Mass and NMR Spectroscopy Core Facility

质量和核磁共振波谱核心设施

• 批准号: 7599237
• 负责人: VERNON L YOUNG
• 金额: $ 13.86万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7599237
• 关键词: ATP Synthesis Pathway; Air; Amino Acids; Blood; Burn Trauma; Burn injury; Citric Acid Cycle; Core Facility; Coupling; Cytosol; Development; Funding; Genetic; Gluconeogenesis; Glycolysis; Human; Human Resources; Isotopes; Kinetics; Liquid substance; Magnetic Resonance Imaging; Magnetism; Malates; Mass Spectrum Analysis; Measurement; Metabolism; Methodology; Methods; Microscopy; Mitochondria; Modeling; Mus; NADH; NMR Spectroscopy; Nitrates; Nitric Oxide; Nuclear Magnetic Resonance; Phosphorylation; Quality Control; Research; Research Personnel; Resolution; Services; Support of Research; Techniques; Tissue Extracts; Tissues; Tracer; Urine; glucose metabolism; in vivo; lipid metabolism; malate; mitochondrial membrane; new technology; nitrate; protein degradation; research study; stable isotope; technology development; trauma centers

The Mass and NMR Spectroscopy Facility Core provides the Burn Trauma Center with the capability to apply mass spectroscopy and nuclear magnetic resonance (NMR) spectroscopy techniques to study phenomena at the tissue, cellular, and genetic levels. The Mass Spectroscopy Facility supports research that allows our investigators to apply tracer techniques for the following applications in our research: amino acid metabolism, glucose metabolism, lipid metabolism, nitrate/nitric oxide, protein turnover, protein phosphorylation, and substrate kinetics/oxidaticn. The Mass Spectroscopy Facility offers several services to the Burn Trauma Center. (1) Provides timely and accurate determinations of stable isotope enrichments of tracers and their derivatives in bodily fluids, tissues, and expired air. (2) Assists in the development of new methods for isotope analyses of compounds in blood, tissues, and urine as required to meet the needs of our investigators. (3) Provides quality control for all analysis conducted in support of the research studies that involve the use of stable nuclides as tracers. The NMR Spectroscopy Facility supports research to develop methodologies that will permit our investigators to assess key metabolites in the TCA Cycle in tissue extracts and using magnetic angle spinning, in the whole tissue. In addition, the investigators will be able to assess mitochondrial energy coupling in vivo, as expressed by the ratio of ATP synthesis to TCA cycle, using C13/P31 NMR. Finally, the investigators will be able to assess the mitochondrial 2-oxogulterate-malate carrier especially since there is no transport of NADH across the mitochondrial membrane and reducing equivalents produced in the cytosol from glycolysis are carried into the mitochondria as malate. The NMR Spectroscopy Facility offers several services to the Burn Trauma Center. (1) Noninvasive assessment of gluconeogenesis and TCA cycle flux in humans. (2) NMR of tissue extracts using existing and new techniques. (3) Development of new technologies using high resolution magnetic angle spinning to perform NMR measurements in small, intact, unprocessed tissues. (4) In vivo NMR spectroscopy and MR imaging microscopy (MRI) of murine models of burn injury. These facilities enable research in all 4 projects and funds to support these activities directly are included in each project. Support is requested for personnel and supplies for methodology and technology development.

质谱和核磁共振光谱设施核心使烧伤中心能够应用质谱和核磁共振 (NMR) 光谱技术来研究组织、细胞和遗传水平的现象。质谱设施支持的研究使我们的研究人员能够将示踪技术应用于我们的研究中的以下应用：氨基酸代谢、葡萄糖代谢、脂质代谢、硝酸盐/一氧化氮、蛋白质周转、蛋白质磷酸化和底物动力学/氧化。质谱设施为烧伤创伤中心提供多种服务。 (1) 及时准确地测定体液、组织和呼出气体中示踪剂及其衍生物的稳定同位素富集度。 (2) 协助开发血液、组织和尿液中化合物同位素分析的新方法，以满足我们研究人员的需求。 (3) 为支持涉及使用稳定核素作为示踪剂的研究而进行的所有分析提供质量控制。核磁共振波谱学设施支持研究开发方法，使我们能够 研究人员评估组织提取物中 TCA 循环中的关键代谢物，并使用磁角旋转在整个组织中进行评估。此外，研究人员将能够使用 C13/P31 NMR 评估体内线粒体能量耦合，以 ATP 合成与 TCA 循环的比率表示。最后，研究人员将能够评估线粒体 2-氧代-苹果酸载体，特别是因为 NADH 没有穿过线粒体膜的运输，并且糖酵解在胞质溶胶中产生的还原当量以苹果酸的形式被携带到线粒体中。核磁共振波谱仪为烧伤创伤中心提供多种服务。 (1) 人体糖异生和 TCA 循环通量的无创评估。 (2) 使用现有技术和新技术对组织提取物进行核磁共振。 (3) 开发新技术，利用高分辨率磁角旋转在小型、完整、未处理的组织中进行 NMR 测量。 (4)烧伤小鼠模型的体内核磁共振波谱和磁共振成像显微镜(MRI)。这些设施支持所有 4 个项目的研究，每个项目都包含直接支持这些活动的资金。需要为方法和技术开发提供人员和物资支持。