MINORITY PREDOCTORAL FELLOWSHIP PROGRAM

描述（由申请人提供）：反式高尔基体网络（TGN）和内体是分选和运输各种生物合成和内吞蛋白的主要位点，包括参与胆固醇稳态、病毒感染和细胞生长调节的因子。TGN和内体之间的蛋白质转运通过被主要由网格蛋白和衔接子AP-1和Gga组成的外壳包围的囊泡发生。虽然这些关键的外壳组件已被充分研究，我们的知识所需的辅助因子，以调节网格蛋白介导的TGN和内体之间的运输是不完全的。本研究将表征酿酒酵母的进化上保守的推定网格蛋白辅助蛋白，其似乎在AP-1介导的运输中特异性地起作用。它的功能和蛋白质运输的作用将通过检查网格蛋白介导的运输缺乏蛋白质的细胞和其他辅助因素将被确定在屏幕上的结合伙伴。这些研究将促进我们对TGN和内体之间蛋白质转运机制的理解，并有助于区分目前尚未解决的AP-1和Gga介导的途径。

DESCRIPTION (provided by applicant): The trans-Golgi network (TGN) and endosomes are major sites for sorting and trafficking of a variety of biosynthetic and endocytic proteins, including factors that are involved in cholesterol homeostasis, virus infection, and cell growth regulation. Protein transport between the TGN and endosomes occurs via vesicles surrounded by coats composed mainly of clathrin and the adaptors AP-1 and Gga. Though these key coat components have been well-studied, our knowledge about accessory factors that are needed to regulate clathrin-mediated transport between the TGN and endosomes is less complete. This study will characterize an evolutionarily conserved putative clathrin accessory protein of Saccharomyces cerevisiae that appears to act specifically in AP-1 mediated transport. Its function and role in protein trafficking will be addressed by examining clathrin-mediated transport in cells lacking the protein and additional accessory factors will be identified in screens for binding partners. These studies will advance our understanding of the mechanisms of protein transport between the TGN and endosomes and help distinguish between the currently unresolved AP-1 and Gga-mediated pathways.