DNA SEQUENCING SHARED RESOURCES

DNA 测序共享资源

• 批准号: 6990154
• 负责人: Alfred L. George
• 金额: $ 3.98万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-28 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6990154
• 关键词: artificial chromosomes; biomedical facility; electrophoresis; genetic recombination; genetics; molecular biology; molecular genetics; neoplasm /cancer; nucleic acid sequence; plasmids; polymerase chain reaction

The DNA Sequencing Shared Resource offers centralized, quality-controlled and automated DNA sequencing for research using molecular biological methods, recombinant DNA techniques, molecular genetics, and human genetics. Two types of DNA sequencing services are provided. In the first, Standard Sequencing Reaction and Electrophoresis, investigators provide purified DNA temples (plasmid, PCR product, BAC) and sequencing primer (or request use of a standard sequencing primer maintained by the resource) and the sequencing laboratory performs a complete sequencing reaction and electrophoresis. The investigator is then provided electronic data files containing individual sequencing results distributed by e-mail or file transfer. They are also provided with a color electropherogram of each reaction generated. Bulk DNA Sequencing Services are available to investigators who need large-scale sequencing with a limited number of sequencing primers. Dr. Alfred L. George, Jr. is the Scientific Director of the DNA Sequencing Shared Resource. He oversees all activities of the Resource including quality control, user prioritization, personnel management, and new technology. He has a capable staff with many years of experience available to offer help to VICC investigators.

DNA测序共享资源为使用分子生物学方法，重组DNA技术，分子遗传学和人类遗传学的研究提供集中，质量控制和自动化的DNA测序。提供两种类型的DNA测序服务。在第一个标准测序反应和电泳中，研究人员提供纯化的DNA模板（质粒、PCR产物、BAC）和测序引物（或请求使用由资源维护的标准测序引物），测序实验室执行完整的测序反应和电泳。然后向研究者提供包含通过电子邮件或文件传输分发的个体测序结果的电子数据文件。还提供了所产生的每个反应的彩色电泳图。批量DNA测序服务可用于需要大规模测序的研究人员， 测序引物的数量。阿尔弗雷德·L.小乔治是DNA测序共享资源的科学总监。他监督资源的所有活动，包括质量控制，用户优先级，人员管理和新技术。他有一个有多年经验的能干的工作人员，可以为国际刑事法院调查人员提供帮助。