MINORITY PREDOCTORAL FELLOWSHIP PROGRAM

少数族裔博士前奖学金计划

• 批准号: 6951841
• 负责人: Gerardo Esteban Fernandez
• 金额: $ 2.92万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6951841
• 关键词: AP1 protein; Golgi apparatus; Saccharomyces cerevisiae; clathrin; endocytosis; predoctoral investigator; protein binding; protein localization; protein protein interaction; protein structure function; protein transport; vesicle /vacuole

DESCRIPTION (provided by applicant): The trans-Golgi network (TGN) and endosomes are major sites for sorting and trafficking of a variety of biosynthetic and endocytic proteins, including factors that are involved in cholesterol homeostasis, virus infection, and cell growth regulation. Protein transport between the TGN and endosomes occurs via vesicles surrounded by coats composed mainly of clathrin and the adaptors AP-1 and Gga. Though these key coat components have been well-studied, our knowledge about accessory factors that are needed to regulate clathrin-mediated transport between the TGN and endosomes is less complete. This study will characterize an evolutionarily conserved putative clathrin accessory protein of Saccharomyces cerevisiae that appears to act specifically in AP-1 mediated transport. Its function and role in protein trafficking will be addressed by examining clathrin-mediated transport in cells lacking the protein and additional accessory factors will be identified in screens for binding partners. These studies will advance our understanding of the mechanisms of protein transport between the TGN and endosomes and help distinguish between the currently unresolved AP-1 and Gga-mediated pathways.

描述(申请人提供)：反式高尔基体网络(TGN)和内小体是分类和运输各种生物合成和内吞蛋白的主要场所，包括参与胆固醇稳态、病毒感染和细胞生长调节的因子。TGN和内体之间的蛋白质运输是通过囊泡进行的，囊泡被主要由网状蛋白和接头AP-1和GGA组成的涂层所包围。虽然这些关键的被膜成分已经得到了很好的研究，但我们对调节TGN和内体之间的网状蛋白介导的运输所需的辅助因子的了解还不够全面。这项研究将描述酿酒酵母中一种进化上保守的可能的笼状蛋白辅助蛋白，它似乎在AP-1介导的运输中发挥特殊作用。它在蛋白质运输中的功能和作用将通过检测缺乏蛋白质的细胞中的网状蛋白介导的运输来解决，并将在结合伙伴的筛选中确定其他辅助因子。这些研究将促进我们对TGN和内小体之间的蛋白质运输机制的理解，并有助于区分目前尚未解决的AP-1和GGA介导的途径。