LSM880-Airyscan Confocal Microscope for Large Shared Resource

 描述（由申请人提供）：我们申请资金购买卡尔蔡司LSM880与Airyscan共聚焦显微镜的基础上Axio观察员Z1倒置机构。主要目标是取代使用了12年的蔡司LSM 510 Meta，该设备是由范德比尔特基金收购的，并于2003年集成到范德比尔特细胞成像共享资源（CISR）中。带有Airyscan的LSM 880在范德比尔特是独一无二的，对于支持代表范德比尔特大学11个部门的8个主要用户和10个次要用户组正在进行的NIH资助的研究至关重要。这些研究人员在广泛的研究中对细胞解剖学和生理学的高级成像有基本需求，包括神经病理学和青光眼，神经元存活，肥胖和糖尿病，囊泡运输和癌症，心律失常，膜生物物理学，上皮形态发生和癌症，以及肠神经系统中的神经嵴谱系。我们希望，许多其他NIH资助的项目将得到很好的支持，特别是那些需要活细胞和组织成像。我们选择LSM880和Airyscan作为最佳仪器是基于三个主要目标：1）提高活细胞和组织成像的能力，2）提高复杂多通道荧光显微镜和其他先进技术的高分辨率成像能力，3）保持大量正在进行的研究的连续性，这些研究对共聚焦显微镜有基本需求。所要求的仪器根据主要用户和次要用户的需求进行配置，重点考虑光学性能、衍射极限下的高速图像采集，同时保持活细胞生理条件。这些规格是成功实施主要用户项目中描述的先进共焦成像的基础。仪器将位于医学研究大楼III（4155 MRBIII室），并由细胞成像共享资源（CISR）维护。这个房间是一个CISR显微镜设施，由CISR专门维护，为所有范德比尔特研究人员的利益。这个空间是安全的，所有主要用户都可以轻松访问。这一文书的专门知识和机构支持是非同寻常的。CISR主任威尔斯博士是国家公认的显微镜专家，在共聚焦、多光子激发荧光和其他先进成像方法方面拥有丰富的经验。CISR拥有21年的历史，为300多个研究实验室提供尖端的成像专业知识和合作研究支持。VUMC承诺提供10万美元，以支持该仪器的长期运行。

 DESCRIPTION (provided by applicant): We request funds to purchase a Carl Zeiss LSM880 with Airyscan confocal microscope based on an Axio Observer Z1 inverted body. The main aim is to replace a heavily used 12-year old Zeiss LSM510 Meta, acquired with Vanderbilt funds and integrated into the Vanderbilt Cell Imaging Shared Resource (CISR) in 2003. The LSM880 with Airyscan will be unique at Vanderbilt and crucial to support the ongoing NIH-funded research of 8 Major User and 10 Minor User groups representing 11 departments within Vanderbilt University. These investigators have essential needs for advanced imaging of cellular anatomy and physiology in a wide range of investigations including, neuropathology and glaucoma, neuronal survival, obesity and diabetes, vesicle trafficking and cancer, cardiac arrhythmia, membrane biophysics, epithelial morphogenesis and cancer, and neural crest lineages in the enteric nervous system. We expect that numerous other NIH-funded projects will be well supported by this instrument; particularly those that require live cell and tissue imaging. We chose the LSM880 with Airyscan as the optimal instrument based on three main goals: 1) to improve the capabilities for live cell and tissue imaging, 2) to improve the capabilities for high-resolution imaging of complex, multi-channel fluorescence microscopy and other advanced techniques, and 3) to maintain continuity with a large number of ongoing investigations with essential needs for confocal microscopy. The requested instrument is configured based on the needs of the Major and Minor Users with significant consideration given to optical performance, high-speed image acquisition at the diffraction-limit, while maintaining live cell physiological conditions. The specifications are fundamental to the successful implementation of the advanced confocal imaging described in the Major User projects. The instrument will be located in Medical Research Building III (room 4155 MRBIII), and maintained by the Cell Imaging Shared Resource (CISR). This room is a CISR microscopy facility, maintained exclusively by the CISR, for the benefit of all Vanderbilt investigators. This space is secure and easily accessible to all major users. The expertise and institutional support for this instrument are exceptional. Dr. Wells, Director of the CISR, is a nationally recognized microscopy expert with experience in confocal, multi-photon-excited fluorescence, and other advanced imaging methods. The CISR has a 21-year history of assisting over 300 investigator labs with cutting-edge imaging expertise and cooperative research support. The VUMC has committed $100,000 to support the long-term operation of the instrument.