Intra-and Inter-Species Communication in Bacteria

描述(申请人提供)：这项研究的长期目标是探索细菌用于物种内和物种间细胞间交流的分子机制。这项建议的目的是鉴定和研究哈维氏弧菌的信号转导途径，该信号转导途径将细胞外群体感应信号分子Al-1和Al-2的检测与靶基因反应联系起来。对这个细胞-细胞通信系统中涉及的基因、蛋白质、蛋白质相互作用、蛋白质修饰和信号分子的定义将导致分子理解信号是如何被检测到的，以及这些信息是如何被整合、处理和传递以控制群落尺度上的行为的。分析哈维氏弧菌的群体感应信号电路对于了解细菌如何感知和反应环境，它们如何对时间上一致的线索做出反应，以及它们如何相互沟通以协调行为应该是有价值的。由于调控过程是复杂的，涉及种内、种间、细胞内和细胞间的信号传递，应该揭示有趣的新机制。

DESCRIPTION (provided by applicant): The long-term goal of this research is to explore the molecular mechanisms that bacteria use for intra-and inter-species cell-cell communication. The objective of this proposal is to identify and study the model Vibrio harveyi signal transduction pathway that links detection of extracellular quorum sensing signal molecules termed Al-I and Al-2 to target gene responses. Definition of the genes, proteins, protein-protein interactions, protein modifications and signaling molecules involved in this cell-cell communication system will lead to a molecular understanding of how signals are detected and how this information is integrated, processed, and transduced to control behavior on a community scale. Analyzing the V. harveyi quorum sensing signaling circuit should be valuable for understanding how bacteria perceive and react to their environment, how they respond to temporally coincident cues, and how they communicate with one another to coordinate behavior. Since the regulatory process is complex and involves both intra-species, inter-species, intra-cellular and inter-cellular signal transmission, interesting new mechanisms should be revealed