一周7天、每天24小时访问该设施除了对不间断服务的重要要求外，CISR还拥有卓越的服务、可靠性和最短的停机时间，对使用先进技术的现代活体标本成像的需求日益增长，例如FRAP、Flip、FRET、快速顺序线扫描(多跟踪)和其他我们的LSM410系统不支持的其他功能。最近购买了LSM510系统以支持五个主要用户组的高级应用程序，从而增加了新用户、总共焦成像时间，以及新调查人员和新应用的可行性研究，尽管增加了系统，LSM410的使用已经增加，并且LSM510上的非主要用户组可用的时间正在从由于LSM410缺乏用于新应用的功能而不能使用LSM410的研究人员那里被迅速吸收。预计在未来1-2年内，依赖于共焦显微镜的研究将出现显著的中断和延迟，而不是用新系统替换LSM410。这些预期基于需求的预计增加和关键替换部件的供应减少，以维持不间断的服务。最近获得只能从过剩中获得的关键备件的经验，重建或回收的部件支持这些预期，该应用程序是一个积极的步骤，可以防止NIH资助的研究因大量使用的仪器不可避免地腐烂而发生灾难，并提高共聚焦显微镜数据的质量和数量。

DESCRIPTION (provided by applicant): This application is for funds to buy a Zeiss LSM510 confocal microscope to replace a heavily used 9-year old LSM410, the main workhorse of the Vanderbilt University Cell Imaging Shared Resource (CISR) Currently, the CISR provides service to more than 150 VUMC laboratories, 72 laboratories use the LSM410 to support their research, most PHS-funded, to the extent of >2300 hours per year, trained users can access the facility 24 hours/day, 7 days a week The CISR has a history of excellent service, reliability and minimal down time In addition to the vital requirement for uninterrupted service, there is an increasing demand for modern live specimen imaging using advanced techniques, e g, FRAP, FLIP, FRET, rapid sequential line scanning (multi-tracking), and other capabilities not supported by our LSM410 The recent acquisition of an LSM510 system to support advanced applications of five major user groups has enabled increases in new users, total confocal imaging time, and feasibility studies for new investigators and new applications In spite of the additional system, the use of the LSM410 has increased and the time available to non-major user groups on the LSM510 is being rapidly absorbed from researchers unable to use the LSM410 due to its lack of functionality for new applications Significant disruptions and delays in confocal microscope-dependent research are anticipated within the next 1-2 years in lieu of replacement of the LSM410 with a new system These expectations are based on a projected increase in demand and a dwindling supply of key replacement components to sustain uninterrupted service Recent experience with obtaining critical spare parts available only from surplus, rebuilt or salvaged components support these expectations This application is a proactive step to prevent a disaster in delayed and lost NIH-funded research due to the inevitable decay of a heavily used instrument and to increase the quality and quantity of confocal microscope data.