ZEISS AXIOVERT 200 AUTOMATED 4-D CELL OBSERVER SYSTEM

ZEISS AXIOVERT 200 自动化 4D 细胞观察系统

• 批准号: 6581497
• 负责人: Steven D Leach
• 金额: $ 12.55万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6581497
• 关键词: biomedical equipment purchase; charge coupled device camera; fluorescence microscopy; intravital microscopy; light microscopy

DESCRIPTION (provided by applicant): Recent advances in automated robotics, high-resolution digital imaging and image deconvolution software now allow real-time documentation of dynamic processes in living cells and tissues. To take advantage of this progress, we have assembled an accomplished group of senior, mid-career, and junior investigators who wish to apply this emerging technology to their NIH-funded research projects. Together, these six Johns Hopkins faculty members serve as principle investigators on ten different NIH grants including nine R01 grants and one T32 training grant, together accounting for 51 years of cumulative uninterrupted NIH funding. These research projects are funded by six Jdifferent NIH institutes, reflecting a a wide array of research areas, including epithelial and hematopoietic stem cell biology, development biology, developmental neurobiology, macrophage function, and tumor biology. In order to apply recent advances in real time imaging to these projects, the investigators seek to purchase a fully integrated state-of-the-art imaging system comprised of a Zeiss Axiovert-200M motorized inverted fluorescent microscope, a Zeiss AxioCam high-resolution digital camera, a temperature / humidity / CO2-controlled stage chamber for maintenance of viable cell and tissue explants, and sophisticated Zeiss Axioversion software with 3D image deconvolution capability. Together, this Cell Observer System allows dynamic cellular processes to be observed at high resolution using multiple fluorescent channels over extended periods of time, generating new perspectives regarding integrated biologic processes in living tissues. This proposal outlines the scientific rational for use of this sophisticated imaging system, and further describes available institutional support as well as administrative and laboratory resources designed to promote optimal use of this shared instrumentation.

描述（由申请人提供）：自动化机器人技术，高分辨率数字成像和图像反卷积软件的最新进展现在允许实时记录活细胞和组织中的动态过程。为了利用这一进展，我们召集了一群有成就的高级、中级和初级研究人员，他们希望将这一新兴技术应用到他们的nih资助的研究项目中。这六名约翰霍普金斯大学的教师共同担任10项不同的NIH拨款的主要调查员，其中包括9项R01拨款和1项T32培训拨款，总共占51年累计不间断的NIH资助。这些研究项目由六个不同的NIH研究所资助，反映了广泛的研究领域，包括上皮和造血干细胞生物学，发育生物学，发育神经生物学，巨噬细胞功能和肿瘤生物学。为了将最新的实时成像技术应用到这些项目中，研究人员寻求购买一套完全集成的最先进的成像系统，包括一台蔡司Axiovert-200M电动倒置荧光显微镜、一台蔡司AxioCam高分辨率数码相机、一个用于维持活细胞和组织外体的温度/湿度/二氧化碳控制级室，以及具有3D图像反卷积能力的先进的蔡司Axioversion软件。总之，这个细胞观察系统允许在长时间内使用多个荧光通道以高分辨率观察动态细胞过程，产生关于活组织中综合生物过程的新视角。本提案概述了使用这种复杂成像系统的科学合理性，并进一步描述了为促进这种共享仪器的最佳使用而提供的机构支持以及行政和实验室资源。