Drosophila melanogaster p53 during gonadogenesis

性腺发生过程中的果蝇 p53

基本信息

批准号：
6767808
负责人：
MATTHEW J WAWERSIK
金额：
$ 4.89万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-07-01 至 2005-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6767808
关键词：
DNA damage Drosophilidae X ray apoptosis cell cycle cell proliferation confocal scanning microscopy embryogenesis fluorescent in situ hybridization gametogenesis gonads green fluorescent proteins p53 gene /protein postdoctoral investigator protein structure function reproductive development terminal nick end labeling

项目摘要

DESCRIPTION (provided by applicant): Germline development is a highly evolutionarily conserved process, which, ultimately, gives rise to either sperm or eggs. One potential regulator of this process is the tumor suppressor gene p53. During gametogenesis, it has been proposed that p53 functions in surveillance of germ cells by mediating apoptosis and cell cycle arrest. In addition, it has been recently reported that the Drosophila homolog of p53 (Dmp53) is expressed in germ cells prior to the onset of gametogenesis. This expression pattern is consistent with that seen in organisms ranging from clams to humans. Therefore, we propose that p53 may also perform a role in germline surveillance during earlier stages of development to test this hypothesis, we intend to use Drosophila Melanogaster, as a model system to study the role of p53 during early germ cell development. Specifically, we will undertake (1) a thorough characterization of Dmp53 expression during germline development, including an analysis of somatic cues regulating its expression, (2) an analysis of Dmp53's impact on apoptosis, cell cycle progression and germ cell maturation during germline development, and (3) an examination of Dmp53's ability to directly mediate germline surveillance. Together, these studies will provide insight into the role of Dmp53 during germ cell development and germline selection.

描述（由申请人提供）：种系发育是一个高度进化的过程，最终会产生精子或卵。该过程的一个潜在调节剂是肿瘤抑制基因p53。在配子发生过程中，已经提出p53通过介导凋亡和细胞周期停滞来监测生殖细胞。此外，最近有报道说，在配子发生开始之前，在生殖细胞中表达了p53的果蝇同源物（DMP53）。这种表达模式与从蛤到人类到人类的生物体中所见。因此，我们建议p53在早期发育阶段还可以在生殖线监测中发挥作用，以检验该假设，我们打算使用果蝇Melanogaster作为模型系统，以研究p53在早期生殖细胞发育中的作用。具体而言，我们将进行（1）在生殖线发育过程中对DMP53表达的透彻表征，包括对调节其表达的体细胞提示的分析，（2）分析DMP53对DMP53在生殖线发育过程中的凋亡，细胞周期进展和生殖细胞成熟的影响的分析，以及（3）对DMP53直接介导的培养基的能力。总之，这些研究将洞悉DMP53在生殖细胞发育和种系选择中的作用。