Bio contained sorter replacement

生物包含分选机更换

• 批准号: 8244762
• 负责人: ALBERT D DONNENBERG
• 金额: $ 59.97万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8244762
• 关键词: Award; Cell Separation; Cells; Chills; Consumption; Custom; Cytometry; Electronics; Engineering; Exercise; Failure; Fluorescence; Funding; Human; Lasers; Life; Pump; Recovery; Research Personnel; Resolution; Resources; Safety; Sampling; Services; Sorting - Cell Movement; Speed; Techniques; Testing; Time; Training; United States National Institutes of Health; Universal Precautions; University of Pittsburgh Cancer Institute; Update; Water; Writing; analog; digital; heat exchanger; instrument; instrumentation; photomultiplier

DESCRIPTION (provided by applicant): This proposal seeks funding to replace the flagship instrument in the University of Pittsburgh Cancer Institute (UPCI) Cytometry Facility (CF), our biocontained High Speed Sorter. The existing instrument, a MoFlo in which the fluidics components are contained within a certified Class I Safety Cabinet, was custom assembled 12 years ago through a Shared Instrumentation Award and $110,000 additional funding from the UPCI. Over its lifetime it has serviced literally hundreds of NIH-funded investigators, including many who make use of the ability to sort live human cells while exercising Universal Precautions. Because of our continuous efforts to update the MoFlo (adding lasers, PMT channels, 4-way and plate sorting, chilled sample station) this instrument has provided excellent service well beyond its expected service life. Despite CF Supervisor E. Michael Meyers' training as a MoFlo field service engineer, the MoFlo has been increasingly out of service (at the time of this writing it has been nonfunctional for 3 weeks due to failure of the obsolete laser water chiller/heat exchanger; the pump is being remanufactured). This places an unacceptable hardship on Facility Users, many of whom require our unique biocontainment capabilities, our expertise in multi-parameter multi-way sorting, and continuity in technique and instrumentation. The newly released Beckman-Coulter Astrios retains the functionality of our current instrument, replacing: 1) The class I biosafety cabinet with a well integrated class II cabinet; 2) Obsolete analog electronics with digital electronics; 3) Obsolete photomultipliers (PMTs) with higher sensitivity PMTs; 4) Energy inefficient water-cooled lasers with diode lasers, reducing energy consumption by 95%. The proposed Astrios will also provide a new laser line (561 nm) and increase the maximum number of fluorescence channels from 8 to 14. Through our own independent testing of the Astrios with beads and live cells, we have documented a significant increase in sensitivity, resolution, sort speed and recovery over our existing MoFlo. The requested enhancements will allow us to continue to provide the best possible cell sorting resource to our large group of NIH-funded investigators.

描述（由申请人提供）：本提案寻求资金，以取代匹兹堡大学癌症研究所（UPCI）细胞仪设备（CF）的旗舰仪器，我们的生物含高速分选机。现有的仪器是MoFlo，其流体元件包含在经过认证的I类安全柜中，是12年前通过共享仪器奖和UPCI提供的11万美元额外资金定制组装的。在它的生命周期中，它已经为数百名美国国立卫生研究院资助的研究人员提供了服务，其中包括许多在行使普遍预防措施的同时利用人类活细胞分类能力的人。由于我们不断努力更新MoFlo（增加激光器，PMT通道，4路和板分选，冷冻样品站），该仪器提供了出色的服务，远远超出其预期的使用寿命。尽管CF主管E. Michael Meyers接受过MoFlo现场服务工程师的培训，但MoFlo已经越来越多地停止使用（在撰写本文时，由于过时的激光冷水机/热交换器故障，MoFlo已经无法工作3周了；泵正在重新制造）。这给设施用户带来了难以接受的困难，他们中的许多人需要我们独特的生物防护能力，我们在多参数多方式分选方面的专业知识，以及技术和仪器的连续性。新发布的Beckman-Coulter Astrios保留了我们现有仪器的功能，取代了：1)I类生物安全柜与集成良好的II类柜；2)用数字电子代替过时的模拟电子；3)采用高灵敏度光电倍增管的过时光电倍增管（pmt）；4)能源效率低下的水冷激光器采用二极管激光器，能耗降低95%。astros还将提供一条新的激光线（561 nm），并将荧光通道的最大数量从8个增加到14个。通过我们自己用珠子和活细胞对astros进行的独立测试，我们记录了与现有的MoFlo相比，灵敏度，分辨率，分选速度和回收率显着提高。所要求的增强功能将使我们能够继续为我们的大型nih资助的研究人员提供最好的细胞分选资源。