Confocal Microscope

共焦显微镜

• 批准号: 10415816
• 负责人: Gerardo Esteban Fernandez
• 金额: $ 24.99万
• 依托单位: CHILDREN'S HOSPITAL OF LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10415816
• 关键词: 10 year old; Child; Childhood; Confocal Microscopy; Contract Services; Core Facility; Data; Detection; Development; Disease; Doctor of Philosophy; Financial Support; Funding; Hour; Hybrids; Image; Institution; Lead; Life; Light; Los Angeles; Mechanics; Microscope; Modeling; Molecular; Organ; Pediatric Hospitals; Population; Research; Research Institute; Research Personnel; Research Project Grants; Schedule; Scientific Inquiry; Series; Services; Specimen; Structure; System; Technology; Testing; Time; Training; United States National Institutes of Health; base; body system; cellular imaging; clinical care; confocal imaging; detector; experience; instrument; next generation; programs; recruit; satisfaction

PROJECT ABSTRACT Children’s Hospital Los Angeles (CHLA) requests funds to purchase a Leica STELLARIS 5 confocal microscope to be housed in the established Cellular Imaging Core Facility of The Saban Research Institute (TSRI), one of the few freestanding research centers in the U.S. where scientific inquiry is combined with clinical care and is devoted exclusively to children. Our investigators rely on confocal microscopy to probe the cellular and molecular mechanisms underlying development and disease, spanning organ systems, in diverse pediatric populations. There is a strong rationale for the request. First, there are two Zeiss 7-series confocal microscopes (LSM 710 and 700) in the Cellular Imaging Core that are over 10 years old, technically outdated, and near the end of their serviceable life; Zeiss will cease to offer service contracts for them within 2 years. Second, the LSM 700 only has 2 channels, utilizes outmoded detection technology, and is mechanically very slow. Third, the LSM 710 has become unreliable and nonfunctional time has increased considerably. After a series of total break downs of the LSM 710, which threatened ongoing research projects, we were able to garner institutional support to acquire a Leica STELLARIS 5 confocal; installed in December 2020. The new instrument ushered in a next generation of technology to the Cellular Imaging Core, resulting in high demand and high satisfaction across our diverse user base. On its own, the single STELLARIS is not capable of supporting the 3000-plus annual hours of confocal imaging required by our users to advance their NIH-funded projects, nor is it able to support additional investigators who are being recruited to CHLA and will require this technology to establish their independent research programs. Thus, the institution has provided the support for the initial STELLARIS, but is in significant need of support to purchase a second STELLARIS 5 confocal before the Zeiss units become unserviceable and obsolete. After detailed testing of 4 confocal models (Leica, Nikon, Olympus, and Zeiss), we determined that the STELLARIS 5 is optimal for CHLA investigators with fixed- specimen applications because of its 4 sensitive hybrid light detectors (HyD S) that are each fully tunable with 1-nm precision in the range of 400-850 nm. It is also the best fit because the consistency of two STELLARIS 5 units maximizes efficiency for the user group in training, operating, and scheduling time on the instruments. In the Cellular Imaging Core, the requested STELLARIS 5 will be fully supported financially by CHLA and managed by an experienced PhD-level microscopist. This instrument will greatly benefit the entire confocal user base at CHLA through the ability to generate more imaging data than either of the two old systems, allowing us to meet the long-term objective of illuminating how organs develop and how aberrant structures lead to pediatric disease.

项目摘要 洛杉矶儿童医院(CHLA)申请资金购买徕卡Stellaris 5 Conocus 显微镜将安装在萨班研究所已建立的细胞成像核心设施中 (TSRI)，美国为数不多的几个独立的研究中心之一，科学研究与 临床护理，专为儿童而设。我们的调查人员依靠共聚焦显微镜来探测 发育和疾病的细胞和分子机制，跨越器官系统，在不同的 儿科人口。这一要求有很强的理由。首先，有两个蔡司7系列共焦 细胞成像核心中的显微镜(LSM 710和700)，已有10年以上的历史，技术上过时， 并在其使用寿命即将结束时；蔡司将在2年内停止为其提供服务合同。 其次，LSM 700只有2个通道，使用过时的检测技术，机械上非常 慢的。第三，LSM 710变得不可靠，不起作用的时间大大增加。在.之后 LSM 710的一系列全面故障威胁到了正在进行的研究项目，我们能够 获得机构支持，以获得徕卡Stellaris 5共焦；于2020年12月安装。新的 仪器迎来了下一代技术的细胞成像核心，导致了高需求 以及我们多样化的用户群的高满意度。单靠星座本身是不可能的 支持我们的用户每年3000多个小时的共焦成像，以推动他们的NIH资助 项目，也无法支持更多正在招聘到中情局并将需要这样做的调查人员 技术来建立他们的独立研究计划。因此，该机构为 最初的Stellaris，但在此之前非常需要支持来购买第二个Stellaris 5 Conocus 蔡司的设备变得无法使用和过时。在详细测试了4种共焦模型(徕卡、尼康、 奥林巴斯和蔡司)，我们确定Stellaris 5是CHLA调查人员的最佳选择，具有固定的- 样品应用，因为它的4个灵敏的混合光探测器(HYD S)，每个都可以完全与 1 nm的精度在400-850 nm的范围内。它也是最适合的，因为两个Stellaris 5的一致性 Units可最大限度地提高用户组在培训、操作和安排仪器时间方面的效率。在……里面 蜂窝成像核心，请求的Stellaris 5将完全由CHLA和 由经验丰富的博士级显微镜专家管理。这台仪器将使整个共焦系统受益匪浅。 CHLA的用户群通过生成比两个旧系统中的任何一个都更多的成像数据的能力， 使我们能够达到阐明器官如何发育和结构如何异常的长期目标 会导致儿科疾病。