Core--Tissue Resource/Molecular Analysis/Immunopathology

描述（由申请人提供）：组织资源、分子分析和免疫病理学核心机构在收集、处理、储存/储存和分发各种组织和对照品方面拥有丰富的经验，以支持皮肤病研究核心中心（SDRC）成员的研究。可提供新鲜、冷冻和石蜡组织制备物以及未染色的组织载玻片、组织基质阵列、显微解剖组织成分以及组织学服务。核心还可以为研究人员提供难以获得的形态学和分子分析方法 为个别项目提供有效支持。核心提供了光学显微镜和 动物和人类组织和细胞学材料的免疫细胞化学解释，包括检测转染细胞和邻近组织内基因产物的方法。研究人员可以使用通常仅用于人类病理学的复杂技术，包括免疫电子显微镜和特殊组织学服务，如计算机形态测定分析（CAS 200和布利斯系统）和原位杂交。专业诊断病理学家的参与确保了所有检测都是在最佳收集、处理和诊断的组织成分上进行的。集中执行的程序使研究人员免于重复基本工作，从而可以利用现有资源进行更富有成效的工作，并加快实验时间表。核心与生物统计人员密切合作，以便临床/人口统计学信息可以轻松与组织标本联系起来。 核心将支持SDRC的所有项目，其服务将提供给SDRC的所有成员。此外，将免除所有试点项目和收集初步数据以支持赠款申请的费用。视乎对服务的需求及剩余资源而定，现时所要求的资源，可容许向发展检讨委员会成员提供较服务成本低约30%的折扣。

DESCRIPTION (provided by applicant): The Tissue Resource, Molecular Analysis and Immunopathology Core Facility has extensive experience in collecting, processing, storing/banking, and distributing a wide range of tissues and controls to support the research of the Skin Diseases Research Core Center (SDRC) members. Fresh, frozen and paraffin preparations of tissues can be supplied as well as unstained tissue slides, tissue matrix arrays, microdissected tissue components, as well as histology services. The Core also can provide investigators with access to methods of morphological and molecular analysis that are difficult for individual projects to support efficiently. Primarily the Core provides light microscopic and immunocytochemical interpretation of animal and human tissues and cytologic materials including methods to detect gene products within transfected cells and adjacent tissues. Investigators have access to sophisticated techniques usually available only for human pathology, including immunoelectron microscopy and special histology services such as computerized morphometric analysis (CAS200 and BLISS systems) and in situ hybridization. The involvement of dedicated diagnostic pathologists ensures that all assays are performed on optimally collected, processed and diagnosed tissue components. Centrally performed procedures free investigators from duplication of basic work, allowing more productive work with the available resources and acceleration of experimental timetables. The Core works closely with the biostatistical personnel so that clinical/demographic information can be linked easily with tissue specimens. The Core will support all projects of the SDRC and its services will be available to all SDRC members. In addition, its costs will be waived for all pilot projects and for the collection of preliminary data to support grant applications. Depending on demands for services and remaining resources, the current resources requested will permit about a 30% discount below costs of services to SDRC members.