Intra-and Inter-Species Communication in Bacteria

描述（由申请人提供）：本研究的长期目标是探索细菌用于物种内和物种间细胞-细胞通信的分子机制。本研究的目的是鉴定和研究哈维弧菌信号转导通路模型，该信号转导通路将细胞外群体感应信号分子Al-I和Al-2的检测与靶基因反应联系起来。定义细胞-细胞通信系统中涉及的基因、蛋白质、蛋白质-蛋白质相互作用、蛋白质修饰和信号分子将导致对信号如何被检测以及这些信息如何被整合、处理和转导以控制社区规模行为的分子理解。分析V. harveyi群体感应信号电路对于理解细菌如何感知和对环境做出反应，它们如何对暂时一致的线索做出反应，以及它们如何相互沟通以协调行为是有价值的。由于调控过程复杂，涉及种内、种间、细胞内和细胞间的信号传递，因此应该揭示有趣的新机制

DESCRIPTION (provided by applicant): The long-term goal of this research is to explore the molecular mechanisms that bacteria use for intra-and inter-species cell-cell communication. The objective of this proposal is to identify and study the model Vibrio harveyi signal transduction pathway that links detection of extracellular quorum sensing signal molecules termed Al-I and Al-2 to target gene responses. Definition of the genes, proteins, protein-protein interactions, protein modifications and signaling molecules involved in this cell-cell communication system will lead to a molecular understanding of how signals are detected and how this information is integrated, processed, and transduced to control behavior on a community scale. Analyzing the V. harveyi quorum sensing signaling circuit should be valuable for understanding how bacteria perceive and react to their environment, how they respond to temporally coincident cues, and how they communicate with one another to coordinate behavior. Since the regulatory process is complex and involves both intra-species, inter-species, intra-cellular and inter-cellular signal transmission, interesting new mechanisms should be revealed