Bio contained sorter replacement

生物包含分选机更换

• 批准号: 8244762
• 负责人: ALBERT D DONNENBERG
• 金额: $ 59.97万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8244762
• 关键词: Award; Cell Separation; Cells; Chills; Consumption; Custom; Cytometry; Electronics; Engineering; Exercise; Failure; Fluorescence; Funding; Human; Lasers; Life; Pump; Recovery; Research Personnel; Resolution; Resources; Safety; Sampling; Services; Sorting - Cell Movement; Speed; Techniques; Testing; Time; Training; United States National Institutes of Health; Universal Precautions; University of Pittsburgh Cancer Institute; Update; Water; Writing; analog; digital; heat exchanger; instrument; instrumentation; photomultiplier

DESCRIPTION (provided by applicant): This proposal seeks funding to replace the flagship instrument in the University of Pittsburgh Cancer Institute (UPCI) Cytometry Facility (CF), our biocontained High Speed Sorter. The existing instrument, a MoFlo in which the fluidics components are contained within a certified Class I Safety Cabinet, was custom assembled 12 years ago through a Shared Instrumentation Award and $110,000 additional funding from the UPCI. Over its lifetime it has serviced literally hundreds of NIH-funded investigators, including many who make use of the ability to sort live human cells while exercising Universal Precautions. Because of our continuous efforts to update the MoFlo (adding lasers, PMT channels, 4-way and plate sorting, chilled sample station) this instrument has provided excellent service well beyond its expected service life. Despite CF Supervisor E. Michael Meyers' training as a MoFlo field service engineer, the MoFlo has been increasingly out of service (at the time of this writing it has been nonfunctional for 3 weeks due to failure of the obsolete laser water chiller/heat exchanger; the pump is being remanufactured). This places an unacceptable hardship on Facility Users, many of whom require our unique biocontainment capabilities, our expertise in multi-parameter multi-way sorting, and continuity in technique and instrumentation. The newly released Beckman-Coulter Astrios retains the functionality of our current instrument, replacing: 1) The class I biosafety cabinet with a well integrated class II cabinet; 2) Obsolete analog electronics with digital electronics; 3) Obsolete photomultipliers (PMTs) with higher sensitivity PMTs; 4) Energy inefficient water-cooled lasers with diode lasers, reducing energy consumption by 95%. The proposed Astrios will also provide a new laser line (561 nm) and increase the maximum number of fluorescence channels from 8 to 14. Through our own independent testing of the Astrios with beads and live cells, we have documented a significant increase in sensitivity, resolution, sort speed and recovery over our existing MoFlo. The requested enhancements will allow us to continue to provide the best possible cell sorting resource to our large group of NIH-funded investigators.

描述（由申请人提供）：该提案寻求资金来代替匹兹堡大学癌症研究所（UPCI）细胞仪设施（CF）的旗舰工具，这是我们的bioConted Hige Speed Sorter。现有的仪器是一种MOFLO，其中流体组件包含在经认证的I类安全机柜中，并通过共享仪器奖和UPCI额外的11万美元额外资金进行了定制组装。在其一生中，它已经为数百名NIH资助的研究人员提供服务，其中包括许多利用在行使普遍预防措施的同时对活细胞进行分类的能力。由于我们不断努力更新MOFLO（添加激光器，PMT频道，4路和板块分类，冷藏样品站），该乐器为超出了预期的使用寿命提供了出色的服务。尽管CF主管E. Michael Meyers担任MOFLO现场服务工程师的培训，但Moflo越来越多地服务（在撰写本文时，由于过时的激光水冷却器/热交换器的失败，它已经无功能了3周；泵已重新制造）。这给设施使用者带来了不可接受的艰辛，其中许多人需要我们独特的生物保化功能，我们在多参数多路分类方面的专业知识以及技术和仪器的连续性。新发布的Beckman-Coulter Astrios保留了我们当前乐器的功能，替换：1）I级生物安全机柜，带有良好的II类机柜； 2）与数字电子产品过时的模拟电子； 3）具有较高灵敏度PMT的过时光电培养基（PMTS）； 4）具有二极管激光器的能量无效的水冷激光器，将能源消耗降低了95％。拟议的astrios还将提供新的激光线（561 nm），并将最大的荧光通道数量从8增加到14。通过我们对珠子和活细胞对Astrios的独立测试，我们已经记录了对现有MOFLO的敏感性，分辨率，排序，排序速度和恢复的显着提高。所需的增强功能将使我们能够继续为我们的大型NIH资助的研究人员提供最佳的单元分类资源。