Tissue Resources/Immunopathology/Molecular Assays

组织资源/免疫病理学/分子测定

• 批准号: 7290720
• 负责人: WILLIAM E. GRIZZLE
• 金额: $ 28.72万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7290720
• 关键词: Acceleration; African American; Aliquot; Animal Model; Animals; Antibodies; Area; Authorization documentation; Autopsy; Benign; Biological Assay; Biological Markers; Biostatistics Core; Brain; Breast; Breast Diseases; Breast Fibrocystic Disease; Cancer Control; Carcinoma; Cell Line; Cells; Clinical; Clinical Treatment; Code; Collaborations; Collection; Combined Modality Therapy; Communication; Complex; Cooperative Human Tissue Network; Core Facility; Databases; Development; Diagnosis; Diagnostic; Diagnostic Services; Disease; Disseminated Malignant Neoplasm; Electrophoresis; Ensure; Enzyme-Linked Immunosorbent Assay; Equipment and supply inventories; Extramural Activities; Flow Cytometry; Freezing; Goals; Grant; Health Insurance Portability and Accountability Act; Health Services Research; Histology; Hospitals; Human; Human Resources; Immune; Immunoassay; Immunoelectron Microscopy; Immunohistochemistry; In Situ Hybridization; Individual; Informed Consent; Invasive; Investigational Therapies; Laboratories; Lesion; Light; Link; Malignant Neoplasms; Mammary Gland Parenchyma; Mammary Neoplasms; Mass Spectrum Analysis; Measures; Metastatic Lesion; Methods; Microdissection; Microscopic; Molecular; Molecular Target; Monitor; Neoplasms; Noninfiltrating Intraductal Carcinoma; Other Genetics; Outcome; Ovarian; Pancreas; Paraffin; Pathologic; Pathology; Patients; Phase; Physiology; Pilot Projects; Polymerase Chain Reaction; Procedures; Process; Proteins; Proteomics; Protocols documentation; Publishing; Purpose; Quality Control; Range; Reagent; Recording of previous events; Reproduction spores; Research; Research Ethics Committees; Research Personnel; Research Project Grants; Resected; Resources; Rodent; Rodent Model; Sampling; Schedule; Serum; Services; Social Welfare; Solid; Specimen; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Standardization; Standards of Weights and Measures; Support of Research; Surrogate Endpoint; System; Technical Expertise; Techniques; Technology; Testing; Tissue Banks; Tissue Microarray; Tissue Model; Tissue Sample; Tissues; Tumor Bank; Urine; Work; animal tissue; anticancer research; base; breast lesion; career; computerized; cost; data management; experience; human disease; human tissue; immunopathology; improved; malignant breast neoplasm; member; molecular pathology; neoplastic; novel; outcome forecast; programs; quality assurance; reagent standard; repository; tissue preparation; tissue processing; tissue resource; tumor; virtual

UAB has extensive experience in collecting, processing, storing, and supplying a wide range of human breast tissues to support Breast SPORE research. Fresh, frozen, and paraffin preparations of tissues can be supplied as well as histology services. The Breast SPORE 1) has established a bank of well characterized breast tumor specimens and matching uninvolved specimens from patients who have given specific informed consent for their tissues to be used in genetic and other types of research. Through October 2005, the Core has obtained research specimens from 1,168 patients with surgically resected diseases of the breast; over 4,000 specimens of breast or related tissues, including about 1,500 specimens of breast neoplasia, have been stored in a bank of frozen solid tissues; 2) has processed to paraffin blocks over 3,000 breast specimens, including 1,300 carcinoma specimens. Tissue arrays have been constructed from these blocks as well as from diagnostic paraffin blocks; 3) has provided frozen, paraffin processed and/or microdissected specimens for SPORE intramural as well as extramural investigators. The Core also offers investigators access to multiple experimental methods including light microscopic and immunocytochemical interpretation of animal and human tissues and cytologic materials and methods to detect gene products within cells and adjacent tissues. Investigators have access to techniques usually available only for human diagnostic pathology, including tissue microdissection, tissue arrays, antibody arrays, immunoelectron microscopy, computerized cytomorphometric analysis, special histology services, in situ hybridization, proteomic techniques including multidimensional electrophoresis, ELISA, multiplex immunoassay (Luminex), SELDI/MALDI-mass spectroscopy and flow cytometry. The Core permits the utilization of a shared set of reagents and centrally performed procedures that permits cross project comparisons of research on the same cases of breast cancer. Investigators are freed from duplication of basic work, allowing more productive work with the available resources and acceleration of experimental timetables. In summary, this Core aids investigators to reach their goals in breast cancer research; specifically, it provides samples of human tissues needed to complete research projects and aids in the research by providing methodological support for the research of SPORE investigators.

UAB在收集、加工、储存和供应各种人力资源方面拥有丰富的经验 支持乳房孢子研究的乳房组织。新鲜的、冷冻的和石蜡组织制剂可以 以及组织学服务。乳孢子筑起了一道水井 具有特征性的乳腺肿瘤标本和匹配的来自曾接受过 对其组织用于遗传和其他类型研究的具体知情同意。穿过 2005年10月，Core从1,168名手术切除的患者中获得了研究样本 乳房疾病；4,000多个乳房或相关组织标本，包括约1,500个标本 乳腺肿瘤，已储存在银行的冰冻固体组织；2)已加工成石蜡切片 超过3,000个乳房标本，包括1,300个癌标本。已经构建了组织阵列 从这些块以及从诊断石蜡块；3)提供了冷冻、石蜡加工 和/或供孢子壁内和壁外调查人员使用的显微解剖标本。核心也是 为研究人员提供多种实验方法，包括光学显微镜和 动物和人体组织和细胞学材料的免疫细胞化学解释和方法 检测细胞和邻近组织中的基因产物。调查人员通常可以接触到技术 仅适用于人类诊断病理学，包括组织显微切割、组织阵列、抗体 阵列、免疫电子显微镜、计算机细胞形态计量学分析、特殊组织学服务 原位杂交、蛋白质组学技术，包括多维电泳、酶联免疫吸附试验、多重 免疫分析(Luminex)、SELDI/MALDI-MS和流式细胞术。核心允许 利用一套共享的试剂和集中执行的程序，允许跨项目 对相同乳腺癌病例的研究进行比较。调查人员不再重复 基础工作，让现有资源更有成效地工作，并加快实验 时间表。 总而言之，这个核心帮助研究人员达到他们在乳腺癌研究中的目标；具体地说，它 提供完成研究项目所需的人体组织样本，并通过 为孢子调查者的研究提供方法论支持。