LMS510 Confocal Microscope/Cell Imaging Shared Resource

描述（由申请人提供）：这个应用程序是为资金买蔡司LSM510共焦显微镜来取代大量使用9岁LSM410,范德比尔特大学细胞成像的主要主力共享资源(信息系统)目前,信息提供服务超过150 VUMC实验室,72实验室使用LSM410来支持他们的研究,大多数PHS-funded,每年> 2300小时,训练有素的用户可以访问设备24小时/天,每周7天的信息有一个优质的服务的历史,除了对不间断服务的重要要求外，对使用先进技术的现代活体标本成像的需求不断增加，例如FRAP， FLIP， FRET，快速顺序线扫描（多跟踪）以及LSM410不支持的其他功能。最近收购的LSM510系统支持五大主要用户群体的先进应用，从而增加了新用户，总共聚焦成像时间，以及为新的研究人员和新的应用进行可行性研究。LSM410的使用增加了和时间可用在LSM510 non-major用户组被迅速吸收人员无法使用LSM410由于其缺乏对新应用程序的功能重要的中断和延迟共焦microscope-dependent研究预计在未来1 - 2年内代替取代LSM410新系统这些期望是基于预期的需求增加和减少供应键替换最近从剩余、重建或回收的组件中获得关键备件的经验支持了这些期望。该应用程序是一个积极的步骤，以防止由于大量使用的仪器不可避免的衰减而导致的灾难，并减少了nih资助的研究，并提高了共聚焦显微镜数据的质量和数量。

DESCRIPTION (provided by applicant): This application is for funds to buy a Zeiss LSM510 confocal microscope to replace a heavily used 9-year old LSM410, the main workhorse of the Vanderbilt University Cell Imaging Shared Resource (CISR) Currently, the CISR provides service to more than 150 VUMC laboratories, 72 laboratories use the LSM410 to support their research, most PHS-funded, to the extent of >2300 hours per year, trained users can access the facility 24 hours/day, 7 days a week The CISR has a history of excellent service, reliability and minimal down time In addition to the vital requirement for uninterrupted service, there is an increasing demand for modern live specimen imaging using advanced techniques, e g, FRAP, FLIP, FRET, rapid sequential line scanning (multi-tracking), and other capabilities not supported by our LSM410 The recent acquisition of an LSM510 system to support advanced applications of five major user groups has enabled increases in new users, total confocal imaging time, and feasibility studies for new investigators and new applications In spite of the additional system, the use of the LSM410 has increased and the time available to non-major user groups on the LSM510 is being rapidly absorbed from researchers unable to use the LSM410 due to its lack of functionality for new applications Significant disruptions and delays in confocal microscope-dependent research are anticipated within the next 1-2 years in lieu of replacement of the LSM410 with a new system These expectations are based on a projected increase in demand and a dwindling supply of key replacement components to sustain uninterrupted service Recent experience with obtaining critical spare parts available only from surplus, rebuilt or salvaged components support these expectations This application is a proactive step to prevent a disaster in delayed and lost NIH-funded research due to the inevitable decay of a heavily used instrument and to increase the quality and quantity of confocal microscope data.