Mass and NMR Spectroscopy Core Facility

质量和核磁共振波谱设备核心为烧伤创伤中心提供了应用质谱学和核磁共振(核磁共振)波谱技术来研究组织、细胞和遗传水平上的现象的能力。质谱仪支持的研究允许我们的研究人员将示踪技术应用于我们的研究中的以下应用：氨基酸代谢、葡萄糖代谢、脂肪代谢、硝酸盐/一氧化氮、蛋白质周转、蛋白质磷酸化和底物动力学/氧化剂。质谱学设施为烧伤创伤中心提供多项服务。(1)提供对体液、组织和呼出空气中示踪剂及其衍生物的稳定同位素丰度的及时和准确的测定。(2)协助开发血液、组织和尿液中化合物的同位素分析新方法，以满足我们研究人员的需要。(3)为支持使用稳定核素作为示踪剂的研究研究而进行的所有分析提供质量控制。核磁共振波谱设施支持研究，以开发方法，使我们的 研究人员在组织提取物中评估TCA循环中的关键代谢物，并使用磁角旋转，在整个组织中。此外，研究人员将能够使用C13/p31核磁共振评估体内线粒体能量耦合，如ATP合成与TCA循环的比率所表示的。最后，研究人员将能够评估线粒体2-氧杂多酸-苹果酸载体，特别是因为线粒体膜上没有NADH的运输，糖酵解在胞浆中产生的还原等价物以苹果酸的形式进入线粒体。核磁共振波谱设备为烧伤创伤中心提供多项服务。(1)对人体糖异生和TCA循环通量的无创性评估。(2)利用现有技术和新技术对组织提取液进行核磁共振。(3)开发使用高分辨率磁角旋转在小的、完整的、未处理的组织中进行核磁共振测量的新技术。(4)小鼠烧伤模型的体内核磁共振波谱和磁共振成像显微镜(MRI)。这些设施使所有4个项目的研究成为可能，每个项目都包括直接支持这些活动的资金。要求为方法和技术开发提供人员和用品的支助。

The Mass and NMR Spectroscopy Facility Core provides the Burn Trauma Center with the capability to apply mass spectroscopy and nuclear magnetic resonance (NMR) spectroscopy techniques to study phenomena at the tissue, cellular, and genetic levels. The Mass Spectroscopy Facility supports research that allows our investigators to apply tracer techniques for the following applications in our research: amino acid metabolism, glucose metabolism, lipid metabolism, nitrate/nitric oxide, protein turnover, protein phosphorylation, and substrate kinetics/oxidaticn. The Mass Spectroscopy Facility offers several services to the Burn Trauma Center. (1) Provides timely and accurate determinations of stable isotope enrichments of tracers and their derivatives in bodily fluids, tissues, and expired air. (2) Assists in the development of new methods for isotope analyses of compounds in blood, tissues, and urine as required to meet the needs of our investigators. (3) Provides quality control for all analysis conducted in support of the research studies that involve the use of stable nuclides as tracers. The NMR Spectroscopy Facility supports research to develop methodologies that will permit our investigators to assess key metabolites in the TCA Cycle in tissue extracts and using magnetic angle spinning, in the whole tissue. In addition, the investigators will be able to assess mitochondrial energy coupling in vivo, as expressed by the ratio of ATP synthesis to TCA cycle, using C13/P31 NMR. Finally, the investigators will be able to assess the mitochondrial 2-oxogulterate-malate carrier especially since there is no transport of NADH across the mitochondrial membrane and reducing equivalents produced in the cytosol from glycolysis are carried into the mitochondria as malate. The NMR Spectroscopy Facility offers several services to the Burn Trauma Center. (1) Noninvasive assessment of gluconeogenesis and TCA cycle flux in humans. (2) NMR of tissue extracts using existing and new techniques. (3) Development of new technologies using high resolution magnetic angle spinning to perform NMR measurements in small, intact, unprocessed tissues. (4) In vivo NMR spectroscopy and MR imaging microscopy (MRI) of murine models of burn injury. These facilities enable research in all 4 projects and funds to support these activities directly are included in each project. Support is requested for personnel and supplies for methodology and technology development.