Axonal spiking patterns during high-frequency firing

描述（由申请人提供）：浦肯野细胞作为小脑皮层的唯一输出，负责处理来自大脑不同区域的信息并将这些信息传递给小脑核的细胞。虽然已知信号处理发生在树突和体细胞中，但对浦肯野细胞轴突中可能发生的处理知之甚少。具体来说，我们对浦肯野细胞体细胞和树突在刺激攀爬纤维（即复杂的spike）过程中观察到的大量去极化所导致的轴突动作电位模式感兴趣。此外，高频放电和广泛分支可能降低轴突传递的可靠性。利用电生理和成像技术，我们提出确定浦肯野细胞轴突在高频和突发放电期间的传递函数。我们还建议测试动作电位在高度分叉的侧支轴突和末端投射到小脑深部核的传播的可靠性。

DESCRIPTION (provided by applicant): As the sole output from the cerebellar cortex, Purkinje cells are responsible for processing information from diverse brain regions and delivering this information to the cells of the cerebellar nucleus. Although signal processing is known to occur in the dendrites and the soma, little is known about the processing that may also occur in the axons of Purkinje cells. Specifically, we are interested in the pattern of axonal action potentials that result from the massive depolarization observed in Purkinje cell somata and dendrites during stimulation of climbing fibers (i.e. complex spike). Moreover, high-frequency firing and extensive branching may reduce the reliability of transmission in axons. Using electrophysiological and imaging techniques, we propose to determine the transfer function of Purkinje cell axons in periods of high-frequency and burst firing. We also propose to test the reliability of action potential propagation throughout the highly ramifying collateral axons and terminal projections to the deep cerebellar nucleus.