Tissue Resource and Molecular Pathology Core

组织资源和分子病理学核心

• 批准号: 7962145
• 负责人: WILLIAM E. GRIZZLE
• 金额: $ 19.31万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2015-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7962145
• 关键词: Acceleration; African American; Agreement; Alabama; Aliquot; Animal Model; Animals; Area; Authorization documentation; Autopsy; Award; Benign; Biological Assay; Biological Markers; Biopsy; Biostatistics Core; Breast; Cell Line; Cells; Clinical; Clinical Data; Clinical Treatment; Collaborations; Collection; Communication; Consent; Cooperative Human Tissue Network; Databases; Detection; Development; Diagnosis; Diagnostic; Disease; Ensure; Enzyme-Linked Immunosorbent Assay; Equipment and supply inventories; Exocrine pancreas; Extramural Activities; Faculty; Fine needle aspiration biopsy; Flow Cytometry; Free Will; Freezing; Gene Expression; Genes; Head; Health Insurance Portability and Accountability Act; Hepato-Biliary Neoplasm; Hepatobiliary; Histology; Hospitals; Human; Human Pathology; Human Resources; Immunoassay; Immunoelectron Microscopy; Immunohistochemistry; In Situ Hybridization; Individual; Informed Consent; Instruction; Investigational Therapies; Islet Cell Tumor; Laboratories; Laser Microscopy; Lead; Lesion; Light; Link; Liquid substance; Malignant Neoplasms; Malignant neoplasm of pancreas; Mass Spectrum Analysis; Methods; Microdissection; Microscopic; Minnesota; Molecular; Molecular Analysis; Molecular Profiling; Molecular Target; Monitor; National Cancer Institute; Needles; Neoplasms; Nitrogen; Operative Surgical Procedures; Other Genetics; Outcome; Pancreas; Pancreatic Adenocarcinoma; Paraffin; Paraffin Embedding; Pathologic; Pathologist; Pathology; Patients; Phase; Plasma; Preparation; Procedures; Process; Proteins; Protocols documentation; Publishing; Quality Control; Reagent; Recording of previous events; Reproduction spores; Research; Research Ethics Committees; Research Methodology; Research Personnel; Research Project Grants; Research Support; Resources; Saliva; Sampling; Schedule; Secondary to; Serum; Services; Signal Transduction Pathway; Slide; Social Welfare; Solid; Specimen; Staging; Standardization; Surrogate Endpoint; System; Technical Expertise; Techniques; Technology; Terminology; Testing; Time; Tissue Banking; Tissue Banks; Tissue Microarray; Tissue Model; Tissue Sample; Tissues; Transfection; Transgenic Animals; Translational Research; Ultrasonography; Universities; Urine; Work; Xenograft procedure; animal tissue; base; career; chronic pancreatitis; clinical Diagnosis; cost; data management; density; diagnosis standard; effectiveness measure; experience; human disease; human tissue; improved; member; molecular marker; molecular pathology; neoplastic; neoplastic cell; novel; pancreas xenograft; pancreatic neoplasm; programs; quality assurance; repository; success; sulfated glycoprotein 2; tissue preparation; tissue processing; tissue resource; tumor

PROJECT SUMMARY (See instructions): UAB has extensive experience in collecting, processing, storing, and supplying a wide range of human tissues to support research. Fresh, frozen, and paraffin preparations of tissues can be supplied as well as unstained tissue slides and other histology services. We will expand our current tissue resource services as part of the Pancreatic SPORE and join with our partners at the University of Minnesota (UMN) to establish a seamless tissue resource. We will 1) optimize pancreatic tissue collections so that specimens are snap frozen in liquid nitrogen in less than 15 minutes and the number of pancreatic tissues collected are greatly increased; 2) expand our bank of well characterized pancreatic pre-invasive and invasive neoplastic specimens and matching uninvolved specimens from patients who have consented for their tissues to be used in genetic and other research along with clinical data/ outcome; 3) provide microdissected specimens for sensitive technologies requiring pure samples of pancreatic neoplasia; 4) establish uniform collection procedures with UMN and other SPORES 5) develop tissue matrix arrays of samples of pre-invasive and invasive pancreatic neoplasia to permit the concomitant study of many different specimens; and 5) to receive, store and distribute primary pancreatic xenograft cells from the collaboration with Johns Hopkins. This Core also will provide access to methods of morphological analysis that are difficult for individual projects to support efficiently, including quantitative light microscopic and immunocytochemical interpretation of tissues and cytologic materials and will offer methods to detect gene products within transfected cells and adjacent tissues. Investigators will have access to sophisticated techniques usually available only for human pathology, including multiplex immunoassays, tissue microdissection, special histology services, in situ hybridization, confocal laser microscopy, quantitative cytomorphometric analysis and flow cytometry. The core will enable unified purchase, characterization and utilization of shared sets of reagents; centrally and expertly performed procedures will free investigators from duplication of research methods, allowing more productive work with the available resources and acceleration of experimental timetables.

项目总结（见说明）： UAB在收集、处理、储存和供应各种人类生物材料方面拥有丰富的经验。 组织来支持研究。可提供新鲜、冷冻和石蜡组织制剂， 未染色的组织切片和其他组织学服务。我们将扩大我们目前的组织资源服务， 胰腺孢子的一部分，并与明尼苏达大学（UMN）的合作伙伴一起建立一个 无缝组织资源。我们将1）优化胰腺组织的收集， 在液氮中冷冻不到15分钟，收集的胰腺组织数量大大增加， 增加; 2）扩大我们的特征良好的胰腺浸润前和浸润性肿瘤库 样本和匹配的未涉及的样本，这些样本来自同意将其组织 用于遗传学和其他研究沿着临床数据/结果; 3）提供显微解剖标本 对于需要胰腺肿瘤纯样本的敏感技术; 4）建立统一的收集 5）开发侵入前和侵入后的样本的组织基质阵列， 侵袭性胰腺瘤形成，允许同时研究许多不同的标本;和5） 接收、储存和分发与约翰霍普金斯合作的原代胰腺异种移植细胞。 该核心还将提供对个人来说很难的形态分析方法。 有效支持的项目，包括定量光学显微镜和免疫细胞化学解释 并将提供检测转染细胞内基因产物的方法， 邻近组织调查人员将有机会获得通常只适用于人类的复杂技术 病理学，包括多重免疫测定、组织显微切割、特殊组织学服务，原位 杂交、共聚焦激光显微术、定量细胞形态计量分析和流式细胞术。的 核心将能够统一采购、鉴定和使用共用试剂;集中和 熟练地执行程序将使研究人员免于重复研究方法， 利用现有资源开展富有成效的工作，并加快实验时间表。