Imaging Core

成像核心的目的是为以下项目的参与者提供设施、资源、培训和支持 PPG用于明场和荧光成像作为显示和定位活性氧的实验工具 人和动物心血管和肾组织中的种类(ROS)和炎症成分 疾病的模型。成像核心的设施提供组织处理和组织学 准备、免疫组织化学和免疫细胞化学成像和激光扫描共聚焦 显微镜(LSCM)成像。成像核心工作人员将提供监督、维护、培训和支持 用于显微镜、组织处理设备、计算机和图像分析软件。最后， 经验丰富、技术熟练的影像专家将为规划和管理人员提供咨询服务 评估实验。因此，增加成像核心设施将显著增强 项目负责人可视化组织内ROS产生或炎性细胞入侵的位置 各种实验或病理条件。重要的是，这些形态数据将与 其他更定量的方法，如电子自旋共振(ESR)光谱测量ROS(in 核心A)和免疫印迹来测量细胞或组织中蛋白质的表达。

The purpose of the Imaging Core is to provide facilities, resources, training and support to participants of the PPG for brightfield and fluorescence imaging as experimental tools to visualize and localize reactive oxygen species (ROS) and inflammatory components in cardiovascular and renal tissues in humans and animal models of disease. The facilities of the Imaging Core provide for tissue processing and histological preparations, immunohistochemical and immunocytochemical imaging, and laser scanning confocal microscopy (LSCM) imaging. The Imaging Core staff will offer supervision, maintenance, training and support for the microscopes, tissue processing equipment, computers and image analysis software. Finally, experienced and skilled imaging experts on the staff will provide consultative services for planning and evaluating experiments. Thus the addition of the Imaging Core facility will significantly enhance the ability of project leaders to visualize the locations of ROS production or inflammatory cell invasion within tissues under a variety of experimental or pathological conditions. Importantly, these morphological data will be correlated with other more quantitative methods, such as electron spin resonance (ESR) spectroscopy to measure ROS (in Core A) and immunoblotting to measure expression of proteins within cells or tissues.