Confocal Microscope

项目摘要 洛杉矶儿童医院（CHLA）申请资金购买Leica STELLARIS 5共焦 显微镜将被安置在萨班研究所已建立的细胞成像核心设施 （TSRI），美国为数不多的独立研究中心之一，科学探究与 临床护理，专门用于儿童。我们的研究人员依靠共聚焦显微镜来探测 细胞和分子机制的发展和疾病，跨越器官系统，在不同的 儿科人群。这一请求有很强的理由。首先，有两个蔡司7系列共焦 Cellular Imaging Core中的显微镜（LSM 710和700）已经使用了10年以上，技术上已经过时， 并且接近其使用寿命结束;蔡司将在两年内停止为它们提供服务合同。 其次，LSM 700只有2个通道，采用过时的检测技术，机械性能非常差， 慢了第三，LSM 710变得不可靠，非功能时间大大增加。后 LSM 710的一系列总唐斯威胁到正在进行的研究项目，我们能够 获得机构支持，以获得Leica STELLARIS 5共聚焦;安装于2020年12月。新 仪器迎来了下一代技术的细胞成像核心，导致高需求 以及我们多元化用户群的高满意度。单靠它自己，单一的恒星是没有能力 支持我们的用户所需的每年3000多小时的共聚焦成像，以推进他们的NIH资助的 项目，也无法支持正在招募到CHLA的额外调查人员，并将需要 技术，建立自己的独立研究计划。因此，该机构提供了支持， 最初的STELLARIS，但在购买第二个STELLARIS 5共焦之前， 蔡司装置变得不能使用和过时。在详细测试了4个共焦模型（徕卡，尼康， Olympus和Zeiss），我们确定STELLARIS 5是CHLA研究者的最佳选择， 由于其4个灵敏的混合光探测器（HyD S），每个都是完全可调的， 400-850 nm范围内的1 nm精度。这也是最佳的配合，因为两个STELLARIS 5的一致性 单元最大限度地提高了用户组在培训、操作和安排仪器时间方面的效率。在 细胞成像核心，要求STELLARIS 5将完全由CHLA提供财政支持， 由一位经验丰富的博士级显微镜专家管理。该仪器将极大地有利于整个共焦 通过比两个旧系统生成更多成像数据的能力， 让我们能够实现阐明器官如何发育以及异常结构如何 导致儿科疾病。

PROJECT ABSTRACT Children’s Hospital Los Angeles (CHLA) requests funds to purchase a Leica STELLARIS 5 confocal microscope to be housed in the established Cellular Imaging Core Facility of The Saban Research Institute (TSRI), one of the few freestanding research centers in the U.S. where scientific inquiry is combined with clinical care and is devoted exclusively to children. Our investigators rely on confocal microscopy to probe the cellular and molecular mechanisms underlying development and disease, spanning organ systems, in diverse pediatric populations. There is a strong rationale for the request. First, there are two Zeiss 7-series confocal microscopes (LSM 710 and 700) in the Cellular Imaging Core that are over 10 years old, technically outdated, and near the end of their serviceable life; Zeiss will cease to offer service contracts for them within 2 years. Second, the LSM 700 only has 2 channels, utilizes outmoded detection technology, and is mechanically very slow. Third, the LSM 710 has become unreliable and nonfunctional time has increased considerably. After a series of total break downs of the LSM 710, which threatened ongoing research projects, we were able to garner institutional support to acquire a Leica STELLARIS 5 confocal; installed in December 2020. The new instrument ushered in a next generation of technology to the Cellular Imaging Core, resulting in high demand and high satisfaction across our diverse user base. On its own, the single STELLARIS is not capable of supporting the 3000-plus annual hours of confocal imaging required by our users to advance their NIH-funded projects, nor is it able to support additional investigators who are being recruited to CHLA and will require this technology to establish their independent research programs. Thus, the institution has provided the support for the initial STELLARIS, but is in significant need of support to purchase a second STELLARIS 5 confocal before the Zeiss units become unserviceable and obsolete. After detailed testing of 4 confocal models (Leica, Nikon, Olympus, and Zeiss), we determined that the STELLARIS 5 is optimal for CHLA investigators with fixed- specimen applications because of its 4 sensitive hybrid light detectors (HyD S) that are each fully tunable with 1-nm precision in the range of 400-850 nm. It is also the best fit because the consistency of two STELLARIS 5 units maximizes efficiency for the user group in training, operating, and scheduling time on the instruments. In the Cellular Imaging Core, the requested STELLARIS 5 will be fully supported financially by CHLA and managed by an experienced PhD-level microscopist. This instrument will greatly benefit the entire confocal user base at CHLA through the ability to generate more imaging data than either of the two old systems, allowing us to meet the long-term objective of illuminating how organs develop and how aberrant structures lead to pediatric disease.