Intra-and Inter-Species Communication in Bacteria

细菌的种内和种间通讯

• 批准号: 6931936
• 负责人: BONNIE L BASSLER
• 金额: $ 39.35万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6931936
• 关键词: Vibrio; bacterial genetics; bacterial proteins; biological signal transduction; cell cell interaction; functional /structural genomics; fusion gene; inhibitor /antagonist; microorganism interaction; molecular cloning; protein protein interaction; reporter genes; stimulant /agonist

DESCRIPTION (provided by applicant): The long-term goal of this research is to explore the molecular mechanisms that bacteria use for intra-and inter-species cell-cell communication. The objective of this proposal is to identify and study the model Vibrio harveyi signal transduction pathway that links detection of extracellular quorum sensing signal molecules termed Al-I and Al-2 to target gene responses. Definition of the genes, proteins, protein-protein interactions, protein modifications and signaling molecules involved in this cell-cell communication system will lead to a molecular understanding of how signals are detected and how this information is integrated, processed, and transduced to control behavior on a community scale. Analyzing the V. harveyi quorum sensing signaling circuit should be valuable for understanding how bacteria perceive and react to their environment, how they respond to temporally coincident cues, and how they communicate with one another to coordinate behavior. Since the regulatory process is complex and involves both intra-species, inter-species, intra-cellular and inter-cellular signal transmission, interesting new mechanisms should be revealed

描述（由申请人提供）：这项研究的长期目标是探索细菌用于内部和种间细胞间细胞间通信的分子机制。该提案的目的是识别和研究模型Harveyi信号转导途径，该途径将所谓的Al-I和Al-2的细胞外群体传感信号分子与靶基因响应联系起来。该细胞 - 细胞通信系统中涉及的基因，蛋白质，蛋白质蛋白质相互作用，蛋白质修饰和信号分子的定义将导致分子对检测到信号的检测方式以及该信息的整合，处理和转移到社区规模上的行为。分析V. harveyi Quorum感应信号传导电路对于了解细菌如何感知和对环境的反应，它们如何应对暂时性的提示以及它们如何相互沟通以协调行为应该是有价值的。由于调节过程是复杂的，并且涉及种类内，种间，细胞内和细胞间信号传递，因此应揭示有趣的新机制