IMAGING FLOW CYTOMETER: CELL BIOLOGY

成像流式细胞仪：细胞生物学

• 批准号: 7335086
• 负责人: ALBERT D DONNENBERG
• 金额: $ 7.38万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335086
• 关键词: cell biology; digital imaging; flow cytometry; fluorescence; fluorescent dye /probe; imagery; lead; neoplasm /cancer immunology; play; role

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Amnis ImageStream 100 is the first commercially available imaging flow cytometer. It combines advantages of flow cytometry (ability to interrogate large numbers of cells in suspension, well-developed color compensation, analysis and display methods) with those of image analysis (digital imagery of each individual cell, calculation of morphological features from digital images, localization of fluorescence to morphologic features, co-localization of fluorescent probes). A core group of UPCI investigators has led an initiative to bring this cutting edge technology to the UPCI Flow Facility. Through their efforts, we have the temporary use of the first commercial ImageStream, which was placed in the Facility on a non-capital lease basis. In the few short weeks that we have had access to this instrumentation we have confirmed its capabilities and performed several investigator-initiated experiments described in this proposal. The Facility serves 42 NIH-funded investigators, responsible for over $68 million in NIH-funded research (direct costs). Should this proposal be funded, we are very well positioned to play a leading role in applying this new technology to cancer, immunology, and cell biology research.

该副本是利用NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是赠款，这不一定是调查人员的机构。描述（由申请人提供）：Amnis Imagestream 100是第一个市售的成像流式细胞仪。它结合了流式细胞术的优势（能够在悬浮液中询问大量细胞，发达的色彩补偿，分析和显示方法）与图像分析的优势（每个单独的单个细胞的数字成像，数字图像的计算，荧光定位的形态特征计算，将荧光定位到形态学特征，荧光探针共同定位）。 UPCI调查人员的核心小组领导了一项计划，将这种尖端技术带到UPCI流动设施。通过他们的努力，我们暂时使用了第一个商业成像级，该商业成像级是以非资本租赁为基础放置在设施中的。在我们可以使用该仪器的短短几周内，我们确认了它的功能，并进行了该提案中描述的几个研究者发起的实验。该设施为42名NIH资助的调查人员提供服务，负责超过6800万美元的NIH资助研究（直接费用）。如果该提案得到资助，我们将在将这项新技术应用于癌症，免疫学和细胞生物学研究中发挥主要作用。