Integration of high-multiplex imaging with spatial transcriptomics and single-cell RNA-sequencing applied to the tumour microenvironment.

将高多重成像与空间转录组学和单细胞 RNA 测序应用于肿瘤微环境。

• 批准号: 2391784
• 金额: --
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2391784
• 关键词: Integration; multiplex; imaging; spatial; transcriptomics

This project aims to utilise high-plex immunofluorescence imaging, spatial transcriptomics and scRNA-seq data to investigate cancer pathophysiology. Investigating the interface between tumour cells and the tumour microenvironment by spatially resolving gene and protein expression in neighbouring cells. High-plex immunofluorescence imaging will also be used to develop a method for identifying direct cell-cell contact and quantifying protein expression that forms putative juxtracrine signalling with the aim of quantifying key regulators at the tumour microenvironment. Finally, the project seeks to integrate the various expression data into a high-dimensional multi-omics dataset.

该项目旨在利用高重免疫荧光成像，空间转录组学和scRNA-seq数据来研究癌症病理生理学。通过空间分辨相邻细胞中的基因和蛋白质表达来研究肿瘤细胞和肿瘤微环境之间的界面。高重免疫荧光成像也将用于开发一种方法，用于识别直接的细胞-细胞接触和定量蛋白质表达，形成推定的促肾上腺素信号传导，目的是量化肿瘤微环境中的关键调节因子。最后，该项目旨在将各种表达数据整合到一个高维多组学数据集中。