Drosophila melanogaster p53 during gonadogenesis

描述（由申请人提供）：生殖系发育是一个高度进化保守的过程，最终产生精子或卵子。这个过程的一个潜在调节因子是肿瘤抑制基因p53。在配子体发生过程中，已经提出p53通过介导细胞凋亡和细胞周期阻滞来监视生殖细胞。此外，最近有报道称，p53的果蝇同源基因（Dmp53）在配子体发生前在生殖细胞中表达。这种表达模式与从蛤蜊到人类等生物体中所见的表达模式一致。因此，我们提出p53也可能在生殖细胞发育的早期阶段发挥生殖细胞监测的作用，为了验证这一假设，我们打算以果蝇（Drosophila Melanogaster）作为模型系统来研究p53在早期生殖细胞发育中的作用。具体来说，我们将进行(1)全面表征Dmp53在种系发育过程中的表达，包括分析调节其表达的体细胞线索，(2)分析Dmp53在种系发育过程中对细胞凋亡、细胞周期进程和生殖细胞成熟的影响，以及(3)检查Dmp53直接介导种系监测的能力。总之，这些研究将深入了解Dmp53在生殖细胞发育和种系选择中的作用。

DESCRIPTION (provided by applicant): Germline development is a highly evolutionarily conserved process, which, ultimately, gives rise to either sperm or eggs. One potential regulator of this process is the tumor suppressor gene p53. During gametogenesis, it has been proposed that p53 functions in surveillance of germ cells by mediating apoptosis and cell cycle arrest. In addition, it has been recently reported that the Drosophila homolog of p53 (Dmp53) is expressed in germ cells prior to the onset of gametogenesis. This expression pattern is consistent with that seen in organisms ranging from clams to humans. Therefore, we propose that p53 may also perform a role in germline surveillance during earlier stages of development to test this hypothesis, we intend to use Drosophila Melanogaster, as a model system to study the role of p53 during early germ cell development. Specifically, we will undertake (1) a thorough characterization of Dmp53 expression during germline development, including an analysis of somatic cues regulating its expression, (2) an analysis of Dmp53's impact on apoptosis, cell cycle progression and germ cell maturation during germline development, and (3) an examination of Dmp53's ability to directly mediate germline surveillance. Together, these studies will provide insight into the role of Dmp53 during germ cell development and germline selection.