Mass and NMR Spectroscopy Core Facility

质量和核磁共振光谱学设施核心为烧伤创伤中心提供了应用质谱和核磁共振（NMR）光谱学技术来研究组织、细胞和遗传水平现象的能力。质谱设备支持研究，允许我们的研究人员在我们的研究中应用示踪技术：氨基酸代谢，葡萄糖代谢，脂质代谢，硝酸盐/一氧化氮，蛋白质周转，蛋白质磷酸化和底物动力学/氧化蛋白。质谱设施为烧伤创伤中心提供多项服务。(1)及时、准确地测定体液、组织和过期空气中示踪剂及其衍生物的稳定同位素丰度。(2)协助开发血液、组织和尿液中化合物同位素分析的新方法，以满足研究人员的需要。(3)为支持使用稳定核素作为示踪剂的研究而进行的所有分析提供质量控制。核磁共振光谱学设施支持研究开发的方法，将允许我们的

The Mass and NMR Spectroscopy Facility Core provides the Burn Trauma Center with the capability to apply mass spectroscopy and nuclear magnetic resonance (NMR) spectroscopy techniques to study phenomena at the tissue, cellular, and genetic levels. The Mass Spectroscopy Facility supports research that allows our investigators to apply tracer techniques for the following applications in our research: amino acid metabolism, glucose metabolism, lipid metabolism, nitrate/nitric oxide, protein turnover, protein phosphorylation, and substrate kinetics/oxidaticn. The Mass Spectroscopy Facility offers several services to the Burn Trauma Center. (1) Provides timely and accurate determinations of stable isotope enrichments of tracers and their derivatives in bodily fluids, tissues, and expired air. (2) Assists in the development of new methods for isotope analyses of compounds in blood, tissues, and urine as required to meet the needs of our investigators. (3) Provides quality control for all analysis conducted in support of the research studies that involve the use of stable nuclides as tracers. The NMR Spectroscopy Facility supports research to develop methodologies that will permit our investigators to assess key metabolites in the TCA Cycle in tissue extracts and using magnetic angle spinning, in the whole tissue. In addition, the investigators will be able to assess mitochondrial energy coupling in vivo, as expressed by the ratio of ATP synthesis to TCA cycle, using C13/P31 NMR. Finally, the investigators will be able to assess the mitochondrial 2-oxogulterate-malate carrier especially since there is no transport of NADH across the mitochondrial membrane and reducing equivalents produced in the cytosol from glycolysis are carried into the mitochondria as malate. The NMR Spectroscopy Facility offers several services to the Burn Trauma Center. (1) Noninvasive assessment of gluconeogenesis and TCA cycle flux in humans. (2) NMR of tissue extracts using existing and new techniques. (3) Development of new technologies using high resolution magnetic angle spinning to perform NMR measurements in small, intact, unprocessed tissues. (4) In vivo NMR spectroscopy and MR imaging microscopy (MRI) of murine models of burn injury. These facilities enable research in all 4 projects and funds to support these activities directly are included in each project. Support is requested for personnel and supplies for methodology and technology development.