Imaging Core

成像核心

• 批准号: 8438265
• 负责人: K. Sam Wells
• 金额: $ 19万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2015-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8438265
• 关键词: Animal Disease Models; Blood Vessels; Cardiovascular Diseases; Cardiovascular system; Cells; Computer Assisted; Computer software; Core Facility; Data; Electron Spin Resonance Spectroscopy; Equipment; Fluorescence; Histology; Human; Image; Image Analysis; Imaging technology; Immunoblotting; Immunohistochemistry; Individual; Inflammation; Inflammatory; Laser Scanning Confocal Microscopy; Light Microscope; Location; Maintenance; Measures; Methods; Microscope; Paraffin; Participant; Preparation; Production; Reactive Oxygen Species; Renal Tissue; Resources; Services; Supervision; System; Tissues; Training Support; cryostat; digital imaging; experience; fluorescence imaging; immunocytochemistry; oxidant stress; protein expression; research study; tissue processing; tool

The purpose of the Imaging Core is to provide facilities, resources, training and support to participants of the PPG for brightfield and fluorescence imaging as experimental tools to visualize and localize reactive oxygen species (ROS) and inflammatory components in cardiovascular and renal tissues in humans and animal models of disease. The facilities of the Imaging Core provide for tissue processing and histological preparations, immunohistochemical and immunocytochemical imaging, and laser scanning confocal microscopy (LSCM) imaging. The Imaging Core staff will offer supervision, maintenance, training and support for the microscopes, tissue processing equipment, computers and image analysis software. Finally, experienced and skilled imaging experts on the staff will provide consultative services for planning and evaluating experiments. Thus the addition of the Imaging Core facility will significantly enhance the ability of project leaders to visualize the locations of ROS production or inflammatory cell invasion within tissues under a variety of experimental or pathological conditions. Importantly, these morphological data will be correlated with other more quantitative methods, such as electron spin resonance (ESR) spectroscopy to measure ROS (in Core A) and immunoblotting to measure expression of proteins within cells or tissues.

影像核心的目的是为参与者提供设施、资源、培训和支持， PPG用于明场和荧光成像，作为可视化和定位活性氧的实验工具 在人类和动物的心血管和肾组织中， 疾病的模型。成像中心的设施提供组织处理和组织学 制备、免疫组织化学和免疫细胞化学成像以及激光扫描共聚焦显微镜 显微镜（LSCM）成像。成像核心人员将提供监督、维护、培训和支持 用于显微镜、组织处理设备、计算机和图像分析软件。最后， 工作人员中经验丰富、技术熟练的成像专家将为规划和 评估实验。因此，增加成像核心设施将大大提高 项目负责人，以可视化ROS生产或炎症细胞入侵下的组织内的位置， 各种实验或病理条件。重要的是，这些形态学数据将与 其他更定量的方法，如电子自旋共振（ESR）光谱测量ROS（在 核心A）和免疫印迹以测量细胞或组织内的蛋白质表达。