Cell Imaging Shared Resource

细胞成像共享资源

• 批准号: 9330511
• 负责人: K. Sam Wells
• 金额: $ 9.09万
• 依托单位: VANDERBILT UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9330511
• 关键词: Capital; Caring; Cell physiology; Confocal Microscopy; Consult; Consultations; Data Analyses; Development; Diabetes Mellitus; Electron Microscopy; Ensure; Equipment; Experimental Designs; Fee-for-Service Plans; Fluorescence; Funding; Goals; Grant; Image; Image Analysis; Imaging Techniques; Imaging technology; Individual; Laboratories; Laser Scanning Microscopy; Methodology; Microscopy; Modernization; Optics; Preparation; Process; Quality Control; Research; Research Personnel; Research Support; Research Training; Resolution; Resource Sharing; Resources; Scanning Electron Microscopy; Schedule; Services; Specimen; Subcellular Anatomy; Supporting Cell; System; Systems Analysis; Tissues; Training; Transmission Electron Microscopy; Update; career development; cellular imaging; collaborative environment; cost; digital imaging; experimental study; image processing; imaging capabilities; in vivo; in vivo imaging; innovation; instrument; intravital imaging; medical specialties; member; microscopic imaging; multidisciplinary; organizational structure; prevent; skills; technology development; two-photon; web based interface

Cell Imaging Shared Resource (CISR): Project Summary/Abstract The Vanderbilt Diabetes Research and Training Center (VDRTC) will continue to support the VDRTC Cell Imaging Core, operated by the Cell Imaging Shared Resource (CISR), and ensure that VDRTC-affiliated investigators will have full access to state-of-the-art imaging equipment and expert technical support to conduct sophisticated microscopy and analysis of tissue and cellular anatomy and physiology. By utilizing a large, well- established facility, the VDRTC Cell Imaging Core provides high levels of quality control, a dedicated, expert staff and a significant economy of scale for services that would be difficult (if not impossible) for individual investigators to establish and support. This organizational structure fits well with the centralized approach to prevent needless duplication of scientific resources within federally funded research centers, enabling focused development of technology that serves all Vanderbilt research, including VDRTC investigators. The overall goal of the VDRTC Cell Imaging Core is to provide the full range of modern microscopy and digital imaging capabilities and techniques to enable and accelerate diabetes research at Vanderbilt through three objectives: 1) acquire and maintain state-of-the art optical and EM imaging technology; 2) train, assist, and encourage VDRTC-affiliated investigators to incorporate optical, EM, and in vivo imaging technologies into their research; and 3) develop new imaging technologies that will be useful for diabetes research. Without VDRTC support, the breadth and quality of advanced microscopy service would be severely limited. The VDRTC support grant also reduces the net cost of imaging service to VDRTC investigators and has helped provide financial stability essential to sustain historically high levels of service to VDRTC researchers. This stability has been vital to the successful acquisition of new instruments and services that support VDRTC researchers. During the past 5 years, investigators representing 84 separate VDRTC-affiliated laboratories (a 31% increase from the prior cycle) have used all aspects of the CISR. Over the same period, CISR initiatives taken to obtain equipment grants, combined with institutional contributions, have provided $4,766,063 in new capital equipment, managed by the CISR, for the benefit of VDRTC investigators. This includes super-resolution, 2-photon-excited fluorescence, multi-excitation TIRF, and other advanced microscopies. The Vanderbilt shared facilities system provides an efficient billing system, oversight and governance for the Cell Imaging Core. The VDRTC Cell Imaging Core will continue to be a critical component in Vanderbilt's broad range of diabetes research resources and will provide essential services that support the research of VDRTC-affiliated investigators during the next funding cycle.

细胞成像共享资源（CISR）：项目摘要/摘要 范德比尔特糖尿病研究和培训中心（VDRTC）将继续支持VDRTC细胞 成像核心，由细胞成像共享资源（CISR）运营，并确保VDRTC附属 调查人员将有充分的机会使用最先进的成像设备和专家技术支持， 组织和细胞解剖学和生理学的精密显微镜和分析。利用一个大的，好的- VDRTC细胞成像核心是一个成熟的设施，提供高水平的质量控制，一个专门的专家， 工作人员和显著规模经济的服务，这将是困难的（如果不是不可能的）个人 调查人员建立和支持。这种组织结构非常适合集中管理的办法， 防止联邦资助的研究中心内不必要的科学资源重复， 为所有范德比尔特研究提供服务的技术开发，包括VDRTC研究人员。整体 VDRTC细胞成像核心的目标是提供全方位的现代显微镜和数字成像 能力和技术，使和加速糖尿病研究在范德比尔特通过三个目标： 1)获取和维护最先进的光学和EM成像技术; 2）培训，协助和鼓励 VDRTC附属研究人员将光学、EM和体内成像技术纳入其研究; 以及3）开发将对糖尿病研究有用的新的成像技术。如果没有VDRTC的支持， 先进显微镜服务的广度和质量将受到严重限制。VDRTC支持赠款 还降低了VDRTC调查人员的成像服务净成本，并有助于提供财务稳定性 对于维持对VDRTC研究人员的历史高水平服务至关重要。这种稳定性对 成功获得支持VDRTC研究人员的新仪器和服务。过去5 年，研究人员代表84个独立的VDRTC附属实验室（比上一年增加31%）， 循环）使用了CISR的所有方面。在同一时期，CISR为获得设备而采取的举措 赠款与机构捐款相结合，提供了4，766，063美元的新资本设备，管理 由CISR提供，以供VDRTC调查人员使用。这包括超分辨率，双光子激发 荧光，多激发TIRF，和其他先进的显微镜。范德比尔特共享设施系统 为Cell Imaging Core提供高效的计费系统、监督和管理。VDRTC单元 Imaging Core将继续成为范德比尔特广泛的糖尿病研究的关键组成部分 资源，并将提供必要的服务，支持VDRTC附属研究人员的研究， 下一个融资周期。