Drosophila melanogaster p53 during gonadogenesis

描述(申请人提供)：生殖系发育是一个在进化上高度保守的过程，最终产生精子或卵子。这一过程的一个潜在调节因素是肿瘤抑制基因p53。在配子发生过程中，人们认为P53通过介导细胞凋亡和细胞周期停滞而在生殖细胞监测中发挥作用。此外，最近有报道称，果蝇的P53同源物(DmP53)在配子发生开始之前就在生殖细胞中表达。这种表达模式与从文蛤到人类的各种生物体中的表达模式一致。因此，我们认为P53也可能在早期发育阶段的生殖系监测中发挥作用。为了验证这一假说，我们打算以黑腹果蝇为模型系统，研究P53在早期生殖细胞发育中的作用。具体地说，我们将进行(1)全面表征Dmp53在生殖系发育过程中的表达，包括分析调控其表达的体细胞线索，(2)分析Dmp53在生殖系发育过程中对细胞凋亡、细胞周期进展和生殖细胞成熟的影响，以及(3)检测Dmp53的S直接调解生殖系监测的能力。总之，这些研究将深入了解Dmp53在生殖细胞发育和生殖系选择中的作用。

DESCRIPTION (provided by applicant): Germline development is a highly evolutionarily conserved process, which, ultimately, gives rise to either sperm or eggs. One potential regulator of this process is the tumor suppressor gene p53. During gametogenesis, it has been proposed that p53 functions in surveillance of germ cells by mediating apoptosis and cell cycle arrest. In addition, it has been recently reported that the Drosophila homolog of p53 (Dmp53) is expressed in germ cells prior to the onset of gametogenesis. This expression pattern is consistent with that seen in organisms ranging from clams to humans. Therefore, we propose that p53 may also perform a role in germline surveillance during earlier stages of development to test this hypothesis, we intend to use Drosophila Melanogaster, as a model system to study the role of p53 during early germ cell development. Specifically, we will undertake (1) a thorough characterization of Dmp53 expression during germline development, including an analysis of somatic cues regulating its expression, (2) an analysis of Dmp53's impact on apoptosis, cell cycle progression and germ cell maturation during germline development, and (3) an examination of Dmp53's ability to directly mediate germline surveillance. Together, these studies will provide insight into the role of Dmp53 during germ cell development and germline selection.