IMAGING FLOW CYTOMETER: IMMUNOLOGY

成像流式细胞仪：免疫学

• 批准号: 7335085
• 负责人: ALBERT D DONNENBERG
• 金额: $ 7.38万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335085
• 关键词: cell biology; digital imaging; flow cytometry; fluorescence; fluorescent dye /probe; imagery; immunology; lead; neoplasm /cancer immunology; play; role

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Amnis ImageStream 100 is the first commercially available imaging flow cytometer. It combines advantages of flow cytometry (ability to interrogate large numbers of cells in suspension, well-developed color compensation, analysis and display methods) with those of image analysis (digital imagery of each individual cell, calculation of morphological features from digital images, localization of fluorescence to morphologic features, co-localization of fluorescent probes). A core group of UPCI investigators has led an initiative to bring this cutting edge technology to the UPCI Flow Facility. Through their efforts, we have the temporary use of the first commercial ImageStream, which was placed in the Facility on a non-capital lease basis. In the few short weeks that we have had access to this instrumentation we have confirmed its capabilities and performed several investigator-initiated experiments described in this proposal. The Facility serves 42 NIH-funded investigators, responsible for over $68 million in NIH-funded research (direct costs). Should this proposal be funded, we are very well positioned to play a leading role in applying this new technology to cancer, immunology, and cell biology research.

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：Amnis ImageStream 100是第一款市售成像流式细胞仪。它结合了流式细胞术的优点（能够询问大量悬浮细胞，完善的颜色补偿，分析和显示方法）与图像分析的优点（每个单个细胞的数字图像，从数字图像计算形态特征，荧光定位到形态特征，荧光探针的共定位）。UPCI研究人员的一个核心小组领导了一项倡议，将这种尖端技术带到UPCI流动设施。通过他们的努力，我们暂时使用了第一个商业ImageStream，它以非资本租赁的方式放置在设施中。在短短的几个星期内，我们已经获得了这种仪器，我们已经证实了它的能力，并进行了几个在本提案中描述的闪烁启动实验。该设施为42名NIH资助的研究人员提供服务，负责超过6800万美元的NIH资助的研究（直接成本）。如果这项提案得到资助，我们将非常有能力在将这项新技术应用于癌症，免疫学和细胞生物学研究方面发挥主导作用。