DNA SEQUENCING SHARED RESOURCES

DNA测序共享资源为使用分子生物学方法、重组DNA技术、分子遗传学和人类遗传学的研究提供集中的、质量控制的和自动化的DNA测序。提供两种类型的DNA测序服务。第一，标准测序反应和电泳，研究者提供纯化的DNA片段（质粒、PCR产物、BAC）和测序引物（或要求使用资源维护的标准测序引物），测序实验室进行完整的测序反应和电泳。然后，通过电子邮件或文件传输向研究者提供包含个人测序结果的电子数据文件。他们还提供了所产生的每个反应的彩色电泳图。大量的DNA测序服务提供给调查者谁需要大规模测序有限

The DNA Sequencing Shared Resource offers centralized, quality-controlled and automated DNA sequencing for research using molecular biological methods, recombinant DNA techniques, molecular genetics, and human genetics. Two types of DNA sequencing services are provided. In the first, Standard Sequencing Reaction and Electrophoresis, investigators provide purified DNA temples (plasmid, PCR product, BAC) and sequencing primer (or request use of a standard sequencing primer maintained by the resource) and the sequencing laboratory performs a complete sequencing reaction and electrophoresis. The investigator is then provided electronic data files containing individual sequencing results distributed by e-mail or file transfer. They are also provided with a color electropherogram of each reaction generated. Bulk DNA Sequencing Services are available to investigators who need large-scale sequencing with a limited number of sequencing primers. Dr. Alfred L. George, Jr. is the Scientific Director of the DNA Sequencing Shared Resource. He oversees all activities of the Resource including quality control, user prioritization, personnel management, and new technology. He has a capable staff with many years of experience available to offer help to VICC investigators.