Tissue Resources and Molecular Pathology Core

组织资源和分子病理学核心

• 批准号: 7677161
• 负责人: WILLIAM E. GRIZZLE
• 金额: $ 12.17万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7677161
• 关键词: Acceleration; Aliquot; Animal Disease Models; Animal Model; Animals; Applications Grants; Authorization documentation; Autopsy; Biological Assay; Biological Markers; Biometry; Cells; Characteristics; Clinical; Clinical Treatment; Collaborations; Collection; Communication; Complex; Core Facility; Data; Databases; Dermatologic; Dermatology; Development; Diagnosis; Diagnostic; Diagnostic Services; Disease; Elements; Ensure; Enzyme-Linked Immunosorbent Assay; Equipment; Equipment and supply inventories; Extramural Activities; Faculty; Feasibility Studies; Flow Cytometry; Freezing; Gene Chips; Genes; Goals; Health Insurance Portability and Accountability Act; Histology; Human; Human Pathology; Human Resources; Immunoassay; Immunoelectron Microscopy; Immunohistochemistry; In Situ Hybridization; Individual; Informed Consent; Investigational Therapies; Laboratories; Lasers; Lesion; Light; Link; Methods; Microdissection; Microscopic; Molecular Analysis; Molecular Target; Monitor; Northern Blotting; Operative Surgical Procedures; Other Genetics; Outcome; Paraffin; Pathologic; Pathologist; Pathology; Patients; Phase; Physiology; Pilot Projects; Preparation; Prevention strategy; Privacy; Procedures; Process; Proteins; Protocols documentation; Publishing; Quality Control; Radical Mastectomy; Reagent; Recording of previous events; Research; Research Ethics Committees; Research Personnel; Research Project Grants; Research Support; Resources; Rodent; Sampling; Schedule; Services; Skin; Skin Tissue; Slide; Social Welfare; Source; Specimen; Standardization; Surrogate Endpoint; System; Technical Expertise; Techniques; Technology; Test Result; Testing; Therapeutic; Time; Tissue Banking; Tissue Banks; Tissue Microarray; Tissue Model; Tissue Sample; Tissues; Training; Transfection; Translational Research; Work; analytical method; animal tissue; base; computerized; cost; data management; design; discount; disorder control; effectiveness measure; experience; experimental analysis; human disease; human tissue; imaging modality; improved; in vivo; member; molecular marker; molecular pathology; novel; programs; prospective; quality assurance; skills; skin disorder; skin lesion; success; tissue preparation; tissue processing; tissue resource

The Tissue Resource and Molecular Pathology Core Facility has extensive experience in collecting, processing, storing/banking, and distributing a wide range of tissues and controls to support the research of the Skin Diseases Research Center (SDRC) members. Fresh, frozen and paraffin preparations of tissues can be supplied as well as unstained tissue slides, tissue matrix arrays, microdissected tissue components, as well as histology services. The Core also can provide investigators with access to methods of morphological and molecular analysis that are difficult for individual projects to support efficiently. Primarily the Core provides light micrscopic and immunocytochemical interpretation of animal and human tissues and cytologic materials including methods to detect gene products within transfected cells and adjacent tissue. Investigators have access to sophisticated techniques usually available only for human pathology, including immunoelectron microscopy and special histology services such as computerized morphometric analysis (CAS200 and BLISS) systems, in situ hybridization, and flow cytometry. Analytical methods available to the SDRC include high throughput ELISA assays and Western, Southern and Northern blotting. Both multiplex RT-Q-PCR and immunoassays are also available. The involvement of dedicated diagnostic pathologists ensures that all assays are performed on optimally collected, processed and diagnosed tissue components. Centrally performed procedures free investigators from duplication of basic work, allowing more productive work with the available resources and acceleration of experimental timetables. The Core works closely with biostatistical personnel so that clinical/demographic information can be linked easily with tissue specimens. The Core will support all projects of the SDRC and its services will be available to all SDRC members. In addition, its costs will be waived for Pilot and Feasibility Studies, as appropriate, for the collection of preliminary data to support grant applications. Depending on demands for services, the current resources requested will permit about a 50% discount below costs of services to SDRC members.

组织资源和分子病理学核心设施在收集，处理， 存储/银行，并分发广泛的组织和控制，以支持皮肤的研究 疾病研究中心（SDRC）成员。可提供新鲜、冷冻和石蜡组织制剂 以及未染色的组织载玻片、组织基质阵列、显微解剖的组织成分以及组织学 服务核心还可以为研究人员提供形态学和分子生物学方法， 这些分析很难为单个项目提供有效支持。核心提供了光， 动物和人体组织及细胞学材料的显微镜和免疫细胞化学解释 包括检测转染细胞和邻近组织中基因产物的方法。调查人员 获得通常仅用于人类病理学的复杂技术，包括免疫电子 显微镜和特殊组织学服务，如计算机形态分析（CAS 200和布利斯） 系统、原位杂交和流式细胞术。SDRC可用的分析方法包括高 通量ELISA测定和Western、Southern和北方印迹。多重RT-Q-PCR和 免疫测定也是可用的。专职诊断病理学家的参与确保了所有检测 在最佳收集、处理和诊断的组织成分上进行。中心执行 程序使调查人员免于重复基本工作， 资源和加快实验时间表。核心与生物统计人员密切合作， 临床/人口统计信息可以轻松与组织样本联系起来。核心将支持所有 所有成员均可使用该中心的项目和服务。此外，其费用将被免除 进行试验和可行性研究，以收集初步数据， 应用.根据对服务的需求，目前所要求的资源将允许大约50%的 折扣低于SDRC成员的服务成本。